Isolation and characterization of endophytic actinomycetes from Chinese cabbage roots as antagonists to Plasmodiophora brassicae

Journal of Microbiology and Biotechnology

Volumn 18, Issue 11, 2008, Pages 1741-1746

  Lee, Sun Og ^a   Choi, Gyung Ja ^a   Choi, Yong Ho ^a   Jong, Kyoung Soo ^a   Park, Dong Jin ^b   Kim, Chang Jin ^b   Kim, Jin Cheol ^a  

^a Korea Research Institute of Chemical Technology   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Clubroot; Endophytic actinomycete; Microbispora rosea subsp. rosea; Plasmodiophora brassicae; Streptomyces olivochromogenes

Indexed keywords

DNA 16S; RNA 16S;

ACTINOBACTERIA; ARTICLE; BACTERIAL CELL WALL; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BIOLOGICAL CONTROL AGENT; BIOLOGICAL PEST CONTROL; CHINESE CABBAGE; CLUBROOT DISEASE; CONTROLLED STUDY; DNA SEQUENCE; ENDOPHYTE; GERMINATION; IN VIVO STUDY; INOCULATION; KOREA; MICROMONOSPORA; MORPHOLOGICAL TRAIT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PLANT GROWTH; PLANT ROOT; PLANT SEED; SEQUENCE ANALYSIS; STREPTOMYCES; STREPTOMYCES OLIVOCHROMOGENES; ACTINOMYCETALES; ANTIBIOSIS; BRASSICA; CLASSIFICATION; FUNGUS; FUNGUS SPORE; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR GENETICS; PLANT DISEASE; ULTRASTRUCTURE;

ACTINOBACTERIA; ACTINOBACTERIA (CLASS); BRASSICA RAPA SUBSP. PEKINENSIS; MICROBISPORA; MICROBISPORA ROSEA SUBSP. ROSEA; MICROMONOSPORA; PLASMODIOPHORA BRASSICAE; STREPTOMYCES; STREPTOMYCES OLIVOCHROMOGENES;

ACTINOMYCETALES; ANTIBIOSIS; BRASSICA; FUNGI; MOLECULAR SEQUENCE DATA; PEST CONTROL, BIOLOGICAL; PHYLOGENY; PLANT DISEASES; PLANT ROOTS; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SPORES, FUNGAL;

EID: 64549136287     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0800.108     Document Type: Article

References (30)

1. Bacon, C. and J. F. White. 2000. Microbial Endophytes. Marcel Dekker, New York.
2. Bressan, W. 2003. Biological control of maize seed pathogenic fungi by use of actinomycetes. Biocontrol 48: 233-240.
3. Cao, L., Z. Qiu, J. Tou, H. Tan, and S. Zhou. 2005. Isolation and characterization of endophytic streptomycete antagonists of Fusarium wilt pathogen from surface-sterilized banana roots. FEMS Microbiol. Lett. 247: 147-152.
4. Cao, L., Z. Qiu, J. You, H. Tan, and S. Zhou. 2004. Isolation and characterization of endophytic Streptomyces strains from surface-sterilized tomato (Lycopersicon esculentum) roots. Lett. Appl. Microbiol. 39: 425-430.
5. Cheah, L.-H. and B. B. C. Page. 1997. Trichoderma spp. for potential biocontrol of clubroot of vegetable brassicas. Proc. 50th N. Z. Plant Protection Conf. pp. 150-153.
6. Coombs, J. T., P. P. Michelsen, and C. M. M. Franco. 2004. Evaluation of endophytic actinobacteria as antagonists of Gaeumannomyces graminis var. tritici in wheat. Biol. Control 29: 359-366.
7. Coombs, J. T. and C. M. M. Franco. 2003. Isolation and identification of actinobacteria from surface-sterilized wheat root. Appl. Environ. Microbiol. 69: 5603-5608.
8. Crawford, D. L., J. M. Lynch, J. M. Whipp, and M. A. Ousley. 1993. Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Appl. Environ. Microbiol. 59: 3899-3905.
9. de Araujo, J. M., A. C. da Silva, and J. L. Azevedo. 2000. Isolation of endophytic actinomycetes from roots and leaves of maize (Zea mays L.). Braz. Arch. Biol. Technol. 434: 447-451.
10. Elsherif, M. and F. Grossmann. 1991. Investigations on biological control of some plant pathogenic fungi by fluoresecent pseudomonads using different methods of application. J. Plant Disease Prot. 98: 236-249.
11. Goodfellow, M. and T. Cross. 1984. Classification, pp. 7-164. In M. Goodfellow, M. Mordarski, and S. T. Williams (eds.), The Biology of the Actinomycetes. Academic Press, U. K.
12. Hayakawa, M. and H. Nonomura. 1987. Humic acid-vitamin agar, a new method for the selective isolation of soil actinomycetes. J. Ferment. Bioeng. 65: 501-509.
13. Kim, B.-J., G. J. Choi, K. Y. Cho, H. Yang, C. Shin, C.-H. Lee, and Y. Lim. 2002. Antifungal activities against Plsamodiophora brassicae causing club root. J. Microbiol. Biotechnol. 12: 1022-1025.
14. Kumar, S., K. Tamura, L. B. Jakobsen, and M. Nei. 2001. MEGA2: Molecular evolutionary genetics analysis software. Bioinformatics 17: 1244-1245.
15. Lee, C.-H., B.-J. Kim, G. J. Choi, K. Y. Cho, H. Yang, C. Shin, S. Min, and Y. Lim. 2002. Streptomyces with antifungal activity against rice blast causing fungus, Magnaporthe grisea. J. Microbiol. Biotechnol. 12: 1026-1028.
16. Lee, H. B., J.-W. Cho, D.-J. Park, C.-T. Li, Y. H. Ko, J.-H. Song, J.-S. Koh, B.-J. Kim, and C.-J. Kim. 2004. In vivo screening for biocontrol agents (BCAs) against Streptomyces scabiei causing potato common scab. Plant Pathol. J. 20: 110-114.
17. Naiki, T., K. Kageyama, and H. Ikegami. 1978. The relation of spore density of Plasmodiophora brassicae Wor. to the root hair infection and club formation in Chinese cabbage. Ann. Phytopath. Soc. Jap. 44: 432-439.
18. Narisawa, K., K. T. Ohki, and T. Hashiba. 2000. Supression of clubroot and Verticillium yellow in Chinese cabbage in the field by the root endophytic fungus, Heteroconium chaetospira. Plant Pathol. 49: 141-146.
19. Narisawa, K., M. Shimura, F. Usuki, S. Fukuhara, and T. Hashiba. 2005. Effects of pathogen density, soil moisture, and soil pH on biological control of clubroot in Chinese cabbage by Heteroconium chaetospira. Plant Dis. 89: 285-290.
20. Narisawa, K., S. Tokumasu, and T. Hashiba. 1998. Suppression of clubroot in Chinese cabbage by the root endophytic fungus, Heteroconium chaetospira. Plant Pathol. 47: 206-210.
21. Okazaki, T. 2006. Intrigued by actinomycete diversity. Actinomycetologica 20: 15-22.
22. Pospiech, A. and B. Neumann. 1995. A versatile quick-prep of genomic DNA from Gram-positive bacteria. Trends Genet. 11: 217-218.
23. Sardi, P., M. Saracchi, S. Quaroni, B. Petrolini, G. E. Borgonovi, and S. Merli. 1992. Isolation of endophytic Streptomyces strains from surface-sterilized roots. Appl. Environ. Microbiol. 58: 2691-2693.
24. Sessitsch, A., B. Reiter, U. Pfeifer, and E. Wilhelm. 2002. Cultivation-independent population analysis of bacterial endophytes in three potato varieties based on eubacterial and Actinomycetes-specific PCR of 16S rRNA genes. FEMS Microbiol. Ecol. 39: 23-32.
25. Sigler, W. V., C. H. Nakatsu, Z. J. Reicher, and R. F. Turco. 2001. Fate of the biological control agent Pseudomonas aureofaciens TX-1 after application to turfgrass. Appl. Environ. Microbiol. 67: 3542-3548.
26. Strobel, G. A. and B. Daisy. 2003. Bioprospecting for microbial endophytes and their natural products. Microbiol. Mol. Biol. Rev. 67: 491-502.
27. Taechowisan, T., J. F. Peberdy, and S. Lumyoung. 2003. Isolation of endophytic actinomycetes from selected plants and their antifungal activity. World J. Microbiol. Biotechnol. 19: 381-385.
28. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 24: 4673-4680.
29. Tian, X. L., L. X. Cao, H. M. Tan, Q. Z. Zeng, Y. Y. Jia, W. Q. Han, and S. N. Zhou. 2004. Study on the communities of endophytic fungi and endophytic actinomycetes from rice and their antipathogenic activities in vitro. World J. Microbiol. Biotechnol. 20: 303-309.
30. Yuan, W. M. and D. L. Crawford. 1995. Characterization of Streptomyces lydicus WYEC108 as a potential biological agent against fungal root and seed rots. Appl. Environ. Microbiol. 61: 3119-3128.