A simple TAE-based method to generate large insert BAC libraries from plant species

Methods in Molecular Biology

Volumn 513, Issue , 2009, Pages 57-80

  Shi, Bu Jun ^a   Perry Gustafson, J ^a   Langridge, Peter ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

BAC library; Barley; Large insert; Phalaris; pIndigoBAC 5; Rye

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HORDEUM; HORDEUM VULGARE SUBSP. VULGARE; LUPINUS; PHALARIS; PHALARIS COERULESCENS; SECALE CEREALE;

ACETIC ACID DERIVATIVE; ETHYLENEDIAMINE DERIVATIVE; PLANT DNA; TRIS ACETATE EDTA BUFFER; TRIS-ACETATE-EDTA BUFFER;

ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BARLEY; ESCHERICHIA COLI; GENE LIBRARY; GENE VECTOR; GENETICS; GENOMICS; ISOLATION AND PURIFICATION; LUPIN; METHODOLOGY; PHALARIS; PLANT; PULSED FIELD GEL ELECTROPHORESIS; RYE;

ACETATES; CHROMOSOMES, ARTIFICIAL, BACTERIAL; DNA, PLANT; ELECTROPHORESIS, GEL, PULSED-FIELD; ESCHERICHIA COLI; ETHYLENEDIAMINES; GENE LIBRARY; GENETIC VECTORS; GENOMICS; HORDEUM; LUPINUS; PHALARIS; PLANTS; SECALE CEREALE;

EID: 65349171478     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-427-8_4     Document Type: Article

References (14)

1. Collins, J. and Hohn, B. (1978) Cosmids: a type of plasmid gene-cloning vector that ispackagea-ble in vitro in bacteriophage lambda heads. Proc. Natl. Acad. Sci. USA 75, 4242-4246. (Pubitemid 9025986)
2. Burke, D.T., Carle, G.F., and Olson, M.V. (1987) Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236, 806-812. (Pubitemid 17071153)
3. Sternberg, N. (1990) Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs. Proc. Natl. Acad. Sci. USA 87, 103-107.
4. Shizuya, H., Birren, B., Kim, U.-J., et al. (1992) Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proc. Natl. Acad. Sci. USA 89, 8794-8797.
5. Zhang, H.B., Woo, S.S., and Wing, R.A. (1996) BAC, YAC and Cosmid Library Construction, in Plant Gene Isolation (Foster, G. and Twell, D., eds.), Wiley, New York, pp. 75-99.
6. Ioannou, P.A., Amemiya, C.T., Garnes, J., Kroisel, P.M., Shizuya, H., Chen, C., Batzer, M. A., and De Jong, P.J. (1994) A new bac-teriophage P1-derived vector for the propagation of large human DNA fragments. Nat. Genet. 6, 84-89. (Pubitemid 24191918)
7. Kelley, J.M., Field, C.E., Craven, M.B., Boc-skai, D., Kim, U.-J., Rounsley, S.D., and Adams, M.D. (1999) High throughput direct end sequencing of BAC clones. Nucleic Acids Res. 27, 1539-1546. (Pubitemid 29206298)
8. Shi, B.J., Collins, N., Miftahudin, B., Lan-gridge, P., and Gustafson, P. (2006) Construction of a rye cv. Blanco BAC library, and progress towards cloning the rye Alt3 aluminium tolerance gene. Vortr. Pflanzenzuchtg. 71, 205-209.
9. Ma, X.F., Wanous, M.K., Houchins, K., Rodriguez, Milla, M.A., Goicoehea, P.G., Wang, Z., Xie, M., and Gustafson, J.P. (2001) Molecular linkage mapping in rye (Secale cereale L.). Theor. Appl. Genet. 102, 517-523. (Pubitemid 32235408)
10. Amemiya, C.T., Ota, T., and Litman, G.W. (1996) Nonmammalian Genomic Analysis: A Practical Guide (Lai, E. and Birren, B., eds.) Academic Press, San Diego, CA, pp. 223-256
11. Birren, B., Green, E.D., Klapholz, S., Myers, R.M., and Roskams, J. (eds.) (1997) Analyzing DNA. CSH Laboratory Press, Cold Spring Harbor, New York.
12. Osoegawa, K., Woon, P. Y., Zhao, B., et al. (1998) An improved approach for construction of bacterial artificial chromosome libraries. Genomics 52, 1-8. (Pubitemid 28452164)
13. Choi, S. and Wing, R.A. (2000) Plant Molecular Biology Manual, 2nd ed. (Gelvin, S. and Schilperoort, R., eds.), Kluwer Academic Publishers, Norwell, MA, pp. 1-28. 14.
14. Peterson, D.G., Tomkins, J.P., Frisch, D.A., Wing, R.A., and Paterson, A.H. (2000) Construction of plant bacterial artificial chromosome (BAC) libraries: an illustrated guide. J. Agric. Genomics 5 (Beavis, B. and May, G., eds).