PCR amplification of a locus with RFLP alleles specific to African honey bees

Biochemical Genetics

Volumn 36, Issue 9-10, 1998, Pages 351-361

  Glenn Hall, H ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

African honey bees; Polymerase chain reaction; Restriction fragment length polymorphism

Indexed keywords

ARTICLE; GENE LOCUS; HONEYBEE; MIGRATION; MOLECULAR GENETICS; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ALLELES; ANIMALS; BEES; DNA PRIMERS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RESTRICTION MAPPING;

APINAE; APIS MELLIFERA; APOIDEA; INSECTA;

EID: 6544274707     PISSN: 00062928     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018797429804     Document Type: Article

References (11)

1. Hall, H. G. (1986). DNA differences found between Africanized and European honeybees. Proc. Natl. Acad. Sci. USA 83:4874.
2. Hall, H. G. (1990). Parental analysis of introgressive hybridization between African and European honeybees using nuclear DNA RFLPs. Genetics 125:611.
3. Hall, H. G. (1991). Genetic characterization of honey bees through DNA analysis. In Spivak, M., Breed, M., and Fletcher, D. J. C. (eds.), The "African" Honey Bee, Westview Press, Boulder, CO, pp. 45-73.
4. Hall, H. G. (1992a). Processes of New World African honeybee spread revealed by DNA studies. Fla. Entomol. 75:51.
5. Hall, H. G. (1992b). Further characterization of nuclear DNA RFLP markers that distinguish African and European honeybees. Arch. Insect Biochem. Physiol. 19:163.
6. Hall, H. G. (1995). Restriction fragment length polymorphism (RFLP) analyses: Distinguishing African and European honeybees. In Clapp, J. P. (ed.), Molecular Methods for Species Identification, Methods in Molecular Biology, Vol. 50, Humana Press, pp. 333-366.
7. Hall, H. G., and McMichael, M. A. (1992). European honey bee (Apis mellifera L.) (Hymenoptera: Apidae) colonies at high elevations in Costa Rica tested for African DNA markers. Bee Sci. 2:25.
8. McMichael, M., and Hall, H. G. (1996). DNA RFLPs at a highly polymorphic locus distinguish European and African subspecies of the honey bee, Apis mellifera L. and suggest geographical origins of New World honey bees. Mol. Ecol. 5:403.
9. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., and Horn, G. T. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487.
10. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
11. Schaffer, H. E., and Sederoff, R. R. (1981). Improved estimation of DNA fragment lengths from agarose gels. Anal. Biochem. 115:113.