Thiopurine S-methyltransferase genotype-phenotype concordance: Used as a quality assurance tool to help control the phenotype assay

Annals of Clinical Biochemistry

Volumn 46, Issue 2, 2009, Pages 152-154

  Ford, Loretta ^a   Kampanis, Petros ^a   Berg, Jonathan ^a  

^a CITY HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

THIOPURINE METHYLTRANSFERASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME BLOOD LEVEL; GENOTYPE; GENOTYPE PHENOTYPE CORRELATION; HUMAN; PHENOTYPE; PRIORITY JOURNAL; QUALITY CONTROL;

GENOTYPE; HUMANS; METHYLTRANSFERASES; PHENOTYPE;

EID: 64549121174     PISSN: 00045632     EISSN: 00045632     Source Type: Journal    
DOI: 10.1258/acb.2008.008167     Document Type: Article

References (6)

1. Anstey AV, Wakelin S, Reynolds NJ. Guidelines for prescribing azathioprine in dermatology. Br J Dermatol 2004;151:1123-32
2. Gardiner SJ, Gearry RB, Begg EJ, Zhang M, Barclay ML. Thiopurine dose in intermediate and normal metabolizers of thiopurine methyltransferase may differ three-fold. Clin Gastro Haem 2008;6:654-60
3. Ford L, Graham V, Berg JD. Whole-blood thiopurine S-methyltransferase activity with genotype concordance: a new, simplified phenotyping assay. Ann Clin Biochem 2006;43:354-60
4. Schaeffeler E, Fischer C, Brockmeier D, et al. Comprehensive analysis of thiopurine S-methyltransferase phenotype-genotype correlation in a large population of German-Caucasians and identification of novel TPMT variants. Pharmacogenetics 2004;14:407-17
5. Ford LT, Berg JD. Determination of thiopurine S-methyltransferase activity in erythrocytes using 6-thioguanine as substrate and a non-extraction liquid chromatographic technique. J Chrom B 2003;798:111-5
6. Ford L, Prout C, Gaffney D, Berg J. Whose TPMT activity is it anyway? Ann Clin Biochem Clin Case 2004;41:498-500