Imaging protein-protein interactions by förster resonance energy transfer (FRET) microscopy in live cells

Current Protocols in Protein Science

Volumn , Issue SUPPL. 56, 2009, Pages

  Brzostowski, Joseph A ^a   Meckel, Tobias ^a   Hong, Jiang ^a   Chen, Alice ^a   Jin, Tian ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Acceptor photobleaching; Cerulean; FRET; N FRET; Sensitized emission; Venus; Zeiss LSM 510

Indexed keywords

CYAN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; YELLOW FLUORESCENT PROTEIN;

BLEACHING; CELL CULTURE; CONFOCAL MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENETIC TRANSFECTION; HUMAN; MOLECULAR CLONING; MOLECULAR IMAGING; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; REVIEW; ANALYTIC METHOD; ARTICLE; CELL TRANSPORT; IMAGE ANALYSIS; IMAGE PROCESSING; LIVE CELL IMAGING; MICROSCOPY; PROCEDURES CONCERNING CELLS; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION;

CELL LINE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, CONFOCAL; PHOTOBLEACHING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN INTERACTION MAPPING; PROTEINS;

EID: 65249131675     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps1905s56     Document Type: Review

References (23)

1. Becker, W. 2005. Advanced Time-Correlated Single Photon Counting Techniques. Berlin, Springer.
2. Förster, T. 1949. Experimental and theoretical investigation of the intermolecular transfer of electronic excitation energy. Z. Naturforsch. A 4:7.
3. Giepmans, B.N., Adams, S.R., Ellisman, M.H., and Tsien, R.Y. 2006. The fluorescent toolbox for assessing protein location and function. Science 312:217-224.
4. Gordon, G.W., Berry, G., Liang, X.H., Levine, B., and Herman, B. 1998. Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy. Biophys. J. 74:2702- 2713.
5. Janetopoulos, C., Jin, T., and Devreotes, P. 2001. Receptor-mediated activation of heterotrimeric G-proteins in living cells. Science 291:2408- 2411.
6. Koushik, S.V., Chen, H., Thaler, C., Puhl, H.L., and Vogel, S.S. 2006. Cerulean, Venus, and VenusY67C FRET reference standards. Biophys. J. 91:L99-L101.
7. Lakowicz, J.R. 1999. Principles of Fluorescence Spectroscopy, 2nd ed. Kluwer Academic/Plenum Publishers, New York.
8. Lissandron, V., Terrin, A., Collini, M., D'alfonso, L., Chirico, G., Pantano, S., and Zaccolo, M. 2005. Improvement of a FRET-based indicator for cAMP by linker design and stabilization of donor-acceptor interaction. J. Mol. Biol. 354:546-555.
9. Miyawaki, A., Llopis, J., Heim, R., McCaffery, J.M., Adams, J.A., Ikura, M., and Tsien, R.Y. 1997. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388:882-887.
10. Nagai, Y., Miyazaki, M., Aoki, R., Zama, T., Inouye, S., Hirose, K., Iino, M., and Hagiwara, M. 2000. A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo. Nat. Biotechnol. 18:313-316.
11. Nagai, T., Ibata, K., Park, E.S., Kubota, M., Mikoshiba, K., and Miyawaki, A. 2002. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20:87-90
12. Periasamy, A. and Day, R.N. eds. 2005. Molecular Imaging: FRET Microscopy and Spectroscopy. Oxford, Oxford University Press.
13. Rizzo, M.A., Springer, G.H., Granada, B., and Piston, D.W. 2004. An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22:445-449.
14. Shaner,N.C., Steinbach, P.A., and Tsien,R.Y. 2005. A guide to choosing fluorescent proteins. Nat. Methods 2:905-909.
15. Sohn, H.W., Tolar, P., Jin, T., and Pierce, S.K. 2006. Fluorescence resonance energy transfer in living cells reveals dynamic membrane changes in the initiation of B cell signaling. Proc. Natl. Acad. Sci. U.S.A. 103:8143-8148.
16. Tolar, P., Sohn, H.W., and Pierce, S.K. 2005. The initiation of antigen-induced B cell antigen receptor signaling viewed in living cells by fluorescence resonance energy transfer. Nat. Immunol. 6:1168-1176.
17. van Rheenen, J., Langeslag, M., and Jalink, K. 2004. Correcting confocal acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized emission. Biophys. J. 86:2517-2529.
18. Wouters, F.S. and Bastiaens, P.I.H. 2001. Imaging protein-protein interactions by fluorescence energy transfer (FRET) microscopy. Curr. Protoc. Protein Sci. 23:19.5.1-19.5.15.
19. Xia, Z. and Liu, Y. 2001. Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes. Biophys. J. 81:2395-2402.
20. Xu, X., Meier-Schellersheim, M., Jiao, X., Nelson, L.E., and Jin, T. 2005. Quantitative imaging of single live cells reveals spatiotemporal dynamics of multistep signaling events of chemoattractant gradient sensing in Dictyostelium. Mol. Biol. Cell 16:676-688.
21. Xu, X., Brzostowski, J.A., and Jin, T. 2006. Using quantitative fluorescence microscopy and FRET imaging to measure spatiotemporal signaling events in single living cells. Methods Mol. Biol. 346:281-296.
22. Youvan, D.C., Silva, C.M., Bylina, E.J., Coleman, W.J., Dilworth, M.R., and Yang, M.M. 1997. Calibration of fluorescence resonance energy transfer in microscopy using genetically engineered GFP derivatives on nickel chelating beads. Biotechnology et alia 3:18.
23. Zal, T. and Gascoigne, N.R. 2004. Photobleachingcorrected FRET efficiency imaging of live cells. Biophys. J. 86:3923-3939.