Alpha-Sarcin catalytic activity is not required for cytotoxicity

BMC Biochemistry

Volumn 10, Issue 1, 2009, Pages

  Alford, Spencer C ^a   Pearson, Joel D ^b   Carette, Amanda ^a   Ingham, Robert J ^b   Howard, Perry L ^a  

^a UNIVERSITY OF VICTORIA   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA SARCIN; BIOLOGICAL MARKER; MUTANT PROTEIN; RICIN; STRESS ACTIVATED PROTEIN KINASE; ALPHA-SARCIN; FUNGAL PROTEIN; PROTEIN SYNTHESIS INHIBITOR; RECOMBINANT PROTEIN; RIBONUCLEASE;

ANIMAL CELL; ARTICLE; CATALYSIS; CELL DEATH; CELL NUCLEUS; CYTOPLASM; CYTOTOXICITY; CYTOTOXICITY TEST; FEMALE; HUMAN; HUMAN CELL; MUTATION; NONHUMAN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; PROTEIN SYNTHESIS INHIBITION; PROTEIN VARIANT; RIBOSOME; WILD TYPE; AMINO ACID SUBSTITUTION; ASPERGILLUS; CELL LINE; DRUG EFFECT; GENETIC TRANSFECTION; HELA CELL; METABOLISM;

ASPERGILLUS GIGANTEUS;

AMINO ACID SUBSTITUTION; ASPERGILLUS; CELL DEATH; CELL LINE; ENDORIBONUCLEASES; FUNGAL PROTEINS; HELA CELLS; HUMANS; MUTANT PROTEINS; PROTEIN SYNTHESIS INHIBITORS; RECOMBINANT PROTEINS; RICIN; TRANSFECTION;

EID: 65349184525     PISSN: None     EISSN: 14712091     Source Type: Journal    
DOI: 10.1186/1471-2091-10-9     Document Type: Article

References (18)

1. Ribosome inactivating proteins and apoptosis. S Narayanan K Surendranath N Bora A Surolia AA Karande, FEBS Lett 2005 579 1324 1331 10.1016/j.febslet. 2005.01.038 15733836 (Pubitemid 40289175)
2. Study of the interaction between the antitumour protein alpha-sarcin and phospholipid vesicles. M Gasset A Martinez-del-Pozo M Onaderra JG Gavilanes, Biochem J 1989 258 569 575 2706001 (Pubitemid 19075534)
3. Fusion of phospholipid vesicles produced by the anti-tumour protein alpha-sarcin. M Gasset M Onaderra PG Thomas JG Gavilanes, Biochem J 1990 265 815 822 2306215
4. Oligomerization of the cytotoxin alpha-sarcin associated with phospholipid membranes. M Onaderra JM Mancheno J Lacadena lRV De A Martinez-del-Pozo JG Gavilanes, Mol Membr Biol 1998 15 141 144 10.3109/09687689809074525 9859111 (Pubitemid 28542668)
5. Cytotoxic mechanism of the ribotoxin alpha-sarcin. Induction of cell death via apoptosis. N Olmo J Turnay dB Gonzalez dSI Lopez JG Gavilanes MA Lizarbe, Eur J Biochem 2001 268 2113 2123 10.1046/j.1432-1327.2001.02086.x 11277935 (Pubitemid 32861463)
6. Ribotoxic stress response: activation of the stress-activated protein kinase JNK1 by inhibitors of the peptidyl transferase reaction and by sequence-specific RNA damage to the alpha-sarcin/ricin loop in the 28S rRNA. MS Iordanov D Pribnow JL Magun TH Dinh JA Pearson SL Chen BE Magun, Mol Cell Biol 1997 17 3373 3381 9154836 (Pubitemid 27215052)
7. Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin alpha-sarcin. J Lacadena A Martinez-del-Pozo A Martinez-Ruiz JM Perez-Canadillas M Bruix JM Mancheno M Onaderra JG Gavilanes, Proteins 1999 37 3 474 84 10.1002/(SICI)1097- 0134(19991115)37:3<474::AID-PROT14>3.0.CO;2-N 10591106 (Pubitemid 29519736)
8. Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin alpha-sarcin. M Masip J Lacadena JM Mancheno M Onaderra A Martinez-Ruiz A Martinez-del-Pozo JG Gavilanes, Eur J Biochem 2001 268 23 6190 6 10.1046/j.0014-2956.2001.02566.x 11733014 (Pubitemid 33132039)
9. Proteins are cointernalized with virion particles during early infection. MJ Otero L Carrasco, Virology 1987 160 75 80 10.1016/0042-6822(87)90046-8 2820142
10. The ribonuclease activity of the cytotoxin alpha-sarcin. The characteristics of the enzymatic activity of alpha-sarcin with ribosomes and ribonucleic acids as substrates. Y Endo PW Huber IG Wool, J Biol Chem 1983 258 2662 2667 6185500
11. Ribosome inactivation by the toxic lectins abrin and ricin. Kinetics of the enzymic activity of the toxin A-chains. S Olsnes C Fernandez-Puentes L Carrasco D Vazquez, Eur J Biochem 1975 60 281 288 10.1111/j.1432-1033.1975. tb21001.x 1204642
12. Deletion of the NH2-terminal beta-hairpin of the ribotoxin alpha-sarcin produces a nontoxic but active ribonuclease. L Garcia-Ortega M Masip JM Mancheno M Onaderra MA Lizarbe MF Garcia-Mayoral M Bruix A Martinez-del-Pozo JG Gavilanes, J Biol Chem 2002 277 21 18632 9 10.1074/jbc.M200922200 11897788
13. The electrostatic character of the ribosomal surface enables extraordinarily rapid target location by ribotoxins. AV Korennykh JA Piccirilli CC Correll, Nat Struct Mol Biol 2006 13 436 443 16604082 10.1038/nsmb1082 (Pubitemid 43881584)
14. Evidence for the importance of electrostatics in the function of two distinct families of ribosome inactivating toxins. AV Korennykh CC Correll JA Piccirilli, RNA 2007 13 1391 1396 17626843 10.1261/rna.619707 (Pubitemid 47347409)
15. Abrin triggers cell death by inactivating a thiol-specific antioxidant protein. SF Shih YH Wu CH Hung HY Yang JY Lin, J Biol Chem 2001 276 21870 21877 10.1074/jbc.M100571200 11285261 (Pubitemid 37413191)
16. Ribosome depurination is not sufficient for ricin-mediated cell death in Saccharomyces cerevisiae. XP Li M Baricevic H Saidasan NE Tumer, Infect Immun 2007 75 417 428 17101666 10.1128/IAI.01295-06 (Pubitemid 46047918)
17. Molecular dissection of mitogillin reveals that the fungal ribotoxins are a family of natural genetically engineered ribonucleases. R Kao J Davies, J Biol Chem 1999 274 12576 12582 10.1074/jbc.274.18.12576 10212236 (Pubitemid 29215493)
18. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. UK Laemmli, Nature 1970 227 680 685 10.1038/227680a0 5432063