Whole-mount in situ hybridization of short-tailed fruit bat (Carollia perspicillata) embryos with RNA probes

Cold Spring Harbor Protocols

Volumn 4, Issue 3, 2009, Pages

  Rasweiler IV, John J ^a   Cretekos, Chris J ^b   Behringer, Richard R ^c  

^a SUNY Downstate Medical Center   (United States)

^b Nucl Eng Idaho State University   (United States)

^c UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL EMBRYO; ARTICLE; BAT; EMBRYO DEVELOPMENT; GENE EXPRESSION; IN SITU HYBRIDIZATION; NONHUMAN; RNA PROBE;

CAROLLIA PERSPICILLATA;

EID: 65749087434     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5164     Document Type: Article

References (7)

1. Braissant, O. and Wahli, W. 1998. A simplified in situ protocol using non-radioactive probes to detect abundant and rare mRNAs. Biochemica 1: 10-16.
2. Cretekos, C.J., Weatherbee, S.D., Chen, C.-H., Badwaik, N.K., Niswander, L., Behringer, R.R., and Rasweiler IV, J.J. 2005. Embryonic staging system for the short-tailed fruit bat, Carollia perspicillata, a model organism for the mammalian order Chiroptera, based upon timed pregnancies in captive-bred animals. Dev. Dyn. 233: 721-738.
3. Harland, R.M. 1991. In situ hybridization: An improved whole-mount method for Xenopus embryos. Methods Cell Biol. 36: 685-695.
4. Rasweiler IV, J.J., Cretekos, C.J., and Behringer, R.R. 2009a. Fixation and storage of embryos from short-tailed fruit bat (Carollia perspicillata). Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5163.
5. Rasweiler IV, J.J., Cretekos, C.J., and Behringer, R.R. 2009b. Whole-mount immunohistochemistry of short-tailed fruit bat (Carollia perspicillata). Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5167.
6. Rasweiler IV, J.J., Cretekos, C.J., and Behringer, R.R. 2009c. The short-tailed fruit bat Carollia perspicillata: A model for studies in reproduction and development. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.emo118.
7. Wilkinson, D.G. 1992. In situ hybridization: A practical approach. Oxford University Press, New York.