DNA identification of biological traces and interpretation in a sexual assault case

American Journal of Forensic Medicine and Pathology

Volumn 30, Issue 1, 2009, Pages 57-60

  Apostolov, Aleksandar ^a   Hristov, Stanislav ^a   Angelova, Ekaterina ^a  

^a MEDICAL UNIVERSITY OF SOFIA   (Bulgaria)

Author keywords

DNA typing; Forensic casework; Interpretation; Sexual assault; STR

Indexed keywords

ADULT; ARTICLE; CASE REPORT; CLINICAL FEATURE; CONDOM; CYTOLOGY; DNA FINGERPRINTING; ELECTROPHORESIS; FEMALE; FORENSIC MEDICINE; GENE LOCUS; HUMAN; SEMEN ANALYSIS; SEXUAL CRIME; SPERM; GENE FREQUENCY; GENETIC MARKER; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; MALE; METHODOLOGY; POLYMERASE CHAIN REACTION; RAPE; SPERMATOZOON; TANDEM REPEAT; X CHROMOSOME; Y CHROMOSOME;

DNA;

ADULT; CHROMOSOMES, HUMAN, X; CHROMOSOMES, HUMAN, Y; CONDOMS; DNA; DNA FINGERPRINTING; FEMALE; GENE FREQUENCY; GENETIC MARKERS; HUMANS; MALE; POLYMERASE CHAIN REACTION; RAPE; SPECIMEN HANDLING; SPERMATOZOA; TANDEM REPEAT SEQUENCES;

EID: 65249133004     PISSN: 01957910     EISSN: None     Source Type: Journal    
DOI: 10.1097/PAF.0b013e31818737fd     Document Type: Article

References (22)

1. Drobnic K. Analysis of DNA evidence recovered from epithelial cells in penile swabs. Croat Med J. 2003; 44:350-354.
2. Sibille I, Duverneuil C, Lorin-de-la-Grandmaison G, et al. Y-STR DNA amplification as biological evidence in sexually assaulted female victims with no cytological detection of spermatozoa. Forensic Sci Int. 2002 Feb 18; 125: 212-216.
3. Cina MSJ, Collins KA, Fitts M, et al. Isolation and identification of male and female DNA on a postcoital condom. Arch Pathol Lab Med. 2000; 124:1083-1086.
4. Collins PJ, Rao PN, Hauworth R, et al. Identification of sperm and non-sperm male cells in cervicovaginal smears using fluorescence in situ hybridization: applications in alleged sexual assault cases. J Forensic Sci. 1994; 39:1347-1355.
5. Rao PN, Collins KA, Geisinger KR, et al. Identification of male epithelial cells in routine post-coital cervicovaginal smears using fluorescence in situ hybridization. Am J Clin Pathol. 1995; 104:32-35.
6. Short J, DeLuca M, DiVasto PV, et al. Detection of sperm in victims of rape. New Engl J Med. 1978; 299:424.
7. Quinn PJ. " Deoxyribonuclease Activity in Semen. " J Reprod Fertil. 1968; 17:35-39.
8. Sawazaki K, Yasuda T, Nadano D, et al. Deoxyribonuclease I (DNase I) typing from semen stains: low enzyme activity in vaginal fluids does not interfere with seminal dnase I typing from mixture stains. J Forensic Sci. 1993; 36:1051-1062.
9. Edwards AI, Civitello A, Hammond HA, et al. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet. 1991; 49:746-756.
10. Saiki RK, Gefland DH, Stoffel S, et al. Primer-directed enzymatic amplification of DNA with a termostabile DNA polymerase. Science. 1988; 239:487-491.
11. Sajantila A, Budowle B. Identification of individuals with DNA testing. Ann Med. 1991; 23:637-642.
12. GenePrint DNA Typing. Promega Corporation-Technical Manual; 1996.
13. Waye JS, Presley LA, Budowle B, et al. A simple and sensitive method for quantifying human genomic DNA in forensic specimen extracts. Biotechniques. 1989; 7:852-855.
14. Christov S, Apostolov A, Christov A, et al. DNA analysis of traces from biological material in cases of sexual assaults. Forensic science and criminality. 2002; (107-108):115-119.
15. Christov S, Christov A, Apostolov A, et al. Distribution of alleles on microsatelite markers Hum FES, Hum FIBRA, D18S51 in Bulgarian population. Forensic Sci criminality. 2002; (107-108):106-114.
16. Christov S, Apostolov A, Christov A, et al. DNA analysis of marks with human origin on evidences in forensic-medical examination. Modern medicine 2003; 13:10-15.
17. Urquhart A, Oldroyd NJ, Kimpton CP, et al. Highly discriminating heptaplex Short Tandem Repeat PCR System for Forensic Indentification. BioTechniques. 1995; 18:116-121.
18. Christov S, Apostolov A, Angelova E. DNA analysis of Y chromosome markers in post-coital assays examination. Praemedicus. 2003; XXII:149-154.
19. Hou Y, Zhang J, Sun D, et al. Typing Y chromosome STR haplotypes using redesigned primers. J Forensic Sci. 2002; 47:215-217.
20. Roewer L, Kayser M, Nagy MMale indentification using Y-chromosomal STR polymorphisms. In: Carracedo A, Brinkmann B, Bar W, eds. Advances in Forensic Haemogenetics 6. New York, NY: Springer-Verlag; 1996:124-126.
21. Zaharova B, Andonova S, Decorte R, et al. DNA Paternity testing in Bulgaria. Forensic Sci Int. 2003; 136:43.
22. Decorte R. Evaluation of the ALF DNA sequencer for high-speed sizing of short tandem repeat alleles. Electrophoresis. 1996; 17:1542-1549.