Regulating protein stability in mammalian cells using small molecules

Cold Spring Harbor Protocols

Volumn 4, Issue 3, 2009, Pages

  Hagan, Emily L ^a   Banaszynski, Laura A ^a   Chen, Ling Chun ^a   Maynard Smith, Lystranne A ^a   Wandless, Thomas J ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYBRID PROTEIN;

ANIMAL CELL; ARTICLE; BIOLOGICAL ACTIVITY; CELL LINE; CONTROLLED STUDY; HUMAN; HUMAN CELL; MAMMAL CELL; MOLECULAR CLONING; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN STABILITY; REGULATORY MECHANISM;

MAMMALIA;

EID: 65749086059     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5172     Document Type: Article

References (5)

1. Banaszynski, L.A., Chen, L.C., Maynard-Smith, L.A., Ooi, A.G.L., and Wandless, T.J. 2006. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell 126: 995-1004.
2. Banaszynski, L.A., Sellmyer, M.A., Contag, C.H., Wandless, T.J., and Thorne, S.H. 2008. Chemical control of protein stability and function in living animals. Nat. Med. 14: 1123-1127.
3. Chu, B.W., Banaszynski, L.A., Chen, L.C., and Wandless, T.J. 2008. Recent progress with FKBP-derived destabilizing domains. Bioorg. Med. Chem. Lett. 18: 5941-5944.
4. Maynard-Smith, L.A., Chen, L.C., Banaszynski, L.A., Ooi, A.G.L., and Wandless, T.J. 2007. A directed approach for engineering conditional protein stability using biologically silent small molecules. J. Biol. Chem. 282: 24866-24872.
5. Sellmyer, M.A., Thorne, S.H., Banaszynski, L.A., Contag, C.H., and Wandless, T.J. 2009. A general method for conditional regulation of protein stability in living animals. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5173.