Expression analysis of the toll-like receptors in human peripheral blood mononuclear cells

Methods in Molecular Biology

Volumn 517, Issue , 2009, Pages 3-14

  Siednienko, Jakub ^a   Miggin, Sinead M ^a  

^a NATIONAL UNIVERSITY OF IRELAND   (Ireland)

Author keywords

Expression analysis; PBMC; RNA isolation; RT PCR; Toll like receptor

Indexed keywords

BACTERIA (MICROORGANISMS);

COMPLEMENTARY DNA; RNA; TOLL LIKE RECEPTOR;

AGAR GEL ELECTROPHORESIS; ANIMAL; ARTICLE; CELL LINE; CLASSIFICATION; GENE EXPRESSION REGULATION; GENETICS; HUMAN; METABOLISM; MONONUCLEAR CELL; POLYMERASE CHAIN REACTION;

ANIMALS; CELL LINE; DNA, COMPLEMENTARY; ELECTROPHORESIS, AGAR GEL; GENE EXPRESSION REGULATION; HUMANS; LEUKOCYTES, MONONUCLEAR; POLYMERASE CHAIN REACTION; RNA; TOLL-LIKE RECEPTORS;

EID: 65649108426     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-541-1_1     Document Type: Article

References (5)

1. O'Neill, L. A., Fitzgerald, K. A., and Bowie, A. G. (2003) The Toll-IL-1 receptor adaptor family grows to five members. Trends Immunol 24, 286-90.
2. Miggin, S. M. and O'Neill, L. A. (2006) New insights into the regulation of TLR signaling. J Leukoc Biol 80, 220-6. (Pubitemid 44835495)
3. O'Neill, L. A. (2006) How Toll-like receptors signal: what we know and what we don't know. Curr Opin Immunol 18, 3-9. (Pubitemid 43049641)
4. Kurt-Jones, E. A., Sandor, F., Ortiz, Y., Bowen, G. N., Counter, S. L., Wang, T. C., and Finberg, R. W. (2004) Use of murine embryonic fibroblasts to define toll-like receptor activation and specificity. J Endotoxin Res 10, 419-24. (Pubitemid 39618557)
5. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162, 156-9. (Pubitemid 17064313)