Purification and molecular cloning of xylanases from the wood-feeding termite, coptotermes formosanus shiraki

Bioscience, Biotechnology and Biochemistry

Volumn 73, Issue 3, 2009, Pages 710-718

  Arakawa, Gaku ^a   Watanabe, Hirofumi ^b   Yamasaki, Hideo ^a   Maekawa, Hideaki ^a   Tokuda, Gaku ^a  

^a UNIVERSITY OF THE RYUKYUS   (Japan)

^b NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

GHF11; Protist; Termite; Xylan; Xylanase

Indexed keywords

GHF11; PROTIST; TERMITE; XYLAN; XYLANASE;

AMINES; AMINO ACIDS; BAST FIBERS; CLONING; ENZYMATIC HYDROLYSIS; HYDROLYSIS; ORGANIC ACIDS; PLANT CELL CULTURE; POLYSACCHARIDES; PURIFICATION; TRANSCRIPTION;

CELLULOSE;

COMPLEMENTARY DNA; XYLAN 1,4 BETA XYLOSIDASE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CHEMISTRY; CHROMATOGRAPHY; ENZYMOLOGY; GASTROINTESTINAL TRACT; GENETICS; ISOLATION AND PURIFICATION; ISOPTERA; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; SYMBIOSIS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHROMATOGRAPHY; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GASTROINTESTINAL TRACT; ISOPTERA; MOLECULAR SEQUENCE DATA; NUCLEIC ACID AMPLIFICATION TECHNIQUES; SYMBIOSIS; XYLOSIDASES;

AMINES; AMINO ACIDS; ENZYMOLYSIS; HEMICELLULOSES; NUCLEIC ACIDS; POLYSACCHARIDES; PURIFICATION; TERMITES; WOOD; XYLANASE;

COPTOTERMES FORMOSANUS; HOLOMASTIGOTOIDES MIRABILE; ISOPTERA; MASTIGOPHORA (FLAGELLATES); PROTISTA; PROTOZOA;

EID: 65349098768     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.80788     Document Type: Article

References (40)

1. Ohkuma, M., Termite symbiotic systems: efficient bio-recycling of lignocellulose. Appl. Microbiol. Biotechnol., 61, 1-9 (2003).
2. Breznak, J. A., and Brune, A., Role of microorganisms in the digestion of lignocellulose by termites. Annu. Rev. Entomol., 39, 453-487 (1994).
3. Nakashima, K., Watanabe, H., Saitoh, H., Tokuda, G., and Azuma, J., Dual cellulose-digesting system of the wood-feeding termite, Coptotermes formosanus Shiraki. Insect Biochem. Mol. Biol., 32, 777-784 (2002).
4. Tokuda, G., Lo, N., Watanabe, H., Arakawa, G., Matsumoto, T., and Noda, H., Major alteration of the expression site of endogenous cellulases in members of an apical termite lineage. Mol. Ecol, 13, 3219-3228 (2004).
5. Tokuda, G., Lo, N., and Watanabe, H., Marked variations in patterns of cellulase activity against crystalline- vs. carbox-ymethyl-cellulose in the digestive systems of diverse, wood-feeding termites. Physiol. Entomol., 30, 372-380 (2005).
6. Tokuda, G., and Watanabe, H., Hidden cellulases in termites: revision of an old hypothesis. Biol. Lett., 3, 336-339 (2007).
7. Warnecke, F., Luginbühl, P., Ivanova, N., Ghassemian, M., Richardson, T. H., Stege, J. T., Cayouette, M., McHardy, A. C, Djordjevic, G., Aboushadi, N., Sorek, R., Tringe, S. G., Podar, M., Martin, H. G., Kunin, V., Dalevi, D., Madejska, J., Kirton, E., Platt, D., Szeto, E., Salamov, A., Barry, K., Mikhailova, N., Kyfides, N. C, Matson, E. G., Ottesen, E. A., Zhang, X., Hernández, M., Murillo, C, Acosta, L. G., Rigoutsos, I., Tamayo, G., Green, B. D., Chang, C, Rubin, E. M., Mathur, E. J., Robertson, D. E., Hugenholtz, P., and Leadbetter, J. R., Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite. Nature, 450, 560-565 (2007).
8. Yang, B., and Wyman, C. E., Effect of xylan and lignin removal by batch and flow-through pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnol. Bioeng., 86, 88-95 (2004).
9. Ohgren, K., Bura, R., Saddler, J., and Zacchi, G., Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover. Bioresour. Technol., 98, 2503-2510 (2007).
10. Polizeli, M. L. T. M., Rizzatti, A. C. S., Monti, R., Terenzi, H., Jorge, J., and Amorim, D., Xylanases from fungi: properties and industrial applications. Appl. Microbiol. Biotechnol., 67, 577-591 (2005).
11. Wong, K. K. Y., Tan, L. U. L., and Saddler, J. N., Multiplicity of beta-1,4-xylanase in microorganisms: functions and applications. Microbiol. Rev., 52, 305-317 (1988).
12. Inoue, T., Murashima, K., Azuma, J., Sugimoto, A., and Slaytor, M., Cellulose and xylan utilisation in the lower termite Reticulitermes speratus. J. Insect Physiol., 43, 235-242 (1997).
13. Todaka, N., Moriya, S., Saita, K., Hondo, T., Kiuchi, I., Takasu, H., Ohkuma, M., Piero, C, Hayashizaki, Y., and Kudo, T., Environmental cDNA analysis of the genes involved in lignocellulose digestion in the symbiotic protist community of Reticulitermes speratus. FEMS Microbiol. Ecol., 59, 592-599 (2007).
14. Yoshimura, T., Azuma, J., Tsunoda, K., and Takahashi, M., Cellulose metabolism of the symbiotic protozoa in termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). I. Mokuzai Gakkaishi, 39, 221-226 (1993).
15. Yoshimura, T., Contribution of the protozoan fauna to nutritional physiology of the lower termite Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). Wood Res., 82, 68-129 (1995).
16. Watanabe, H., Nakashima, K., Saito, H., and Slaytor, M., New endo-beta-l,4-glucanases from the parabasalian symbionts, Pseudotrichonympha grassii and Holomastigotoides mirabile of Coptotermes termites. CMLS, 59, 1983-1992 (2002).
17. Nakashima, K. I., Watanabe, H., and Azuma, J. I., Cellulase genes from the parabasalian symbiont Pseudotrichonympha grassii in the hindgut of the wood-feeding termite Coptotermes formosanus. CMLS, 59, 1554-1560 (2002).
18. Inoue, T., Moriya, S., Ohkuma, M., and Kudo, T., Molecular cloning and characterization of a cellulase gene from a symbiotic protist of the lower termite, Coptotermes formosanus. Gene, 349, 67-75 (2005).
19. Tanaka, H., Aoyagi, H., Shina, S., Dodo, Y., Yoshimura, T., Nakamura, R., and Uchiyama, H., Influence of the diet components on the symbiotic microorganisms community in hindgut of Coptotermes formosanus Shiraki. Appl. Microbiol. Biotechnol., 71, 907-917 (2006).
20. Azuma, J., Nishimoto, K., and Koshijima, T., Studies on digestive system of termites. II. Properties of carbohydrolases of termite Coptotermes formosanus Shiraki. Wood Res., 70, 1-16 (1984).
21. Jue, C. K., and Lipke, P. N., Determination of reducing sugars in the nanomole range with tetrazolium blue. J. Biochem. Biophys. Methods, 11, 109-115 (1985).
22. Layne, E., Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol., 3, 447-454 (1957).
23. Sugimura, M., Watanabe, H., Lo, N., and Saito, H., Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris. Eur. J. Biochem., 270, 3455-3460 (2003).
24. Tokuda, G., Saito, H., and Watanabe, H., A digestive beta-glucosidase from the salivary glands of the termite, Neotermes koshunensis (Shiraki): distribution, characterization and isolation of its precursor cDNA by 5'- and 3'-RACE amplifications with degenerate primers. Insect Biochem. Mol. Biol., 32, 1681-1689 (2002).
25. Tokuda, G., Lo, N., Watanabe, H., Slaytor, M., Matsumoto, T., and Noda, H., Metazoan cellulase genes from termites: intron/ exon structures and sites of expression. Biochim. Biophys. Acta, 1447, 146-159 (1999).
26. Lo, N., Tokuda, G., Watanabe, H., Rose, H., Slaytor, M., Maekawa, K., Bandi, C, and Noda, H., Evidence from multiple gene sequences indicates that termites evolved from wood-feeding cockroaches. Curr. Biol., 10, 801-804 (2000).
27. Trager, W., The cultivation of a cellulose-digesting flagellate Trichomonas termopsidis and of certain other termite protozoa. Biol. Bull., 66, 182-190 (1934).
28. Henrissat, B., A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J., 280, 309-316 (1991).
29. Henrissat, B., and Bairoch, A., New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J., 293, 781-788 (1993).
30. Henrissat, B., and Bairoch, A., Updating the sequence-based classification of glycosyl hydrolases. Biochem. J., 316, 695-696 (1996).
31. Noirot, C, and Noirot-Timotheé, C, Proctodeal cuticle of insects. I. Comparative ultrastructure. Z. Zellforsch. Mikrosk. Anat, 101, 477-509 (1969).
32. Breznak, J. A., and Pankratz, H. S., In situ morphology of the gut microbiota of wood-eating termites [Reticulitermes flavipes (Kollar) and Coptotermes formosanus Shiraki]. Appl. Environ. Microbiol, 33, 406-426 (1977).
33. Noirot, C, and Noirot-Timotheé, C, The digestive system. In "Biology of Termites," Vol. 1, eds. Krishna, K., and Weesner, F., Academic Press, New York, pp. 49-88 (1969).
34. Potts, R. C, and Hewitt, P. H., Some properties and reaction characteristics of the partially purified cellulase from the termite Trinervitermes trinerboides (Nasutitermitinae). Comp. Biochem. Physiol, 47B, 327-337 (1974).
35. von Heijne, G., Towards a comparative anatomy of N-terminal topogenic protein sequences. J. Mol. Biol., 189, 239-242 (1986).
36. Yamaoka, I., and Nagatani, Y., Cellulose digestion system in the termite, Reticuütermes speratus (Kolbe). II. Ultrastructural changes related to the ingestion and digestion of cellulose by the flagellate, Trichonympha agilis. Zool. Mag. (in Japanese), 86, 34-43 (1977).
37. Ohkuma, M., Symbioses of flagellates and prokaryotes in the gut of lower termites. Trends Microbiol., 16, 345-352 (2008).
38. Geib, S. M., Filley, T. R., Hatcher, P. G., Hoover, K., Carlson, J. E., Jimenez-Gasco, M. D., Nakagawa-Izumi, A., Sleighter, R. L., and Tien, M., Lignin degradation in wood-feeding insects. PNAS, 105, 12932-12937 (2008).
39. Kyou, K., Watanabe, T., Yoshimura, T., and Takahashi, M., Lignin modification by termite and its symbiotic protozoa. Wood Res., 83, 50-54 (1996).
40. Ohkuma, M., Saita, K., Inoue, T., and Kudo, T., Comparison of four protein phylogeny of parabasalian symbionts in termite guts. Mol. Phylogenet. Evoi, 42, 847-853 (2007).