Detection of Bacillus sphaericus mosquitocidal toxin genes by multiplex colony PCR

Canadian Journal of Microbiology

Volumn 55, Issue 2, 2009, Pages 207-209

  Jagtap, Santosh C ^a   Jagtap, Chandrakant B ^a   Kumar, Pradeep ^a   Srivastava, R B ^b  

^a NAVAL MATERIALS RESEARCH LABORATORY   (India)

^b Defence R D Organization   (India)

Author keywords

Bacillus sphaericus; Btx bin and mtx toxin genes; Mosquito larvicidal; Multiplex colony pcr

Indexed keywords

AMPLICONS; BACILLUS SPHAERICUS; BTX/BIN AND MTX TOXIN GENES; GENES ENCODING; MOSQUITO LARVICIDAL; MULTIPLEX COLONY PCR; PCR ASSAYS; SINGLE TUBES;

BACTERIOLOGY; MULTIPLEXING;

GENES;

BACTERIAL TOXIN; PROTEIN BINA; PROTEIN BINB; PROTEIN MTX1; PROTEIN MTX2; UNCLASSIFIED DRUG;

BACTERIUM; BIOASSAY; DETECTION METHOD; POLYMERASE CHAIN REACTION; TOXIN;

AMPLICON; ARTICLE; BACILLUS SPHAERICUS; BACTERIUM CULTURE; BACTERIUM ISOLATE; CONTROLLED STUDY; GENE IDENTIFICATION; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL;

BACILLUS; BACTERIAL TOXINS; DNA PRIMERS; POLYMERASE CHAIN REACTION;

BACILLUS SPHAERICUS;

EID: 65349155160     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W08-113     Document Type: Article

References (7)

1. Baumann, P., Clark, M.A., Baumann, L., and Broadwell, A.H. 1991. Bacillus sphaericus as a mosquito pathogen: properties of the organism and its toxins. Microbiol. Rev. 55: 425-136. PMID:1682792.
2. Charles, J.-F., Nielsen-LeRoux, C., and Delecluse, A. 1996. Bacillus sphaericus toxins: molecular biology and mode of action. Annu. Rev. Entomol. 41: 451-172. doi:10.1146/annurev.en.41. 010196.002315. PMID:15012337.
3. Otsuki, K., Guaycurus, T.V., and Vicente, A.C.P. 1997. Bacillus sphaericus entomocidal potential determined by polymerase chain reaction. Mem. Inst. Oswaldo Cruz, 92: 107-108. doi:10. 1590/S0074- 02761997000100022.
4. Porter, A.G., Davidson, E.W., and Liu, J.-W. 1993. Mosquitocidal toxin of bacilli and their genetic manipulation for effective biological control of mosquitoes. Microbiol. Rev. 57: 838-861.PMID:7905597.
5. Shanmugavelu, M., Sritharan, V., and Jayaraman, K. 1995. Poly - merase chain reaction and nonradioactive gene probe based identification of mosquito larvicidal strains of Bacillus sphaeri - cus and monitering of B. sphaericus 1593M, released in the environment. J. Biotechnol. 39: 99-106. doi:10.1016/0168 - 1656(94)00145-3. PMID:7755972.
6. Thanabalu, T., and Porter, A.G. 1996. Bacillus sphaericus gene encoding a novel class of mosquitocidal toxin with homology to Clostridium and Pseudomonas toxin. Gene, 170: 85-89. doi:10. 1016/0378-1119(95)00836-5. PMID:8621095.
7. Thanabalu, T., Hindley, J., Jackson-Yap, J., and Berry, C. 1991. Cloning, sequencing, and expression of a gene encoding 100 - kilodalton mosquitolarvicidal toxin from Bacillus sphaericus SSII-1. J. Bacteriol. 173: 2776-2785. PMID:1840581.