A new affordable flow cytometry based method to measure HIV-1 viral load

Cytometry Part A

Volumn 75, Issue 3, 2009, Pages 199-206

  Greve, Burkhard ^a   Weidner, Jürgen ^b   Cassens, Uwe ^c   Odaibo, Georgina ^d   Olaleye, David ^d   Sibrowski, Walter ^a   Reichelt, Doris ^a   Nasdala, Ines ^b   Göhde, Wolfgang ^a  

^a UNIVERSITY HOSPITAL MÜNSTER   (Germany)

^b Partec GmbH   (Germany)

^c KLINIKUM DORTMUND   (Germany)

^d UNIVERSITY OF IBADAN   (Nigeria)

Author keywords

Flow cytometry; HIV; Viral load

Indexed keywords

2,4 DINITROPHENOL; BIOTIN; DIGOXYGENIN; PHYCOERYTHRIN; STREPTAVIDIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; COST BENEFIT ANALYSIS; DEVELOPED COUNTRY; DEVELOPING COUNTRY; FLOW CYTOMETRY; HUMAN IMMUNODEFICIENCY VIRUS 1; LONG TERMINAL REPEAT; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; VIRUS LOAD;

FLOW CYTOMETRY; HIV INFECTIONS; HIV-1; HUMANS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL LOAD;

EID: 65349117549     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20676     Document Type: Article

References (29)

1. Cassens U, Gohde W, Kuling G, Groning A, Schlenke P, Lehman LG, Traore Y, Servais J, Henin Y, Reichelt D, Greve B. Simplified volumetric flow cytometry allows feasible and accurate determination of CD4 T lymphocytes in immunodeficient patients worldwide. Antivir Ther 2004;9:395-405.
2. Greve B, Cassens U, Westerberg C, Gohde Jun W, Sibrowski W, Reichelt D, Gohde W. A new no-lyse, no-wash flow-cytometric method for the determination of CD4 T cells in blood samples. Transfus Med Hematother 2003;30:8-13.
3. O'Gorman MR, Zijenah LS. CD4 T cell measurements in the management of antire- troviral therapy-A review with an emphasis on pediatric HIV-infected patients. Cytometry Part B 2008;74B:19-26.
4. Bold A, Wurth R, Keller T, Trahorsch U, Voigt P, Schubert S, Sack U. Low-cost enumeration of CD4+ T cells using a density-based negative selection method (RosetteSep) for the monitoring of HIV-infected individuals in non-OECD countries. Cytometry Part A 2008;73A:28-35.
5. Greve B, Cassens U, Baumann C, Sibrowski W, Kuling G, Szabados F, Schlenke P, Reichelt D, Gebauer W, Gohde W. Determination of CD4 lymphocyte percentages using a new simplified and affordable no lyse flow cytometric protocol: A pilot study. J Lab Med (in press).
6. Ymeti A, Li X, Lunter B, Breukers C, Tibbe AG, Terstappen LW, Greve J. A single platform image cytometer for resource-poor settings to monitor disease progression in HIV infection. Cytometry Part A 2007;71A:132-142.
7. Rouet F, Rouzioux C. The measurement of HIV-1 viral load in resource-limited settings: How and where? Clin Lab 2007;53:135-148.
8. Drosten C, Panning M, Drexler JF, Hansel F, Pedroso C, Yeats J, de Souza Luna LK, Samuel M, Liedigk B, Lippert U, Sturmer M, Doerr HW, Brites C, Preiser W. Ultrasensitive monitoring of HIV-1 viral load by a low-cost real-time reverse transcrip- tion-PCR assay with internal control for the 5′ long terminal repeat domain. Clin Chem 2006;52:1258-1266.
9. Goicoechea M, Haubrich R. CD4 lymphoctye percentage versus absolute CD4 lymphocyte count in predicting HIV disease progression: An old debate revisited. J Infect Dis 2005;192:945-947.
10. Ivers LC, Kendrick D, Doucette K. Efficacy of antiretroviral therapy programs in resource-poor settings: A meta-analysis of the published literature. Clin Infect Dis 2005;41:217-224.
11. Kim JE, Beckthold B, Chen Z, Mihowich J, Malloch L, Gill MJ. Short communication: Identification of a novel HIV type 1 subtype H/J recombinant in Canada with discordant HIV viral load (RNA) values in three different commercial assays. AIDS Res Hum Retrovirus 2007;23:1309-1313.
12. Fiscus SA, Cheng B, Crowe SM, Demeter L, Jennings C, Miller V, Respess R, Stevens W. HIV-1 viral load assays for resource-limited settings. PLoS Med 2006;3:e417.
13. Abayomi EA, Landis RC. Flow cytometry as the spearhead for delivering sustainable and versatile laboratory services to HIV-burdened health care systems of the developing world: A Caribbean model. Cytometry Part B Clin Cytom 2008;74B:80-89.
14. Rouet F, Chaix ML, Nerrienet E, Ngo-Giang-Huong N, Plantier JC, Burgard M, Peeters M, Damond F, Ekouevi DK, Msellati P, Ferradini L, Rukobo S, Marechal V, Schrachsa N, Wakrim L, Rafalimanana C, Rakotoambinina B, Viard JP, Seigneurin JM, Rouzioux C. Impact of HIV-1 genetic diversity on plasma HIV-1 RNA quantification: Usefulness of the Agence Nationale de Recherches sur le SIDA second-generation long terminal repeat-based real-time reverse transcriptase polymerase chain reaction test. J Acquir Immune Defic Syndr 2007;45:380-388.
15. Cleland A, Davis C, Adams N, Lycett C, Jarvis LM, Holmes H, Simmonds P. Development of multiplexed nucleic acid testing for human immunodeficiency virus type 1 and hepatitis C virus. Vox Sang 2001;81:93-101.
16. Holmes H, Davis C, Heath A, Hewlett I, Lelie N. An international collaborative study to establish the 1st international standard for HIV-1 RNA for use in nucleic acid- based techniques. J Virol Methods 2001;92:141-150.
17. Somogiy S. Divergente Transkriptionskontrolle von HIV-1: Strukturelle und funktio- nale Analyse des LTR-Promotors der Gruppen M, N und O. Dissertation FU Berlin, 2002.
18. Chomczynski P, Mackey K. Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation. Anal Biochem 1995;225:163-164.
19. Hengen PN. Carriers for precipitating nucleic acids. Trends Biochem Sci 1996;21:224-225.
20. Pang J, Modlin J, Yolken R. Use of modified nucleotides and uracil-DNA glycosylase (UNG) for the control of contamination in the PCR-based amplification of RNA. Mol Cell Probes 1992;6:251-256.
21. Relucio K, Holodniy M. HIV-1 RNA and viral load. Clin Lab Med 2002;22:593- 610.
22. Berger A, Scherzed L, Sturmer M, Preiser W, Doerr HW, Rabenau HF. Comparative evaluation of the cobas amplicor HIV-1 monitor ultrasensitive test, the new cobas ampliprep/cobas amplicor HIV-1 monitor ultrasensitive test and the versant HIV RNA 3.0 assays for quantitation of HIV-1 RNA in plasma samples. J Clin Virol 2005;33:43-51.
23. Holodniy M. HIV-1 load quantitation: A 17-year perspective. J Infect Dis 2006;194 (Suppl 1):S38-S44.
24. Kantor R. Impact of HIV-1 pol diversity on drug resistance and its clinical implications. Curr Opin Infect Dis 2006;19:594-606.
25. Janossy G, Shapiro H. Overview: Simplified cytometry for routine monitoring of infectious diseases. Cytometry Part B Clin Cytom 2008;74B:6-10.
26. Zijenah LS, Kadzirange G, Madzime S, Borok M, Mudiwa C, Tobaiwa O, Mucheche M, Rusakaniko S, Katzenstein DA. Affordable flow cytometry for enumeration of absolute CD4+ T-lymphocytes to identify subtype C HIV-1 infected adults requiring antiretroviral therapy (ART) and monitoring response to ART in a resource-limited setting. J Transl Med 2006;4:33.
27. Wedemeyer N, Pascher E, Cassens U, Gohde W. Quantification of PCR products using microparticles and flow cytometry. Ann Biol Clin (Paris) 2004;62:65-71.
28. Nolan JP, Mandy F. Multiplexed and microparticle-based analyses: Quantitative tools for the large-scale analysis of biological systems. Cytometry Part A 2006;69A:318-325.
29. Council OoARA. Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents. Available at http://aidsinfo.nih.gov/ contentfiles/Adultand AdolescentGL.pdf2006.