Phos-tag beads as an immunoblotting enhancer for selective detection of phosphoproteins in cell lysates

Analytical Biochemistry

Volumn 389, Issue 1, 2009, Pages 83-85

  Kinoshita Kikuta, Emiko ^a   Kinoshita, Eiji ^a   Koike, Tohru ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

IMMUNOBLOTTING; IMMUNOBLOTTING ANALYSIS; MITOGEN ACTIVATED PROTEIN KINASE; PRETREATMENT PROCEDURE; SELECTIVE DETECTION; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SPECIFIC DETECTION; WESTERN BLOTTING;

PROTEINS;

AGAROSE; MITOGEN ACTIVATED PROTEIN KINASE; PHOSPHOPROTEIN; PROTEIN SHC;

ARTICLE; CELL LYSATE; CENTRIFUGATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; WESTERN BLOTTING;

PHOS;

EID: 65049086456     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.03.026     Document Type: Article

References (6)

1. Kaufmann H., Bailey J.E., and Fussenegger M. Use of antibodies for detection of phosphorylated proteins separated by two-dimensional gel electrophoresis. Proteomics 1 (2001) 194-199
2. Kinoshita E., Yamada A., Takeda H., Kinoshita-Kikuta E., and Koike T. Novel immobilized zinc(II) affinity chromatography for phosphopeptides and phosphorylated proteins. J. Sep. Sci. 28 (2005) 155-162
3. Citri A., and Yarden Y. EGF-ERBB signalling: towards the systems level. Nat. Rev. Mol. Cell Biol. 7 (2006) 505-516
4. Kinoshita-Kikuta E., Kinoshita E., Yamada A., Endo M., and Koike T. Enrichment of phosphorylated proteins from cell lysate using a novel phosphate-affinity chromatography at physiological pH. Proteomics 6 (2006) 5088-5095
5. Kinoshita-Kikuta E., Aoki Y., Kinoshita E., and Koike T. Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis. Mol. Cell. Proteomics 6 (2007) 356-366
6. Kinoshita E., Kinoshita-Kikuta E., Takiyama K., and Koike T. Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Mol. Cell. Proteomics 5 (2006) 749-757