Development of an in-capillary approach to nanoscale automated in vitro cytochromes P450 assays

Journal of Medicinal Chemistry

Volumn 52, Issue 8, 2009, Pages 2192-2195

  Nicoli, Raul ^a   Curcio, Raffaele ^a   Rudaz, Serge ^a   Veuthey, J L ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450; CYTOCHROME P450 2D6; CYTOCHROME P450 ISOENZYME; DRUG METABOLITE;

ARTICLE; AUTOANALYSIS; CAPILLARY; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DRUG METABOLISM; ENZYME ASSAY; ENZYME INHIBITION; ENZYME MECHANISM; ENZYME SUBSTRATE; HUMAN; IN VITRO STUDY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NANOANALYSIS; QUALITATIVE ANALYSIS; REPRODUCIBILITY; TECHNIQUE; ULTRAHIGH PRESSURE LIQUID CHROMATOGRAPHY;

AUTOANALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYCLOHEXANOLS; CYTOCHROME P-450 CYP2D6; CYTOCHROME P-450 ENZYME SYSTEM; DEXTROMETHORPHAN; FLUOXETINE; HUMANS; ISOENZYMES; MASS SPECTROMETRY; PHARMACEUTICAL PREPARATIONS; QUINIDINE; RECOMBINANT PROTEINS; SOLUTIONS;

EID: 65249098209     PISSN: 00222623     EISSN: None     Source Type: Journal    
DOI: 10.1021/jm900201b     Document Type: Article

References (25)

1. Lee, M.-Y.; Dordick, J. S. High-throughput human metabolism and toxicity analysis. Curr. Opin. Biotechnol. 2006, 17, 619-627.
2. Baranczewski, P.; Stanczak, A.; Sundberg, K.; Svensson, R.; Wallin, A.; Jansson, J.; Garberg, P.; Postlind, H. Introduction to in vitro estimation of metabolic stability and drug interactions of new chemical entities in drug discovery and development. Pharmacol. Rep. 2006, 58, 453-472.
3. Crespi, C. L.; Miller, V. P.; Penman, B. W. Microtiter plate assays for inhibition of human drug-metabolizing cytochromes P450. Anal. Biochem. 1997, 248, 188-190.
4. Cohen, L. H.; Remley, M. J.; Raunig, D.; Vaz, A. D. N. In vitro drug interactions of cytochrome P450: an evaluation of fluorogenic to conventional substrates. Dmg. Metgh. Dispos. 2003, 31, 1005-1015.
5. Clement Jerdi, M.; Daali, Y.; Oestreicher, M. K.; Cherkaoui, S.; Dayer, P. A simplified method for a phenotyping cocktail of major CYP450 biotransformation routes. J. Pharm. Biomed. Anal. 2004, 35, 1203- 1212.
6. Walsky, R. L.; Obach, R. S. Validated assays for human cytochrome P450 activities. Drug Metab. Dispos. 2004, 32, 647-660.
7. Donato, M. T.; Jimenez, N.; Castell, J. V.; Gomez-Lechon, M. J. Fluorescence-based assays for screening nine cytochrome P450 (P450) activities in intact cells expressing individual human P450 enzymes. Dru. Metah. Dispos. 2004, 32, 699-706.
8. Ghosal, A.; Hapangama, N.; Yuan, Y.; Lu, X.; Horne, D.; Patrick, J. E.; Zbaida, S. Rapid determination of enzyme activities of recombinant human cytochromes P450, human liver microsomes and hepatocytes. Biopharm. Drug. Mispos. 2003, 24, 375-384.
9. Chauret, N.; Tremblay, N.; Lackman, R. L.; Gauthier, J.-Y.; Silva, J. M.; Marois, J.; Yergey, J. A.; Nicoll-Griffith, D. A. Description of a 96-well plate assay to measure cytochrome P4503A inhibition in human liver microsomes using a selective fluorescent probe. Anal. Biochem. 1999, 276, 215-226.
10. Jenkins, K. M.; Angeles, R.; Quintos, M. T.; Xu, R.; Kassel, D. B.; Rourick, R. A. Automated high throughput ADME assays for metabolic stability and cytochrome P450 inhibition profiling of combinatorial libraries. J. Pharm. Biomed. Anal. 2004, 34, 989-1004.
11. Nicoli, R.; Bartolini, M.; Rudaz, S.; Andrisano, V.; Veuthey, J.-L. Development of immobilized enyzme reactors based on human recombinant cytochrome P450 enzymes for phse I drug metabolism studies. J. Chromatogr., A 2008, 1206, 2-10.
12. Bao, J.; Regnier, F. E. Ultramicro enzyme assays in a capillary electrophoretic system. J. Chromatogr. 1992, 608, 217-224.
13. Novakova, S.; Van Dick, S.; Van Schepdael, A.; Hoogmartens, J.; Glatz, Z. Electrophoretically mediated microanalysis. J. Chromatogr., A 2004, 1032, 173-184.
14. Zhang, J.; Hoogmartens, J.; Van Schepdael, A. Kinetic study of cytochrome P450 by capillary electrophoretically mediated microanalysis. Electrophoresis 2008, 29, 3694-3700.
15. Prakash, C.; Shaffer, C. L.; Nedderman, A. Analytical strategies for identifying drug metabolites. Mass Spectrom. Rev. 2007, 26, 340- 369.
16. Kostiainen, R.; Kotiaho, T.; Kuuranne, T.; Auriola, S. Liquid chromatography/atmospheric pressure ionization-mass spectrometry in drug metabolism studies. J. Mass Spectrom. 2003, 38, 357-372.
17. Rainville, P. D.; Wheaton, J. P.; Alden, P. G.; Plumb, R. S. Sub one minute inhibition assays for the major cytochrome P450 enzymes utilizing ultra-performance liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2008, 22, 1345-1350.
18. Yu, K.; Kerns, E.; Li, S. Q.; Alden, P.; Plumb, R. S. Ultra-performance liquid chromatography/tandem mass spectrometric quantification of structurally diverse drug mixtures using an ESI-APCI multimode ionization. Rapid Commun. Mass Spectrom. 2007, 21, 893-902.
19. Le Potier, I.; Taverna, M.; Fattal, E.; Benzaki, J.; Chevalier, M.; Ferrier, D. Performance evaluation of capillary surface treatments for peptide mapping by capillary zone electrophoresis. Chromatographia 2001, 53, 563-570.
20. Tiller, P. R.; Yu, S.; Castro-Perez, J.; Fillgrove, K. L.; Baille, T. A. High-throughput, accurate mass liquid chromatography/tandem mass spectrometry on a quadrupole time-of-flight system as a "first-line" approach for metabolite identification studies. Rapid Commun. Mass S'ectrom. 2008, 22, 1053-1061.
21. OConnor, D.; Mortishire-Smith, R.; Morrison, D.; Davies, A.; Dominguez, M. Ultra-performance liquid chromatography coupled to time-of-flight mass spectrometry for robust, high-throughput quantitative analysis of an automated metabolic stability assay, with simultaneous determination of metabolic data. RapidCommun. Mass Spectrom. 2006, 20, 851-857.
22. Guengerich, F. P. Human Cytochrome P450 Enzymes. In Cytochrome P450: Structure, Mechanism, and Biochemistry, 2nd ed.; Ortiz de Montellano, P. R., Ed.; Plenum Publishing Co.: New York, 1995; pp 473-535.
23. Mahgoub, A.; Idle, J. R.; Dring, L. G.; Lancaster, R.; Smith, R. L. Polymorphic hydroxylation of debrisoquine in man. Lmcet 1977, 2, 584-586.
24. Stevens, J. C; Wrighton, S. A. Interactions of the enantiomers of fluoxetine and norfluoxetine with human liver cytochromes P450. J. Pharmacol. Exp. Ther. 1993, 266, 964-971.
25. Otton, S. V.; Ball, S. E.; Cheung, S. W.; Inaba, T.; Rudolph, R. L.; Sellers, E. M. Venlafaxine oxidation in vitro is catalysed by CYP2D6. Br. J. Clin. Pharmacol. 1996, 41, 149-156. JM900201B