Preferential hydrolysis of aberrant intermediates by the type II thioesterase in Escherichia coli nonribosomal enterobactin synthesis: Substrate specificities and mutagenic studies on the active-site residues

Biochemistry

Volumn 48, Issue 8, 2009, Pages 1712-1722

  Guo, Zu Feng ^a   Sun, Yueru ^a   Zheng, Suilan ^a   Guo, Zhihong ^a  

^a HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDIC RESIDUES; ACTIVE SITES; ARTHROBACTER SP; ASSEMBLY LINES; CATALYTIC BASE; CATALYTIC EFFICIENCIES; DIFFERENTIAL INTERACTIONS; DIHYDROXYBENZOATE; EFFICIENT RESTORATIONS; ENTEROBACTIN; HYDROXYL GROUPS; IN-VITRO; KINETIC STUDIES; NONRIBOSOMAL PEPTIDES; SEARCH MECHANISMS; SITE-DIRECTED MUTAGENESIS; STRUCTURAL COMPARISONS; SUBSTRATE HYDROXYLATIONS; SUBSTRATE SPECIFICITIES; THIOESTER; THIOESTERASE; THIOESTERASES; THIOESTERS; TYPE II;

AMINES; AMINO ACIDS; ASSEMBLY; ASSEMBLY MACHINES; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; BIOSYNTHESIS; ESCHERICHIA COLI; HYDROXYLATION;

SUBSTRATES;

2,3 DIHYDROXYBENZOIC ACID; ALANINE; ENTEROCHELIN; GLUTAMIC ACID; HISTIDINE; HYDROXYL GROUP; METHIONINE; NUCLEOPHILE; PANTETHEINE; PANTETHEINE PHOSPHATE; SALICYLIC ACID; SERINE; THIOESTER; THIOL ESTER HYDROLASE; THREONINE;

ARTHROBACTER; ARTICLE; BACTERIAL STRAIN; BIOSYNTHESIS; CATALYSIS; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; HYDROLYSIS; HYDROXYLATION; MUTAGENESIS; NONHUMAN; NONRIBOSOMAL PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN INTERACTION; PROTEIN SYNTHESIS; SITE DIRECTED MUTAGENESIS; STEADY STATE;

AMINO ACIDS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ENTEROBACTIN; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FATTY ACID SYNTHETASE COMPLEX; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; HYDROXYBENZOIC ACIDS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; RIBOSOMES; SUBSTRATE SPECIFICITY; TEMPERATURE; THIOLESTER HYDROLASES;

ARTHROBACTER SP.; ESCHERICHIA COLI;

EID: 64349120214     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi802165x     Document Type: Article

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