Extending immunofluorescence detection limits in whole paraffin-embedded formalin fixed tissues using hyperspectral confocal fluorescence imaging

Journal of Microscopy

Volumn 234, Issue 2, 2009, Pages 137-146

  Constantinou, P ^a   Dacosta, R S ^a   Wilson, B C ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Autofluorescence; Confocal; Flavin; Fluorescence; Hyperspectral; Linear unmixing

Indexed keywords

CHEMICAL DETECTION; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; HISTOLOGY; IMAGE ACQUISITION; TISSUE;

AUTOFLUORESCENCE; CONFOCAL; EX-VIVO; FLAVIN; FLUORESCENT SIGNALS; HYPERSPECTRAL; LINEAR UNMIXING; SINGLE CHANNELS; SPECTRAL UNMIXING; VIVO TISSUE;

HYPERSPECTRAL IMAGING;

ANIMAL TISSUE; ARTICLE; AUTOFLUORESCENCE IMAGING; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FEMALE; IMMUNOFLUORESCENCE MICROSCOPY; MICROSCOPE IMAGE; MOUSE; NONHUMAN; PRIORITY JOURNAL; STAINING; TISSUE SECTION; TUMOR MODEL; TUMOR XENOGRAFT;

3,3'-DIAMINOBENZIDINE; ADENOCARCINOMA; ANIMALS; ANTIBODIES, NEOPLASM; CELL NUCLEUS; COLONIC NEOPLASMS; FLUORESCENT ANTIBODY TECHNIQUE; FORMALDEHYDE; HEMATOXYLIN; HUMANS; MICE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; NEOPLASM TRANSPLANTATION; PARAFFIN EMBEDDING; SENSITIVITY AND SPECIFICITY; TISSUE FIXATION;

EID: 65349123516     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2009.03155.x     Document Type: Article

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