Purification and characterization of a lectin isolated from the submerged cultivated mycelium of grey polypore cerrena unicolor (Bull.) Murrill (Aphyllophoromycetideae)

International Journal of Medicinal Mushrooms

Volumn 11, Issue 1, 2009, Pages 61-68

  Mikiashvili, Nona ^a   Elisashvili, Vladimir ^b   Worku, Mulumebet ^a   Davitashvili, Elene ^b   Isikhuemhen, Omoanghe S ^a  

^a NORTH CAROLINA A AND T STATE UNIVERSITY   (United States)

^b INSTITUTE OF PHYSICS   (Georgia)

Author keywords

Cerrena unicolor; Lectin properties; Lectin puri? cation; Medicinal mushrooms; Mycelial biomass

Indexed keywords

AMMONIUM SULFATE; BARIUM; COPPER; GALACTOSE; HYDROCHLORIC ACID; IRON; LACTOSE; LECTIN; MAGNESIUM; N ACETYLGALACTOSAMINE; SODIUM PEROXIDE;

AGAR MEDIUM; APHYLLOPHOROMYCETIDEAE; ARTICLE; CERRENA UNICOLOR; DRUG ISOLATION; DRUG PURIFICATION; FUNGUS; GEL FILTRATION; HEMAGGLUTINATION; MENTAL CONCENTRATION; MUSHROOM; MYCELIUM; NONHUMAN; PRECIPITATION; SUBMERGED FERMENTATION; TEMPERATURE;

BASIDIOMYCOTA; CERRENA UNICOLOR; FUNGI;

EID: 64949123970     PISSN: 15219437     EISSN: None     Source Type: Journal    
DOI: 10.1615/IntJMedMushr.v11.i1.70     Document Type: Article

References (22)

1. Goldstein IJ, Poretz RD. Isolation, physicochemical characterization, and carbohydrate-binding specifi city of lectins. In: Liener IE, Sharon N, Goldstein IJ, editors. The lectins: properties, functions, and applications in biology and medicine. New York: Academic Press; 1986. p. 33-245.
2. Northcote DH. Lectin in the encyclopedia of molecular biology. In: Kendrew J, editor. Oxford (UK): Blackwell Science; 1994. p. 578-80.
3. Guillot J, KoŃska G. Lectins in higher fungi. Biochem Syst Ecol. 1997;25:203-30.
4. Wang HX, Ng TB. A novel lectin with potent antitumor, mitogenic and HIV-1 reverse transcriptase inhibitory activities from the edible mushroom Pleurotus citrinopileatus. Biochim Biophys Acta. 2007;1780:51-7.
5. Zatta PF, Cummings RD. Lectins and their uses as biotechnological tools. Biochem Educ. 1992;20:1-9.
6. Ng TB. Peptides and proteins from fungi. Peptides. 2004;25:1055-73.
7. Stepanova LV, Schelud'ko AV, Katsy EI, Ponomareva EG, Nikitina VE. The role of lectin-carbohydrate biospecifi c interactions between medicinal Basidiomycetes mushroom Grifola frondosa (Dicks.: Fr.) S. F. Gray and Azospirillum brasilense during their co-cultivation. Int J Med Mushr. 2008;104:65-72.
8. Musilek M, Ticha M, Volc J, Kocourek J. Studies on lectins. LXXL. Lectins in mycelial cultures of Kuehneromyces mutabilis, Pholiota squarrosa, and Flammulina velutipes. Lectins Biol Biochem Clin Biochem. 1990;7:53-6.
9. Kawagishi H, Mitsunaga S, Yamawaki M, Ido M, Shimada A, Kinoshita T, Murata T, Usui T, Kimura A, Chiba S. A lectin from the mycelia of fungus Ganoderma lucidum. Phytochemistry. 1997;44:7-10.
10. Tsivileva OM, Nikitina VE, Garibova LV. Effect of culture medium composition on the activity of extracellular lectins of Lentinus edodes. Appl Biochem Microbiol. 2006;41:174-6.
11. Ogawa S, Nakajima E, Nagao H, Ohtoshi M, Ando A, Nagata Y. Synthesis of a lectin in both mycelia and fruit bodies of the ascomycete mushroom Aleuria aurantia. Biosci Biotechnol Biochem. 1998;62:915-8.
12. She QB, Ng TB, Liu WK. A novel lectin with potent immunomodulatory activity isolated from both fruiting bodies and cultured mycelia of the edible mushroom Valvariella volvacea. Biochem Biophys Res Commun. 1998;247:106-11.
13. Mikiashvili N, Elisashvili V, Wasser SP, Nevo E. The comparative study of lectin activity of higher Basidiomycetes. Int J Med Mushr. 2006;8:30-8.
14. Wang H, Gao J, Ng TB. A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem Biophys Res Commun. 2000;275:810-6.
15. Wang HX, Liu WK, Ng TB, Ooi VEC, Chang ST. The immunomodulatory and antitumor activities of lectins from the mushroom Tricholoma mongolicum. Immuno pharmacology. 1996;31:205-11.
16. Kawagishi H, Mori A, Kimura UA, Chiba S. A sialic acid-binding lectin from the mushroom Hericium erinaceus. FEBS Lett. 1994;340:56-8.
17. Louri OH, Rosebrough NJ, Fan AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem. 1951;193:265-75.
18. Guliot J, Genaud L, Gueugnot J, Damez M. Purifi cation and properties of two hemagglutinins of the mushroom Laccaria amethystina. Biochemistry. 1983;22:5365-9.
19. Conrad F, Rudiger H. The lectin from Pleurotus ostreatus, purifi cation, characterization and interaction with a phosphatase. Phytochemistry. 1994;36:277-83.
20. Zhuang C, Murata T, Usui T, Kawagishi H, Kobayashi K. Purifi cation and characterization of a lectin from the toxic mushroom Amanita pantherina. Biochem Biophys Acta. 1996;1291:40-4.
21. Wang HX, Ng TB. Isolation of a ribonuclease from fruiting bodies of the wild mushroom Termitomyces globulus. Peptides. 2003;24:973-7.
22. Ngai PH, Ng TB. A mushroom (Ganoderma capense) lectin with spectacular thermostability, potent mitogenic activity on splenocytes, and antiproliferative activity toward tumor cells. Biochem Biophys Res Commun. 2004;314:988-93.