A novel assay of bacterial peptidoglycan synthesis for natural product screening

Journal of Antibiotics

Volumn 62, Issue 3, 2009, Pages 153-158

  Murakami, Ryo ^a   Muramatsu, Yasunori ^a   Minami, Emiko ^a   Masuda, Kayoko ^a   Sakaida, Yoshiharu ^a   Endo, Shuichi ^a   Suzuki, Takashi ^a   Ishida, Osamu ^a   Takatsu, Toshio ^a   Miyakoshi, Shunichi ^a,b   Inukai, Masatoshi ^a,c   Isono, Fujio ^a  

^a DAIICHI SANKYO CO   (Japan)

^b GUNMA NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

^c INTERNATIONAL UNIVERSITY OF HEALTH AND WELFARE   (Japan)

Author keywords

Cycloserine; D Ala D Ala biosynthesis; MurF; Peptidoglycan; Screening; Translocase I

Indexed keywords

A 94964; ALANINE RACEMASE; ALANYLALANINE LIGASE; ALANYLALANINE TRANSFERASE; BACTERIAL ENZYME; CAPRAZAMYCIN DERIVATIVE; CAPURAMYCIN DERIVATIVE; CYCLOSERINE; DANSYL CHLORIDE; LIPOSIDOMYCIN DERIVATIVE; MURAYMYCIN DERIVATIVE; MUREIDOMYCIN DERIVATIVE; NAPSAMYCIN DERIVATIVE; NATURAL PRODUCT; PACIDAMYCIN DERIVATIVE; PEPTIDOGLYCAN; TRANSLOCASE I; TUNICAMYCIN; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE N ACETYLMURAMIC ACID;

ANTIBIOTIC BIOSYNTHESIS; ARTICLE; BACILLUS CEREUS; DRUG STRUCTURE; ENZYME ACTIVITY; FERMENTATION; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; IC 50; MICROBIAL METABOLISM; NONHUMAN; PLASMID; PRIORITY JOURNAL;

BACTERIA; BIOLOGICAL PRODUCTS; CARBOHYDRATE SEQUENCE; CELL-FREE SYSTEM; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYCLOSERINE; ESCHERICHIA COLI; FERMENTATION; FLUORESCENCE; MOLECULAR CONFORMATION; MOLECULAR SEQUENCE DATA; PEPTIDE SYNTHASES; PEPTIDOGLYCAN; PLASMIDS; SPECTROMETRY, FLUORESCENCE; URIDINE DIPHOSPHATE N-ACETYLMURAMIC ACID;

BACTERIA (MICROORGANISMS);

EID: 63849180533     PISSN: 00218820     EISSN: None     Source Type: Journal    
DOI: 10.1038/ja.2009.4     Document Type: Article

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