EDTA Chelation therapy, without added vitamin C, decreases oxidative DNA damage and lipid peroxidation

Alternative Medicine Review

Volumn 14, Issue 1, 2009, Pages 56-61

  Roussel, Anne Marie ^a,b   Hininger Favier, Isabelle ^c   Waters, Robert S ^c   Osman, Mireille ^c   Fernholz, Karen ^c   Anderson, Richard A ^d  

^a UNIV GRENOBLE ALPES   (France)

^b Domaine Universitaire   (France)

^c Waters Preventive Medicine Center   (United States)

^d BELTSVILLE HUMAN NUTRITION RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BICARBONATE; CATALASE; DNA FORMAMIDOPYRIMIDINE GLYCOSYLASE; EDETIC ACID; GLUTATHIONE; HEPARIN; MAGNESIUM SULFATE; MALONALDEHYDE; PEROXIDE; POTASSIUM CHLORIDE; PROCAINE; PYRIDOXINE; SUPEROXIDE DISMUTASE; VITAMIN B COMPLEX;

ADULT; AGED; ANTIOXIDANT ACTIVITY; ARTICLE; BLOOD LEVEL; CHELATION THERAPY; CLINICAL ARTICLE; CONTROLLED STUDY; DNA DAMAGE; ENZYME ACTIVITY; ERYTHROCYTE; FEMALE; HUMAN; HUMAN CELL; LIPID PEROXIDATION; MALE; OXIDATIVE STRESS; PROTEIN BLOOD LEVEL; PROTEIN DEGRADATION; PROTEIN DETERMINATION;

AGED; ASCORBIC ACID; CHELATING AGENTS; DNA DAMAGE; EDETIC ACID; ERYTHROCYTES; FEMALE; HUMANS; LIPID PEROXIDATION; MALE; MALONDIALDEHYDE; MIDDLE AGED; OXIDATIVE STRESS; SUPEROXIDE DISMUTASE;

EID: 65349114196     PISSN: 10895159     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Hornig B, Drexler H. Reversal of endothelial dysfunction in humans. Coron Artery Dis 2001;12:463-473.
2. Vega-Lopez S, Devaraj S, Jialal I. Oxidative stress and antioxidant supplementation in the management of diabetic cardiovascular disease. J Investig Med 2004;52:24-32.
3. Pennathur S, Heinecke J W. Mechanisms of oxidative stress in diabetes: implications for the pathogenesis of vascular disease and antioxidant therapy. Front Biosci 2004;9:565-574.
4. Lamas GA, Ackermann A. Clinical evaluation of chelation therapy: is there any wheat amidst the chaffi? Am Heart J 2000;140:4-5.
5. Dandona P, Aljada A, Bandyopadhyay A. Inflammation: the link between insulin resistance, obesity and diabetes. Trends Immunol 2004;25:4-7.
6. Villarruz MV, Dans A, Tan F. Chelation therapy for atherosclerotic cardiovascular disease. Cochrane Database Syst Rev 2002;CD002785.
7. Anderson TJ, Hubacek J, Wyse DG, Knudtson ML. Effect of chelation therapy on endothelial function in patients with coronary artery disease: PATCH substudy. J Am Coll Cardiol 2003;41:420-425.
8. Seely DM, Wu P, Mills EJ. EDTA chelation therapy for cardiovascular disease: a systematic review. BMC Cardiovasc Disord 2005;5:32.
9. Hininger I, Waters R, Osman M, et al. Acute prooxidant effects of vitamin C in EDTA chelation therapy and long-term antioxidant benefits of therapy. Free Radic Biol Med 2005;38:1565-1570.
10. Sardi B. High-dose vitamin C and iron overload. Ann Intern Med 2004;140:846-847.
11. Anderson RA, Bryden NA, Waters R. EDTA chelation therapy does not selectively increase chromium losses. Biol Trace Elem Res 1999;70:265- 272.
12. Waters RS, Bryden NA, Patterson KY, et al. EDTA chelation effects on urinary losses of cadmium, calcium, chromium, cobalt, copper, lead, magnesium, and zinc. Biol Trace Elem Res 2001;83:207-221.
13. Waters RS, Fernholz K, Bryden NA, Anderson RA. Intravenous magnesium sulfate with and without EDTA as a magnesium load test - is magnesium deficiency widespread? Biol Trace Elem Res 2008;124:243-250.
14. Rozema TC. The protocol for safe and effective administration of EDTA and other chelating agents for vascular disease, degenerative disease and metal toxicity. J Advan Med 1997;10:11-17.
15. Hininger I, Chollat-Namy A, Sauvaigo S, et al. Assessment of DNA damage by comet assay on frozen total blood: method and evaluation in smokers and non-smokers. Mutat Res 2004;558:75-80.
16. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469-474.
17. Gunzler WA, Kremers H, Flohe L. An improved coupled test procedure for glutathione peroxidase (EC 1-11-1-9) in blood. Z Klin Chem Klin Biochem 1974;12:444-448.
18. Beers RF Jr, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase.J Biol Chem 1952;195:133-140.
19. Akerboom TP, Sies H. Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol 1981;77:373-382.
20. Faure P, Lafond JL. Measurement of plasma sulfhydryl and carbonyl groups as a possible indicator of protein oxydation. In: Favier AE, Cadet J, Kalnyanaraman M, et al, eds. Analysis of Free Radicals in Biological Systems. Basel, Switzerland: Birkäuser; 1995:237-248.
21. Richard MJ, Guiraud P, Meo J, Favier A. High- performance liquid chromatographic separation of malondialdehyde-thiobarbituric acid adduct in biological materials (plasma and human cells) using a commercially available reagent. J Chromatogr 1992;577:9-18.
22. Singh NP, McCoy MX Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184-191.
23. Pouget JP, Ravanat JL, Douki T, et al. Measurement of DNA base damage in cells exposed to low doses of gamma-radiation: comparison between the HPLC- EC and comet assays. Int J Radiat Biol 1999;75:51- 58.
24. Martinez-Alfaro M, Palma-Xirado L, Sandoval- Zapata F Carabez-Xrejo A. Correlation between formamidopyrimidine DNA glycosylase (Fpg)- sensitive sites determined by a comet assay, increased MDA, and decreased glutathione during long exposure to thinner inhalation. Toxicol Lett 2006;163:198-205.
25. Moller P. Assessment of reference values for DNA damage detected by the comet assay in human blood cell DNA. Mutat Res 2006;612:84-104.
26. Wu G, Fang YZ, Yang S, et al. Glutathione metabolism and its implications for health.J Nutr 2004;134:489-492.
27. Dincer Y, Akcay X, Alademir Z, Ilkova H. Assessment of DNA base oxidation and glutathione level in patients with type 2 diabetes. Mutat Res 2002;505:75-81.
28. Baynes J W. Role of oxidative stress in development of complications in diabetes. Diabetes 1991;40:405-412.
29. Green DJ, O'Driscoll JG, Maiorana A, et al. Effects of chelation with EDXA and vitamin B therapy on nitric oxide-related endothelial vasodilator function. Clin Exp Pharmacol Physiol 1999;26:853-856.
30. Duarte XL, Lunec J. Review: when is an antioxidant not an antioxidant? A review of novel actions and reactions of vitamin C. Free Radic Res 2005;39:671- 686.
31. Sakagami H, Satoh K, Modulating factors of radical intensity and cytotoxic activity of ascorbate (review). Anticancer Res 1997;17:3513-3520.
32. Kang JS, Cho D, Kim YI, et al. L-ascorbic acid (vitamin C) induces the apoptosis of B16 murine melanoma cells via a caspase-8-independent pathway. Cancer Immunol Immunother 2003;52:693-698.
33. Schwartz JL. The dual roles of nutrients as antioxidants and prooxidants: their effects on tumor cell growth. J Nutr 1996;126:1221S-1227S.