Using microbial fatty acids to improve understanding of the contribution of solid associated bacteria to microbial mass in the rumen

Animal Feed Science and Technology

Volumn 150, Issue 3-4, 2009, Pages 197-206

  Bessa, R J B ^a   Maia, M R G ^a   Jeronimo E ^a   Belo, A T ^b   Cabrita, A R J ^c   Dewhurst, R J ^d   Fonseca, A J M ^e  

^a Instituto Nacional de Recursos Biologicos   (Portugal)

^b Instituto Nacional de Recursos Biologicos   (Portugal)

^c Faculdade de Farmácia da Universidade do Porto   (Portugal)

^d TEAGASC   (Ireland)

^e UNIVERSITY OF PORTO   (Portugal)

Author keywords

Microbial markers; Odd and branched chain fatty acids; Purine bases; Rumen bacteria

Indexed keywords

BACTERIA (MICROORGANISMS); MEDICAGO SATIVA;

EID: 63649109928     PISSN: 03778401     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.anifeedsci.2008.09.005     Document Type: Article

References (33)

1. AOAC. Official Methods of Analysis. 15th ed. (1990), Association of Official Analytical Chemists, Arlington, VA, USA
2. Balcells J., Ganuza J.M., Pérez J.F., Martín Orúe S.M., and Ronquillo M.G. Urinary excretion of purine derivatives as an index of microbial-nitrogen intake in growing rabbits. Br. J. Nutr. 79 (1998) 373-380
3. Bates D.B., Gillet J.A., Barao S.A., and Bergen W.G. The effect of specific growth rate and stage of growth on nucleic acid-protein values of pure cultures and mixed ruminal bacteria. J. Anim. Sci. 61 (1985) 713-724
4. Bauchart D., Legay-Carmier F., Doreau M., and Gaillard B. Lipid metabolism in liquid-associated and solid-adherent bacteria in rumen contents of dairy cows offered lipid supplements diets. Br. J. Nutr. 63 (1990) 563-578
5. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
6. Broderick G.A., and Merchen N.R. Markers for quantifying microbial protein synthesis in the rumen. J. Dairy Sci. 75 (1992) 2618-2632
7. Craig W.M., Broderick G.A., and Ricker D.B. Quantitation of microorganisms associated with the particulate phase of ruminal ingesta. J. Nutr. 117 (1987) 56-62
8. Diedrich M., and Henschel K.P. The natural occurrence of unusual fatty-acids. 1. Odd numbered fatty-acids. Nahrung 34 (1990) 935-943
9. Djouvinov D.S., Nakashima Y., Todorov N., and Pavlov D. In situ degradation of feed purines. Anim. Feed Sci. Technol. 71 (1998) 67-77
10. Gonzaléz-Ronquilho M., Balcells J., Belenguer J., Castrillo C., and Mota M. A comparison of purine derivatives excretion with conventional methods as indices of microbial yield in dairy cows. J. Dairy Sci. 87 (2004) 2211-2221
11. Harfoot C.G., Noble R.C., and Moore J.H. The role of plant particles, bacteria and cell-free supernatant fractions of rumen contents in the hydrolysis of trilinolein and the subsequent hydrogenation of linoleic acid. Antonie van Leeuwenhoek 41 (1975) 533-542
12. Kaneda T. Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance. Microbiol. Rev. 55 (1991) 288-302
13. Kim E.J., Sanderson R., Dhanoa M.S., and Dewhurst R.J. Fatty acid profiles associated with microbial colonization of freshly ingested grass and rumen biohydrogenation. J. Dairy Sci. 88 (2005) 3230
14. Legay-Carmier F., and Bauchart D. Distribution of bacteria in the rumen contents of dairy cows given a diet supplemented with soya-bean oil. Br. J. Nutr. 61 (1989) 725-740
15. Makkar H.P.S., and Becker K. Purine quantification in digesta from ruminants by spectrophotometric and HPLC methods. Br. J. Nutr. 81 (1999) 107-112
16. Martin C., Williams A.G., and Michalet-Doreau B. Isolation and characteristics of the protozoal and bacterial fractions from bovine ruminal contents. J. Anim. Sci. 72 (1994) 2962-2968
17. Martín-Orúe S.M., Balcells J., Zakraoui F., and Castrillo C. Quantification and chemical composition of mixed bacteria harvested from solid fractions of rumen digesta, effect of detachment procedure. Anim. Feed Sci. Technol. 71 (1998) 269-282
18. Merry R.J., and McAllan A.B. A comparison of the chemical composition of mixed bacteria harvested from the liquid and solid fractions of rumen digesta. Br. J. Nutr. 50 (1983) 701-709
19. Minato H., Endo A., Higuchi M., Ootomo Y., and Uemura T. Ecological treatise on the rumen fermentation I. The fractionation of bacteria attached to the rumen digesta solids. J. Gen. Microbiol. 12 (1966) 39-52
20. Moss C.W. Gas-liquid chromatography as an analytical tool in microbiology. J. Chromatogr. 203 (1981) 337-347
21. Olsson P.A. Signature fatty acids provide tools for determination of the distribution and interactions of mycorrhizal fungi in soil. FEMS Microbiol. Ecol. 29 (1999) 303-310
22. Olubobokun J.A., and Craig W.M. Quantity and characteristics of microorganisms associated with ruminal fluid or particles. J. Anim. Sci. 68 (1990) 3360-3370
23. Perez J.F., Balcells J., Fondevila M., and Guada J.A. Composition of liquid- and particle-associated bacteria and their contribution to the rumen outflow. Aust. J. Agric. Res. 49 (1998) 907-914
24. Rodriguez C.A., Gonzalez J., Alvir M.R., Repetto J.L., Centeno C., and Lamrani F. Composition of bacteria harvested from the liquid and solid fractions of the rumen of sheep as influenced by feed intake. Br. J. Nutr. 84 (2000) 369-376
25. SAS, 2001. User's Guide. Statistics, Release 8.2. SAS Inst. Inc., Cary, NC, USA.
26. Sukhija P.S., and Palmquist D.L. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. J. Agric. Food Chem. 36 (1988) 1202-1206
27. Van Soest P.J., Robertson J.B., and Lewis B.A. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74 (1991) 3583-3597
28. Vicente F., Guada J.A., Surra J., Balcells J., and Castrillo C. Microbial contribution to duodenal purine flow in fattening cattle given concentrate diets, estimated by purine N labelling (N-15) of different microbial fractions. Anim. Sci. 78 (2004) 159-167
29. Vlaeminck B., Dufour C., Van Vuuren A.M., Cabrita A.R.J., Dewhurst R.J., Demeyer D., and Fievez V. Use of odd and branched-chain fatty acids in rumen contents in rumen contents and milk as a potential microbial marker. J. Dairy Sci. 88 (2005) 1031-1042
30. Vlaeminck B., Fievez V., Cabrita A.R.J., Fonseca A.J.M., and Dewhurst R.J. Factors affecting odd- and branched-chain fatty acids in milk: a review. Anim. Feed Sci. Technol. 131 (2006) 389-417
31. Vlaeminck B., Fievez V., Demeyer D., and Dewhurst R.J. Effect of forage:concentrate ratio on fatty acid composition of rumen bacteria isolated from ruminal and duodenal digesta. J. Dairy Sci. 89 (2006) 2668-2678
32. Volden H., Mydland L.T., and Harstad O.M. Chemical composition of protozoal and bacterial fractions isolated from ruminal contents of dairy cows fed diets differing in nitrogen supplementation. Acta Agric. Scand. A 49 (1999) 235-244
33. Yang W.Z., Beauchemin K.A., and Rode L.M. Effect of dietary factors on distribution and chemical composition of liquid- or solid-associated bacterial populations in the rumen of dairy cows. J. Anim. Sci. 79 (2001) 2736-2746