Biomolecular characterization and protein sequences of the campanian hadrosaur b. canadensis

Science

Volumn 324, Issue 5927, 2009, Pages 626-631

  Schweitzer, Mary H ^a,b   Zheng, Wenxia ^a   Organ, Chris L ^c   Avci, Recep ^d   Suo, Zhiyong ^d   Freimark, Lisa M ^e   Lebleu, Valerie S ^e,f   Duncan, Michael B ^e,f   Heiden, Matthew G Vander ^g   Neveu, John M ^h   Lane, William S ^h   Cottrell, John S ⁱ   Horner, John R ^j   Cantley, Lewis C ^e,f   Kalluri, Raghu ^e,f   Asara, John M ^e,f  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

^b NORTH CAROLINA MUSEUM OF NATURAL SCIENCES   (United States)

^c HARVARD UNIVERSITY   (United States)

^d MONTANA STATE UNIVERSITY   (United States)

^e BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

^f HARVARD MEDICAL SCHOOL   (United States)

^g DANA FARBER CANCER INSTITUTE   (United States)

^h HARVARD UNIVERSITY   (United States)

ⁱ Matrix Science Ltd   (United Kingdom)

^j Museum of the Rockies   (United States)

more..

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN TYPE 1; OSTEOCALCIN; COLLAGEN; ELASTIN; HEMOGLOBIN; LAMININ; PEPTIDE FRAGMENT; PROTEIN;

CRETACEOUS; DINOSAUR; MICROSTRUCTURE; MOLECULAR ANALYSIS; PHYLOGENETICS; PRESERVATION; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BONE MATRIX; CONTROLLED STUDY; DINOSAUR; FIELD EMISSION SCANNING ELECTRON MICROSCOPY; HADROSAUR; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; ANIMAL; BIRD; CHEMISTRY; CLASSIFICATION; FEMUR; FOSSIL; HISTOLOGY; IMMUNOLOGICAL PROCEDURES; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; OSTEOCYTE; PHYLOGENY; SCANNING ELECTRON MICROSCOPY; SEQUENCE ALIGNMENT; ULTRASTRUCTURE; VASCULARIZATION;

AVES; DINOSAURIA; TYRANNOSAURUS REX;

AMINO ACID SEQUENCE; ANIMALS; BIRDS; BONE DEMINERALIZATION TECHNIQUE; BONE MATRIX; COLLAGEN; DINOSAURS; ELASTIN; FEMUR; FOSSILS; HEMOGLOBINS; IMMUNOLOGIC TECHNIQUES; LAMININ; MASS SPECTROMETRY; MICROSCOPY, ELECTRON, SCANNING; MOLECULAR SEQUENCE DATA; OSTEOCYTES; PEPTIDE FRAGMENTS; PHYLOGENY; PROTEINS; SEQUENCE ALIGNMENT;

EID: 65549134251     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1165069     Document Type: Article

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26. We thank B. Harmon, N. Peterson, and the MOR crew responsible for recovery of MOR 2598; N. Equall and S. Brumfield for microscopy assistance; G. Fisher (Physical Electronics) for PHI nanoTOF assistance; T. Cleland for assistance with gel analyses; X. Yang for help with mass spectrometry sample preparation; S. Stein and Y. Mirokhin for MS Search 2.0 software enhancements; J. Starkey and R. Mecham for antibodies; B. Anderson for Fourier transform infrared spectroscopy access; T. Collier for sample preparation; E. Leithold for access to equipment; J. C. Fountain for support; and the Montana Department of Natural Resources and Conservation for land access. C.L.O. thanks S. V. Edwards for postdoctoral support. We acknowledge funding from NSF [awards EAR 0722702 and EAR 0634136 (J.M.A.) and EAR 0548847 and EAR-0541744 (M.H.S.)]; the David and Lucile Packard Foundation (M.H.S.); United Negro College Fund-Merck Postdoctoral Science Research Fellowship (M.B.D.); the Taplin Funds for Discovery (Harvard Medical School); partial support by NASA-Expermental Project to Simulate Competitive Research under grant NCC5-579 (R.A. and Z.S.); Damon Runyon Cancer Research Foundation (M.G.V.H.); NIH grants DK 55001, DK 62987, AA 13913, DK 61866, and CA 125550; and the Department of Medicine for the Division of Matrix Biology at the Beth Israel Deaconess Medical Center. Correspondence and requests for materials should be addressed to M.H.S. for biochemical and morphological analyses and to J.M.A. for mass spectrometry and molecular phylogenetics analyses.