Rrp2, a σ 54-dependent transcriptional activator of Borrelia burgdorferi, activates rpoS in an enhancer-independent manner

Journal of Bacteriology

Volumn 191, Issue 8, 2009, Pages 2902-2905

  Blevins, Jon S ^a,b   Xu, Haijun ^c   He, Ming ^c   Norgard, Michael V ^b   Reitzer, Larry ^d   Yang, X Frank ^c,e  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^c INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF TEXAS AT DALLAS   (United States)

^e INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; RRP2 PROTEIN; SIGMA FACTOR RPOS; UNCLASSIFIED DRUG; UPSTREAM STIMULATORY FACTOR; BACTERIAL DNA; SIGMA FACTOR; SIGMA FACTOR KATF PROTEIN, BACTERIA; SIGMA FACTOR RPON; TRANSACTIVATOR PROTEIN;

ARTICLE; BACTERIAL GENE; BORRELIA BURGDORFERI; DNA SEQUENCE; LYME DISEASE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DETERMINATION; PROTEIN DNA BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; TRANSCRIPTION INITIATION; BIOSYNTHESIS; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; PHYSIOLOGY; PROTEIN BINDING;

BORRELIA BURGDORFERI;

BACTERIAL PROTEINS; BORRELIA BURGDORFERI; DNA, BACTERIAL; ELECTROPHORETIC MOBILITY SHIFT ASSAY; GENE EXPRESSION REGULATION, BACTERIAL; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; RNA POLYMERASE SIGMA 54; SIGMA FACTOR; TRANS-ACTIVATORS;

EID: 65249171323     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01721-08     Document Type: Article

References (27)

1. Beck, L. L., T. G. Smith, and T. R. Hoover. 2007. Look, no hands! Unconventional transcriptional activators in bacteria. Trends Microbiol. 15:530-537.
2. Blevins, J., K. E. Hagman, and M. V. Norgard. 2008. Assessment of decorin-binding protein A to the infectivity of Borrelia burgdorferi in the murine models of needle and tick infection. BMC Microbiol. 8:82.
3. Boardman, B. K., M. He, Z. Ouyang, H. Xu, X. Pang, and X. F. Yang. 2008. Essential role of the response regulator Rrp2 in the infectious cycle of Borrelia burgdorferi. Infect. Immun. 76:3844-3853.
4. 54-RNA polymerase holoenzyme. J. Bacteriol. 186:4535-4542.
5. Burtnick, M. N., J. S. Downey, P. J. Brett, J. A. Boylan, J. G. Frye, T. R. Hoover, and F. C. Gherardini. 2007. Insights into the complex regulation of rpoS in Borrelia burgdorferi. Mol. Microbiol. 65:277-293.
6. Caimano, M. J., R. Iyer, C. H. Eggers, C. Gonzalez, E. A. Morton, M. A. Gilbert, I. Schwartz, and J. D. Radolf. 2007. Analysis of the RpoS regulon in Borrelia burgdorferi in response to mammalian host signals provides insight into RpoS function during the enzootic cycle. Mol. Microbiol. 65:1193-1217.
7. Chen, P., and L. J. Reitzer. 1995. Active contribution of two domains to cooperative DNA binding of the enhancer-binding protein nitrogen regulator I (NtrC) of Escherichia coli: stimulation by phosphorylation and the binding of ATP. J. Bacteriol. 177:2490-2496.
8. 54 is required for mammalian infection and vector transmission but not for tick colonization. Proc. Natl. Acad. Sci. USA 102:5162-5167.
9. Fraser, C. M., S. Casjens, W. M. Huang, G. G. Sutton, R. Clayton, R. Lathigra, O. White, K. A. Ketchum, R. Dodson, E. K. Hickey, M. Gwinn, B. Dougherty, J.-F. Tomb, R. D. Fleischmann, D. Richardson, J. Peterson, A. R. Kerlavage, J. Quackenbush, S. Salzberg, M. Hanson, R. van Vugt, N. Palmer, M. D. Adams, J. Gocayne, J. Weidman, T. Utterback, L. Watthey, L. McDonald, P. Artiach, C. Bowman, S. Garland, C. Fujii, M. D. Cotton, K. Horst, K. Roberts, B. Hatch, H. O. Smith, and J. C. Venter. 1997. Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi. Nature 390: 580-586.
10. Hubner, A., X. Yang, D. M. Nolen, T. G. Popova, F. C. Cabello, and M. V. Norgard. 2001. Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway. Proc. Natl. Acad. Sci. USA 98:12724-12729.
11. Joslin, S. N., and D. R. Hendrixson. 2008. Analysis of the Campylobacter jejuni FlgR response regulator suggests integration of diverse mechanisms to activate an NtrC-Like protein. J. Bacteriol. 190:2422-2433.
12. Lybecker, M. C., and D. S. Samuels. 2007. Temperature-induced regulation of RpoS by a small RNA in Borrelia burgdorferi. Mol. Microbiol. 64:1075-1089.
13. 54). Mol. Microbiol. 10:903-909.
14. Ouyang, Z., J. S. Blevins, and M. V. Norgard. 2008. Transcriptional interplay among the regulators Rrp2, RpoN, and RpoS in Borrelia burgdorferi. Microbiology 154:2641-2658.
15. Popham, D. L., D. Szeto, J. Keener, and S. Kustu. 1989. Function of a bacterial activator protein that binds to transcriptional enhancers. Science 243:629-635.
16. Reed, L. J., and H. Muench. 1938. A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27:493-497.
17. Reitzer, L. J., and B. Magasanik. 1986. Transcription of glnA in E. coli is stimulated by activator bound to sites far from the promoter. Cell 45:785-792.
18. Revel, A. T., J. S. Blevins, C. Almazan, L. Neil, K. M. Kocan, J. de la Fuente, K. E. Hagman, and M. V. Norgard. 2005. bptA (bbe16) is essential for the persistence of the Lyme disease spirochete, Borrelia burgdorferi, in its natural tick vector. Proc. Natl. Acad. Sci. USA 102:6972-6977.
19. Rosa, P. A., K. Tilly, and P. E. Stewart. 2005. The burgeoning molecular genetics of the Lyme disease spirochaete. Nat. Rev. Microbiol. 3:129-143.
20. Singh, S. K., and H. J. Girschick. 2004. Molecular survival strategies of the Lyme disease spirochete Borrelia burgdorferi. Lancet Infect. Dis. 4:575-583.
21. N). J. Bacteriol. 189:2139-2144.
22. 54-dependent transcriptional activators. J. Bacteriol. 185:1757-1767.
23. Wedel, A., and S. Kustu. 1995. The bacterial enhancer-binding protein NtrC is a molecular machine: ATP hydrolysis is coupled to transcriptional activation. Genes Dev. 9:2042-2052.
24. Yan, D., H. S. Cho, C. A. Hastings, M. M. Igo, S. Y. Lee, J. G. Pelton, V. Stewart, D. E. Wemmer, and S. Kustu. 1999. Beryllofluoride mimics phos- phorylation of NtrC and other bacterial response regulators. Proc. Natl. Acad. Sci. USA 96:14789-14794.
25. Yang, X. F., S. M. Alani, and M. V. Norgard. 2003. The response regulator Rrp2 is essential for the expression of major membrane lipoproteins in Borrelia burgdorferi. Proc. Natl. Acad. Sci. USA 100:11001-11006.
26. Yang, X. F., Y. Ji, B. L. Schneider, and L. Reitzer. 2004. Phosphorylation-independent dimer-dimer interactions by the enhancer-binding activator NtrC of Escherichia coli: a third function for the C-terminal domain. J. Biol. Chem. 279:36708-36714.
27. Yang, X. F., U. Pal, S. M. Alani, E. Fikrig, and M. V. Norgard. 2004. Essential role for OspA/B in the life cycle of the Lyme disease spirochete. J. Exp. Med. 199:641-648.