Application of polymerase chain reaction-restriction fragment length polymorphism analysis and lab-on-a-chip capillary electrophoresis for the specific identification of game and domestic meats

Journal of the Science of Food and Agriculture

Volumn 89, Issue 5, 2009, Pages 843-847

  Fajardo, Violeta ^a   González, Isabel ^a   Dooley, John ^b   Garret, Steve ^b   Brown, Helen M ^b   García, Teresa ^a   Martín, Rosario ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b CAMPDEN BRI   (United Kingdom)

Author keywords

12S rRNA gene; Capillary electrophoresis; Game meat; PCR RFLP

Indexed keywords

EID: 63849338866     PISSN: 00225142     EISSN: 10970010     Source Type: Journal    
DOI: 10.1002/jsfa.3522     Document Type: Article

References (16)

1. Brodmann PD, Nicholas G, Schaltenbrand P and Ilg EC, Identifying unknown game species: experience with nucleotide sequencing of the mitochondrial cytochrome b gene and a subsequent basic local alignment search tool search. Eur Food Res Technol 212:491-496 (2001).
2. Wolf C, Rentsch J and Hübner P, PCR-RFLP analysis of mitochondrial DNA: a reliable method for species identification. J Agric Food Chem 47:1350-1355 (1999).
3. Pfeiffer I, Burger J and Brenig B, Diagnostic polymorphisms in the mitochondrial cytochrome b gene allow discrimination between cattle, sheep, goat, roe buck and red deer by PCR-RFLP. Genetics 5:30-35 (2004).
4. Woolfe M and Primrose S, Food forensics: using DNA technology to combat misdescription and fraud. Trends Biotechnol 22:222-226 (2004).
5. Partis L, Croan D, Guo Z, Clark R, Coldham T and Murby J, Evaluation of a DNA fingerprinting method for determining the species origin of meats. Meat Sci 54:369-376 (2000).
6. Saini M, Das DK, Dhara A, Swarup D, Yadav MP and Gupta PK, Characterisation of peacock (Pavocristatus) mitochondrial 12S rRNA sequence and its use in differentiation from closely related poultry species. Br Poult Sci 48:162-166 (2007).
7. Abdel-Rahman SM and Ahmed MMM, Rapid and sensitive identification of buffalo's, cattle's and sheep's milk using species-specific PCR and PCR-RFLP techniques. Food Control 18:1246-1249 (2007).
8. Dolnik V, Liu S and Jovanvich S. Capillary electrophoresis on microchip. Electrophoresis 21:41-54 (2000).
9. Panaro NJ, Yuen PK, Sakazume T, Fortina P, Kricka LJ and Wilding P, Evaluation of DNA fragment sizing and quantification by the Agilent 2100 Bioanalyzer. Clin Chem 46:1851-1853 (2000).
10. Nachamkin I, Panaro NJ, Li M, Ung H, Yuen PK, Kricka LJ, et al, Agilent 2100 bioanalyzer for restriction fragment length polymorphism analysis of the Campylobacter jejuni flagellin gene. J Clin Microbiol 39:754-757 (2001).
11. Fajardo V, González I, López-Calleja I, Martín I, Hernández PE, García T, et al, PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries) and goat (Capra hircus). JAgric Food Chem 54:1144-1150 (2006).
12. Fajardo V, González I, López-Calleja I, Martín I, Rojas M, Hernández PE, et al, Analysis of mitochondrial DNA for authentication of meats from chamois (Rupicapra rupicapra), pyrenean ibex (Capra pyrenaica), and mouflon (Ovis ammon) by polymerase chain reaction-restriction fragment length polymorphism. JAOACInt 90:179-196 (2007).
13. Teletchea F, Maudet C and Hänni C, Food and forensic molecular identification: update and challenges. Trends Biotechnol 23:359-366 (2005).
14. Sun YL and Lin CS, Establishment and application of a fluorescent polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying porcine, caprine, and bovine meats. J Agric Food Chem 51:1771-1776 (2003).
15. Dooley JJ, Sage HD, Clarke ML, Brown HH and Garrett SD, Fish species identification using PCR-RFLP analysis and lab-on-a-chip capillary electrophoresis: application to detect white fish species in food products and an interlaboratory study. J Agric Food Chem 53:3348-3357 (2005).
16. Dooley JJ, Garrett SD and Brown HH, Improved fish species identification by use of lab-on-a-chip technology. Food Control 16:601-607 (2005).