A radioisotope label-free α-bungarotoxin-binding assay using BIAcore sensor chip technology for real-time analysis

Analytical Biochemistry

Volumn 389, Issue 1, 2009, Pages 86-88

  Paulo, Joao A ^a   Hawrot, Edward ^b  

^a Graduate Program in Molecular Biology   (United States)

^b BROWN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; PROTEINS; SURFACE PLASMON RESONANCE;

ACETYLCHOLINE-BINDING PROTEINS; BINDING ASSAYS; BINDING INTERACTION; BINDING PROTEINS; BIOLOGICAL SAMPLES; INTERACTION STUDIES; NICOTINIC ACETYLCHOLINE RECEPTORS; REAL TIME ANALYSIS;

RADIOISOTOPES;

ACETYLCHOLINE; ALPHA BUNGAROTOXIN; BINDING PROTEIN; IODINE 125;

ARTICLE; BINDING ASSAY; CONTROLLED STUDY; ISOTOPE LABELING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SURFACE PLASMON RESONANCE; VALIDATION PROCESS;

EID: 65049086929     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.03.011     Document Type: Article

References (17)

1. Fivash M., Towler E.M., and Fisher R.J. BIAcore for macromolecular interaction. Curr. Opin. Biotechnol. 9 (1998) 97-101
2. Malmqvist M. BIACORE: an affinity biosensor system for characterization of biomolecular interactions. Biochem. Soc. Trans. 27 (1999) 335-340
3. Raghavan M., and Bjorkman P.J. BIAcore: a microchip-based system for analyzing the formation of macromolecular complexes. Structure 3 (1995) 331-333
4. Salvaterra P.M., and Mahler H.R. Nicotinic acetylcholine receptor from rat brain: solubilization, partial purification, and characterization. J. Biol. Chem. 251 (1976) 6327-6334
5. Moise L., Zeng H., Caffery P., Rogowski R.S., and Hawrot E. Structure and function of α-bungarotoxin. J. Toxicol. Toxin Rev. 21 (2002) 293-317
6. Dutertre S., and Lewis R.J. Toxin insights into nicotinic acetylcholine receptors. Biochem. Pharmacol. 72 (2006) 661-670
7. Sine S.M., and Engel A.G. Recent advances in Cys-loop receptor structure and function. Nature 440 (2006) 448-455
8. Brejc K., van Dijk W.J., Klaassen R.V., Schuurmans M., van der Oost J., Smit A.B., and Sixma T.K. Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors. Nature 411 (2001) 269-276
9. Johnsson B., Lofas S., and Lindquist G. Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors. Anal. Biochem. 198 (1991) 268-277
10. Smit A.B., Syed N.I., Schaap D., van Minnen J., Klumperman J., Kits K.S., Lodder H., van der Schors R.C., van Elk R., Sorgedrager B., Brejc K., Sixma T.K., and Geraerts W.P. A glia-derived acetylcholine-binding protein that modulates synaptic transmission. Nature 411 (2001) 261-268
11. Bracci L., Lozzi L., Lelli B., Pini A., and Neri P. Mimotopes of the nicotinic receptor binding site selected by a combinatorial peptide library. Biochemistry 40 (2001) 6611-6619
12. Spiga O., Bernini A., Scarselli M., Ciutti A., Bracci L., Lozzi L., Lelli B., Di Maro D., Calamandrei D., and Niccolai N. Peptide-protein interactions studied by surface plasmon and nuclear magnetic resonances. FEBS Lett. 511 (2002) 33-35
13. Wang H., and Pevsner J. Detection of endogenous biotin in various tissues: novel functions in the hippocampus and implications for its use in avidin-biotin technology. Cell Tissue Res. 296 (1999) 511-516
14. McKay B.E., Molineux M.L., and Turner R.W. Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques. Methods Mol. Biol. 418 (2008) 111-128
15. Edwards D.A. Steric hindrance effects on surface reactions: applications to BIAcore. J. Math. Biol. 55 (2007) 517-539
16. Nelson R.W., Nedelkov D., and Tubbs K.A. Biomolecular interaction analysis mass spectrometry: BIA/MS can detect and characterize proteins in complex biological fluids at the low to subfemtomole level. Anal. Chem. 72 (2000) 404A-411A
17. Nelson R.W., Nedelkov D., and Tubbs K.A. Biosensor chip mass spectrometry: a chip-based proteomics approach. Electrophoresis 21 (2000) 1155-1163