Purification of chymotrypsin from bovine pancreas using precipitation with a strong anionic polyelectrolyte

Process Biochemistry

Volumn 44, Issue 5, 2009, Pages 588-592

  Boeris, Valeria ^a   Romanini, Diana ^a   Farruggia, Beatriz ^a   Picó, Guillemo ^a  

^a UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

Author keywords

Chymotrypsin; Polyvinyl sulfonate; Protein precipitation

Indexed keywords

ANIONIC POLYELECTROLYTES; BIOLOGICAL ACTIVITIES; BOUND MOLECULES; CHYMOTRYPSIN; INITIAL ACTIVITIES; PH CHANGES; POLYELECTROLYTE COMPLEXES; POLYELECTROLYTE MOLECULES; POLYVINYL SULFONATE; PROTEIN PRECIPITATION; SOLUBLE COMPLEXES; SPECIFIC ACTIVITIES; STOICHIOMETRIC RATIOS;

CENTRIFUGATION; DISSOLUTION; ENZYMES; POLYELECTROLYTES; PRECIPITATION (CHEMICAL); RHENIUM COMPOUNDS;

POLYMERS;

BOVINAE;

EID: 64449084720     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2009.02.009     Document Type: Article

References (10)

1. Matsudo T., Ogawa K., and Kokufuta E. Complex formation of protein with different water-soluble synthetic polymers. Biomacromolecules 4 (2003) 1794-1799
2. Chatterjee S., Chatterjee S., Chatterjee B.P., and Guha A.K. Clarification of fruit juice with chitosan. Process Biochem 39 (2004) 2229-2232
3. Sharmat S.K., and Hopkins T.R. Activation of bovine chymotrypsinogen A. Isolation and characterization of chymotrypsin. Biochemistry 18 (1979) 1008-1013
4. Boeris V., Spelzini D., Peleteiro Salgado J., Picó G., Romanini D., and Farruggia B. Chymotrypsin-poly vinyl sulfonate interaction studied by dynamic light scattering and turbidimetric approaches. Biochim Biophys A 1780 (2008) 1032-1037
5. Guyonneta V., Çuscikb F.T., Longa P.L., Polanowskib A., and Travis J. Purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin and elastase from the chicken. J Chromatogr A 852 (1999) 217-225
6. Patrickios C.S., Sharma L.R., Armes S.P., and Billingham N.C. Precipitation of a water-soluble ABC triblock methacrylic polyampholyte: effects of time, pH, polymer concentration, salt type and concentration, and presence of a protein. Langmuir 15 (1999) 1613-1620
7. Romanini D., Braia M., Giatte Angarten R., Loh W., and Picó G. Interaction of lysozyme with negatively charged flexible chain polymers. J Chromatogr B 857 (2007) 25-31
8. Boeris V., Farruggia B., Nerli B., Romanini D., and Picó G. Protein-flexible chain polymer interactions to explain protein partition in aqueous two-phase systems and the protein-polyelectrolyte complex formation. I. J Biol Macromol 41 (2007) 286-294
9. Chatterjee S., Adhya M., Guha A.K., and Chatterjee B.P. Chitosan from Mucor rouxii: production and physico-chemical characterization. Process Biochem 40 (2005) 395-400
10. Izumrudov V.A., Galaev I.Y., and Mattiasson B. Polycomplexes-potential for bioseparation. Bioseparation 7 (1999) 207-220