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Volumn 4, Issue 1, 2009, Pages

In vitro sumoylation of recombinant proteins and subsequent purification for use in enzymatic assays

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; LIGASE; PROTEIN UBC9; RECOMBINANT PROTEIN; SUMO PROTEIN;

EID: 65649116595     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5121     Document Type: Article
Times cited : (6)

References (14)
  • 1
    • 0030728212 scopus 로고    scopus 로고
    • Ubch9 conjugates SUMO but not ubiquitin
    • Desterro, J.M., Thomson, J., and Hay, R.T. 1997. Ubch9 conjugates SUMO but not ubiquitin. FEBS Lett. 417: 297-300.
    • (1997) FEBS Lett , vol.417 , pp. 297-300
    • Desterro, J.M.1    Thomson, J.2    Hay, R.T.3
  • 2
    • 0032135131 scopus 로고    scopus 로고
    • SUMO-1 modification of IκBα inhibits NF-κB activation
    • Desterro, J.M., Rodriguez, M.S., and Hay, R.T. 1998. SUMO-1 modification of IκBα inhibits NF-κB activation. Mol. Cell 2: 233-239.
    • (1998) Mol. Cell , vol.2 , pp. 233-239
    • Desterro, J.M.1    Rodriguez, M.S.2    Hay, R.T.3
  • 3
    • 0033537828 scopus 로고    scopus 로고
    • Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1
    • Desterro, J.M., Rodriguez, M.S., Kemp, G.D., and Hay, R.T. 1999. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem. 274: 10618-10624.
    • (1999) J. Biol. Chem , vol.274 , pp. 10618-10624
    • Desterro, J.M.1    Rodriguez, M.S.2    Kemp, G.D.3    Hay, R.T.4
  • 5
    • 84959181842 scopus 로고    scopus 로고
    • Immunoblotting: Antigen detection using chemiluminescence
    • doi: 10.1101/ pdb.prot4271
    • Harlow, E. and Lane, D. 2006. Immunoblotting: Antigen detection using chemiluminescence. Cold Spring Harb. Protoc. doi: 10.1101/ pdb.prot4271.
    • (2006) Cold Spring Harb. Protoc
    • Harlow, E.1    Lane, D.2
  • 6
    • 0030455748 scopus 로고    scopus 로고
    • A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex
    • Matunis, M.J., Coutavas, E., and Blobel, G. 1996. A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J. Cell Biol. 135: 1457-1470.
    • (1996) J. Cell Biol , vol.135 , pp. 1457-1470
    • Matunis, M.J.1    Coutavas, E.2    Blobel, G.3
  • 7
    • 0037498052 scopus 로고    scopus 로고
    • Conjugation with the ubiquitin-related modifier SUMO-1 regulates the partitioning of PML within the nucleus
    • Müller, S., Matunis, M.J., and Dejean, A. 1998. Conjugation with the ubiquitin-related modifier SUMO-1 regulates the partitioning of PML within the nucleus. EMBO J. 17: 61-70.
    • (1998) EMBO J , vol.17 , pp. 61-70
    • Müller, S.1    Matunis, M.J.2    Dejean, A.3
  • 8
    • 84934437243 scopus 로고    scopus 로고
    • Analysis of sumoylation
    • Pichler, A. 2008. Analysis of sumoylation. Methods Mol. Biol. 446: 131-138.
    • (2008) Methods Mol. Biol , vol.446 , pp. 131-138
    • Pichler, A.1
  • 12
    • 77956276068 scopus 로고    scopus 로고
    • Staining proteins in gels with silver nitrate
    • doi: 10.1101/pdb.prot4727
    • Simpson, R.J. 2007. Staining proteins in gels with silver nitrate. Cold Spring Harb. Protoc. doi: 10.1101/pdb.prot4727.
    • (2007) Cold Spring Harb. Protoc
    • Simpson, R.J.1
  • 14
    • 39449103526 scopus 로고    scopus 로고
    • Sumoylation regulates multiple aspects of mammalian poly(A) polymerase function
    • Vethantham, V., Rao, N., and Manley, J.L. 2008. Sumoylation regulates multiple aspects of mammalian poly(A) polymerase function. Genes & Dev 22: 499-511.
    • (2008) Genes & Dev , vol.22 , pp. 499-511
    • Vethantham, V.1    Rao, N.2    Manley, J.L.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.