An isocratic fluorescence HPLC assay for the monitoring of L-asparaginase activity and L-asparagine depletion in children receiving E. coli L-asparaginase for the treatment of acute lymphoblastic leukaemia

Biomedical Chromatography

Volumn 23, Issue 2, 2009, Pages 152-159

  Nath, Christa E ^a   Dallapozza, Luciano ^a   Eslick, Adam E ^a   Misra, Ashish ^a   Carr, Deborah ^a   Earl, John W ^a  

^a CHILDREN S HOSPITAL AT WESTMEAD   (Australia)

Author keywords

HPLC; L asparaginase activity; L asparagine depletion

Indexed keywords

AMINO ACIDS; COLUMN CHROMATOGRAPHY; POTASSIUM COMPOUNDS; VOLATILE FATTY ACIDS;

ACUTE LYMPHOBLASTIC LEUKAEMIAS; E. COLI; HOMOSERINE; HPLC; ISOCRATIC; L ASPARAGINES; L-ASPARAGINASE; L-ASPARAGINASE ACTIVITY; L-ASPARAGINE DEPLETION; L-ASPARTIC ACID;

ESCHERICHIA COLI;

ASPARAGINASE; ASPARAGINE; ASPARTIC ACID; HOMOSERINE;

ACUTE LYMPHOBLASTIC LEUKEMIA; ADOLESCENT; ARTICLE; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; DERIVATIZATION; DRUG BLOOD LEVEL; DRUG RETENTION; DRUG STRUCTURE; ENZYME ACTIVITY; ESCHERICHIA COLI; FLOW RATE; FLUORESCENCE ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; PH MEASUREMENT; PROTEIN DEPLETION; STRUCTURE ANALYSIS;

EID: 65349158399     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.1096     Document Type: Article

References (17)

1. Asselin BH, Ryan D, Frantz CN, Bernal SD, Leavitt P, Sallan SE and Cohen HJ. In vitro and in vivo killing of acute lymphoblastic leukaemia cells by L-asparaginase. Cancer Research 1989; 49: 4363-4368.
2. Asselin BL, Lorenson MY, Whitin JC. Coppola DJ, Kende AS, Blakley RL and Cohen HJ. Measurement of serum L-asparagine in the presence of L-asparaginase requires the presence of an L-asparaginase inhibitor. Cancer Research 1991; 51: 6568-6573.
3. Asselin B, Gelber R and Sallan S. Relative toxicity of E. coli L-asparaginase (Asp) and PEGasparaginase (PEG) in newly diagnosed childhood acute lymphoblastic leukaemia (ALL). Medical and Pediatric Oncology 1993a; 21: 556.
4. Asselin BL, Whitin JC, Coppola DJ, Rupp IP, Sallan SE and Cohen HJ. Comparitive pharmacokinetic studies of three asparaginase preparations. Journal of Clinical Oncology 1993b; 11: 1780-1786.
5. Boos J, Werber E, Ahlke E, Schulze-Westhoff P, Nowak-Gottl U, Wurthwein G, Verspohl EJ, Ritter J and Jurgens H. Monitoring of asparaginase activity and asparagine levels in children on different asparaginase preparations. European Journal of Cancer 1996; 32A: 1544-1550.
6. Cooney D and Handschumacher R. L-Asparaginase and L-asparagine metabolism. Annual Review of Pharmacology 1970; 10: 421-440.
7. de Jonge L and Breuer M. Evaluation of systematic error due to deproteinization, calibration and storage of plasma for amino acid analysis by ion-exchange chromatography. Journal of Chromatography B 1996; 677: 61-68.
8. Gentili D, Zucchetti M, Conter V, Masera G and Incalci M. Determination of L-asparagine in biological samples in the presence of L-asparaginase. Journal of Chromatography B 1994; 657: 47-52.
9. Goldsmith RF, Earl JW and Cunningham AM. Determination of δ-aminobutyric acid and other amino acids in cerebrospinal fluid of paediatric patients by reversed phase liquid chromatography. Clinical Chemistry 1987; 33: 1736-1740.
10. Meister A. Methods in Enzymology II. Academic Press: New York, 1955; 380-385.
11. Miller HK, Salser JS and Balis ME. Amino acid levels following L-asparagine aminohydrolase (EC.3.5.1.1.) therapy. Cancer Research 1969; 29: 183-187.
12. Müller HJ and Boos J. Use of L-asparaginase in childhood ALL. Critical Reviews in Oncology/Hematology 1998; 28: 97-113.
13. Müller H-J, Beier R, Lönig L, Blütters-Sawatzki R, Dörffel W, Maas E, Müller-Weihrich S, Scheel-Walter H-G, Scherer F, Stahnke K, Schrappe M, Horn A, Lümkemann K and Boos J. Pharmacokinetics of native Escherichia coli asparaginase (Asparaginase medac) and hypersensitivity reactions in ALL-BFM 95 reinduction treatment. British Journal of Haematology 2001; 114: 794-799.
14. Panosyan EH, Seibel NL, Martin-Aragon S, Gaynon PS, Avramis IA, Sather H, Franklin J, Nachman J, Ettinger LJ, La M, Steinherz P, Cohen LJ, Siegel SE and Avramis VI. Asparaginase Antibody and asparaginase activity in children with higher risk acute lymphoblastic leukaemia. Journal of Pediatric Hematology Oncology 2004; 26: 217-226.
15. Reihm H, Gardner H, Henze G, Langerman HD, Odenwald E (1980) The Berlin childhood acute lymphoblastic leukemia therapy study, 1970-76. American Journal of Pediatric Hematology 2, 29-41.
16. Tagami S and Matsuda K. An enzymatic method for the kinetic measurement of L-asparaginase activity and L-asparagine with an ammonia gas sensing electrode. Chemical and Pharmaceutical Bulletin 1990; 38: 153-155.
17. Ylikangas P and Mononen I. A Fluorometric assay for L-asparaginase activity and monitoring of L-asparaginase therapy. Analytical Biochemistry 2000; 280: 42-45.