Formaldehyde fixation of cells does not greatly reduce the ability to amplify cellular DNA

Analytical Biochemistry

Volumn 390, Issue 1, 2009, Pages 94-96

  Gavrilov, Alexey ^a   Razin, Sergey V ^a  

^a INSTITUTE OF GENE BIOLOGY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

FORMALDEHYDE; POLYMERASE CHAIN REACTION;

DNA AMPLIFICATION; DNA-PROTEIN INTERACTION; IN-VIVO; POLYMERASE CHAIN REACTION TECHNIQUES;

DNA;

CELL DNA; FORMALDEHYDE;

ARTICLE; CHROMATIN; DNA DAMAGE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN INTERACTION; REDUCTION;

EID: 65649145185     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.04.018     Document Type: Article

References (9)

1. Orlando V., Strutt H., and Paro R. Analysis of chromatin structure by in vivo formaldehyde cross-linking. Methods 11 (1997) 205-214
2. Solomon M.J., and Varshavsky A. Formaldehyde-mediated DNA-protein crosslinking: a probe for in vivo chromatin structures. Proc. Natl. Acad. Sci. USA 82 (1985) 6470-6474
3. Dekker J., Rippe K., Dekker M., and Kleckner N. Capturing chromosome conformation. Science 295 (2002) 1306-1311
4. Srinivasan M., Sedmak D., and Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am. J. Pathol. 161 (2002) 1961-1971
5. Douglas M.P., and Rogers S.O. DNA damage caused by common cytological fixatives. Mutat. Res. 401 (1998) 77-88
6. Beug H., Doederlein G., Freudenstein C., and Graf T. Erythroblast cell lines transformed by a temperature-sensitive mutant of avian erythroblastosis virus: a model system to study erythroid differentiation in vitro. J. Cell. Physiol. Suppl. 1 (1982) 195-207
7. Gavrilov A.A., and Razin S.V. Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs. Nucleic Acids Res. 36 (2008) 4629-4640
8. Prioleau M.N., Nony P., Simpson M., and Felsenfeld G. An insulator element and condensed chromatin region separate the chicken β-globin locus from an independently regulated erythroid-specific folate receptor gene. EMBO J. 18 (1999) 4035-4048
9. Flint J., Tufarelli C., Peden J., Clark K., Daniels R.J., Hardison R., Miller W., Philipsen S., Tan-Un K.C., McMorrow T., Frampton J., Alter B.P., Frischauf A.M., and Higgs D.R. Comparative genome analysis delimits a chromosomal domain and identifies key regulatory elements in the α-globin cluster. Hum. Mol. Genet. 10 (2001) 371-382