Clonal expansion within clonal complex 2 and spread of vancomycin-resistant plasmids among different genetic lineages of Enterococcus faecalis from Portugal

Journal of Antimicrobial Chemotherapy

Volumn 63, Issue 6, 2009, Pages 1104-1111

  Freitas, Ana R ^a,b   Novais, Carla ^a,c   Ruiz Garbajosa, Patricia ^b,d,e   Coque, Teresa M ^b,d,e   Peixe, Luísa ^a  

^a UNIVERSITY OF PORTO   (Portugal)

^b HOSPITAL RAMÓN Y CAJAL   (Spain)

^c UNIVERSIDADE FERNANDO PESSOA   (Portugal)

^d CIBER EPIDEMIOLOGÍA Y SALUD PÚBLICA CIBERESP   (Spain)

^e CSIC   (Spain)

Author keywords

CC2; CC21; Enterococci; MLST; Mobile elements; ST16; VRE

Indexed keywords

CHLORAMPHENICOL; CIPROFLOXACIN; ERYTHROMYCIN; GENTAMICIN; KANAMYCIN; STREPTOMYCIN; TEICOPLANIN; TETRACYCLINE; VANCOMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CLUSTER ANALYSIS; ENTEROCOCCUS FAECALIS; EPIDEMIC; MICROBIAL DIVERSITY; MULTILOCUS SEQUENCE TYPING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PORTUGAL; PULSED FIELD GEL ELECTROPHORESIS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEQUENCE ANALYSIS;

ANIMALS; BACTERIAL TYPING TECHNIQUES; CLUSTER ANALYSIS; DNA FINGERPRINTING; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; ELECTROPHORESIS, GEL, PULSED-FIELD; ENTEROCOCCUS FAECALIS; GENE TRANSFER, HORIZONTAL; GENOTYPE; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; PLASMIDS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; PORTUGAL; VANCOMYCIN RESISTANCE; VIRULENCE FACTORS;

EID: 65749093461     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkp103     Document Type: Article

References (30)

1. Murray BE. Vancomycin resistant enterococcal infections. N Engl J Med 2000; 342: 710-21.
2. Top J, Willems R, Blok H et al. Ecological replacement of Enterococcus faecalis by multiresistant clonal complex 17 Enterococcus faecium. Clin Microbiol Infect 2007; 13: 316-9.
3. Flannagan SE, Chow JW, Donabedian SM et al. Plasmid content of a vancomycin-resistant Enterococcus faecalis isolate from a patient also colonized by Staphylococcus aureus with a VanA phenotype. Antimicrob Agents Chemother 2003; 47: 3954-9.
4. Ruiz-Garbajosa P, Bonten MJ, Robinson DA et al. Multilocus sequence typing scheme for Enterococcus faecalis reveals hospital-adapted genetic complexes in a background of high rates of recombination. J Clin Microbiol 2006; 44: 2220-8.
5. Nallapareddy SR, Wenxiang H, Weinstock GM et al. Molecular characterization of a widespread, pathogenic, and antibiotic resistance-receptive Enterococcus faecalis lineage and dissemination of its putative pathogenicity island. J Bacteriol 2005; 187: 5709-18.
6. Leavis HL, Bonten MJ, Willems RJ. Identification of high-risk enterococcal clonal complexes: Global dispersion and antibiotic resistance. Curr Opin Microbiol 2006; 9: 454-60.
7. McBride SM, Fischetti VA, Leblanc DJ et al. Genetic diversity among Enterococcus faecalis. PLoS ONE 2007; 2: E582.
8. Novais C, Coque TM, Sousa JC et al. Local genetic patterns within a vancomycin-resistant Enterococcus faecalis clone isolated in three hospitals in Portugal. Antimicrob Agents Chemother 2004; 48: 3613-7.
9. Novais C, Coque TM, Ferreira H et al. Environmental contamination with vancomycin-resistant enterococci from hospital sewage in Portugal. Appl Environ Microbiol 2005; 71: 3364-8.
10. Novais C, Coque TM, Costa MJ et al. High occurrence and persistence of antibiotic-resistant enterococci in poultry food samples in Portugal. J Antimicrob Chemother 2005; 56: 1139-43.
11. Novais C, Coque TM, Sousa JC et al. Antimicrobial resistance among faecal enterococci from healthy individuals in Portugal. Clin Microbiol Infect 2006; 12: 1131-4.
12. Dukta-Malen S, Evers S, Courvalin P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 1995; 33: 24-7.
13. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Sixteenth Informational Supplement M100-S16. CLSI, Wayne, PA, USA, 2006.
14. Vankerckhoven V, van Autgaerden T, Vael C et al. Development of a multiplex PCR for the detection of asa1, gelE, cylA, esp and hyl genes in enterococci and survey for virulence determinants among European hospital isolates of Enterococcus faecium. J Clin Microbiol 2004; 42: 4473-9.
15. Tenover FC, Arbeit RD, Goering RV et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33 2233-9.
16. Feil EJ, Li BC, Aanensen DM et al. eBURST: Inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 2004; 186: 1518-30.
17. Woodford N, Adebiyi AA, Palepou MI et al. Diversity of VanA glycopeptide resistance elements in enterococci from humans and nonhuman sources. Antimicrob Agents Chemother 1998; 42: 502-8.
18. Novais C, Freitas AR, Sousa JC et al. Diversity of Tn1546 and its role in the dissemination of vancomycin-resistant enterococci in Portugal. Antimicrob Agents Chemother 2008; 52: 1001-8.
19. Barton BM, Harding GP, Zuccarelli AJ. A general method for detecting and sizing large plasmids. Anal Biochem 1995; 226: 235-40.
20. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989.
21. Handwerger S, Skoble J, Discotto LF et al. Heterogeneity of the vanA gene cluster in clinical isolates of enterococci from the northeastern United States. Antimicrob Agents Chemother 1995; 39: 362-8.
22. Kawalec M, Pietras Z, Danilowicz E et al. Clonal structure of Enterococcus faecalis isolated from Polish hospitals: The characterization of epidemic clones. J Clin Microbiol 2007; 45 147-53.
23. Mato R, Almeida F, Pires R et al. Assessment of high-level gentamicin and glycopeptide-resistant Enterococcus faecalis and E. faecium clonal structure in a Portuguese hospital over a 3-year period. Eur J Clin Microbiol Infect Dis 2009; doi:10.1007/ s10096-009-0704-x.
24. Agersø Y, Lester CH, Porsbo LJ et al. Vancomycin-resistant Enterococcus faecalis isolates from a Danish patient and two healthy human volunteers are possibly related to isolates from imported turkey meat. J Antimicrob Chemother 2008; 62: 844-5.
25. Donabedian SM, Thal LA, Hershberger E et al. Molecular characterization of gentamicin-resistant enterococci in the United States: Evidence of spread from animals to humans through food. J Clin Microbiol 2003; 41: 1109-13.
26. Manson JM, Keis S, Smith JM et al. A clonal lineage of VanA-type Enterococcus faecalis predominates in vancomycinresistant enterococci isolated in New Zealand. Antimicrob Agents Chemother 2003; 47 204-10.
27. Manson JM, Keis S, Smith JM et al. Characterization of a vancomycin-resistant Enterococcus faecalis (VREF) isolate from a dog with mastitis: Further evidence of a clonal lineage of VREF in New Zealand. J Clin Microbiol 2003; 41: 3331-3.
28. Ozawa Y, Tanimoto K, Nomura T et al. Vancomycin-resistant enterococci in humans and imported chickens in Japan. Appl Environ Microbiol 2002; 68: 6457-61.
29. Shankar N, Baghdayan AS, Willems R et al. Presence of pathogenicity island genes in Enterococcus faecalis isolates from pigs in Denmark. J Clin Microbiol 2006; 44: 4200-3.
30. Petersen A, Christensen H, Philipp HC et al. Clonality of Enterococcus faecalis associated with amyloid arthropathy in chickens evaluated by multilocus sequence typing (MLST). Vet Microbiol 2009; 134: 392-5.