A method for the identification of proteins secreted by lactic acid bacteria grown in complex media

FEMS Microbiology Letters

Volumn 295, Issue 2, 2009, Pages 226-229

  Sánchez, Borja ^a   Chaignepain, Sthéphane ^a   Schmitter, Jean Marie ^a   Urdaci, María C ^a  

^a UNIVERSITÉ DE BORDEAUX   (France)

Author keywords

Lactic acid bacteria; Protein precipitation; Secreted proteins

Indexed keywords

LACTIC ACID; PEPTIDE DERIVATIVE; PROBIOTIC AGENT; TRICHLOROACETIC ACID;

ACID PRECIPITATION; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN SECRETION; TANDEM MASS SPECTROMETRY;

EID: 65649125256     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2009.01599.x     Document Type: Article

References (18)

1. Buck BL, Altermann E, Svingerud T Klaenhammer TR (2005) Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM. Appl Environ Microb 71 : 8344 8351. (Pubitemid 41803962)
2. Gardy JL, Laird MR, Chen F, Rey S, Walsh CJ, Ester M Brinkman FS (2005) PSORTb v.2.0: expanded prediction of bacterial protein subcellular localization and insights gained from comparative proteome analysis. Bioinformatics 21 : 617 623. (Pubitemid 40424787)
3. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 : 680 685.
4. Lee SK, Kim YB Ji GE (1997) Note: purification of amylase secreted from Bifidobacterium adolescentis. J Appl Microbiol 83 : 267 272. (Pubitemid 27396319)
5. Peant B LaPointe G (2004) Identification and characterization of a conserved nuclease secreted by strains of the Lactobacillus casei group. J Appl Microbiol 96 : 367 374. (Pubitemid 38223562)
6. Rodrigues LR, Teixeira JA Oliveira R (2006) Low-cost fermentative medium for biosurfactant production by probiotic bacteria. Biochem Eng J 32 : 135 142.
7. Sánchez B, Champomier-Verges MC, Anglade P, Baraige F, de los Reyes-Gavilan CG, Margolles A Zagorec M (2008) A preliminary analysis of Bifidobacterium longum exported proteins by two-dimensional electrophoresis. J Mol Microb Biotech 14 : 74 79. (Pubitemid 350022335)
8. Siezen R, Boekhorst J, Muscariello L, Molenaar D, Renckens B Kleerebezem M (2006) Lactobacillus plantarum gene clusters encoding putative cell-surface protein complexes for carbohydrate utilization are conserved in specific gram-positive bacteria. BMC Genomics 7 : 126. (Pubitemid 44202742)
9. Todorov SD Dicks LM (2004) Influence of growth conditions on the production of a bacteriocin by Lactococcus lactis subsp. lactis ST34BR, a strain isolated from barley beer. J Basic Microb 44 : 305 316. (Pubitemid 39246759)
10. Trost M, Wehmhoner D, Karst U, Dieterich G, Wehland J Jansch L (2005) Comparative proteome analysis of secretory proteins from pathogenic and nonpathogenic Listeria species. Proteomics 5 : 1544 1557. (Pubitemid 40593263)
11. Turner MS, Timms P, Hafner LM Giffard PM (1997) Identification and characterization of a basic cell surface-located protein from Lactobacillus fermentum BR11. J Bacteriol 179 : 3310 3316. (Pubitemid 27203008)
12. Turner MS, Hafner LM, Walsh T Giffard PM (2004) Identification and characterization of the novel LysM domain-containing surface protein Sep from Lactobacillus fermentum BR11 and its use as a peptide fusion partner in Lactobacillus and Lactococcus. Appl Environ Microb 70 : 3673 3680. (Pubitemid 38745917)
13. van Asseldonk M, Rutten G, Oteman M, Siezen RJ, de Vos WM Simons G (1990) Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363. Gene 95 : 155 160.
14. van Pijkeren JP, Canchaya C, Ryan KA et al 2006) Comparative and functional analysis of sortase-dependent proteins in the predicted secretome of Lactobacillus salivarius UCC118. Appl Environ Microb 72 : 4143 4153. (Pubitemid 43894881)
15. van Wely KH, Swaving J, Freudl R Driessen AJ (2001) Translocation of proteins across the cell envelope of Gram-positive bacteria. FEMS Microbiol Rev 25 : 437 454. (Pubitemid 32778468)
16. Weisburg WG, Barns SM, Pelletier DA Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 : 697 703.
17. Xiao ZJ, Liu PH, Qin JY Xu P (2007) Statistical optimization of medium components for enhanced acetoin production from molasses and soybean meal hydrolysate. Appl Microbiol Biot 74 : 61 68. (Pubitemid 46183468)
18. Yan F, Cao H, Cover TL, Whitehead R, Washington MK Polk DB (2007) Soluble proteins produced by probiotic bacteria regulate intestinal epithelial cell survival and growth. Gastroenterol 132 : 562 575. (Pubitemid 46274659)