Plasmid-encoded protein: The principal factor in the "metabolic burden" associated with recombinant bacteria

Biotechnology and Bioengineering

Volumn 102, Issue 5, 2009, Pages 1284-1297

  Bentle, William E ^a,b,c   Mirjalili, Noushin ^a   Andersen, Dana C ^a   Davis, Robert H ^a   Kompala, Dhinakar S ^a  

^a University of Colorado   (United States)

^b UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^c UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COPY NUMBERS; E. COLI; ENCODED PROTEINS; KINETIC MODELS; LACTAMASE; LINEAR DECREASE; LINEAR RELATIONSHIPS; LOW GROWTH RATES; MEDIA INDEPENDENTS; MODEL PREDICTIONS; OPTIMAL CONDITIONS; PRINCIPAL FACTORS; PRODUCT YIELDS; PROTEIN CONTENTS; PROTEIN EXPRESSIONS; RECOMBINANT BACTERIA;

BACTERIOLOGY; BIOCHEMISTRY; ESCHERICHIA COLI; KINETIC THEORY; METABOLISM;

GROWTH RATE;

BETA LACTAMASE; CHLORAMPHENICOL; CHLORAMPHENICOL ACETYLTRANSFERASE;

ARTICLE; BACTERIAL METABOLISM; CELL GROWTH; CELL METABOLISM; CONTROLLED STUDY; ESCHERICHIA COLI; GROWTH RATE; NONHUMAN; PLASMID; PROTEIN CONTENT; PROTEIN EXPRESSION; SPECTROPHOTOMETRY;

BACTERIA; BIOTECHNOLOGY; PLASMIDS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 63549099263     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. T. lmanaka and S. Aiba, Ann. NY Acad. Sci., 369, 1 (1981).
2. F. Fuller. Gene. 19, 43 (1982).
3. E. Remaut. P. Stanssens, and W. Fiers, Gene, 15, 81 (1981).
4. E. Arnann. J. Brosius, and M. Ptashne, Gene, 25, 167 (1983).
5. E. Remaut. H. Tsao, and W. Fiers, Gene, 22, 103 (1983).
6. E. L. Larsen. K. Gerdes, J. Light, and S. Molin, Gene, 28, 45 (1984).
7. A. SeressiOtis and J. E. Bailey, Biotechnol. Bioeng., 29, 392 (1987).
8. D. Lauffenburger, Ann. NY Acad. Sci., 469, 97 (1986).
9. G Stephanopoulos and G. R. Lapidus, Chem. Eng. Sci.. 43, 49 (1988).
10. D. DiBrasio and C. Sardonini. Ann. NY Acad. Sci., 469, 111 (1986).
11. J.E. Bailey. N.A. DaSilva, W. Peretti, J.-H. Seo, and F. Srienc, Ann. NY Acad. Sci., 469, 97 (1986).
12. Seo and J E. Bailey, Biotechnol. Bioeng., 27, 1668 (1985).
13. R. Siegel and D. Y. Ryu, Biotechnol. Bioeng., 27, 28 (1985).
14. M. M. Ataai and M. L. Shuler. Biotechnol. Bioeng., 30, 389 (1987).
15. WE. Bentley and D.S Kompala, Biotechnol. Bioeng., 33, 49 (1987).
16. W. E. Bentley and D.S. Kompala, "Using structured kinetic model for analyzing instability in recombinant bacterial cultures." in Frontiers in Bioprocess¡ng, S. Sikdar, P. Todd, and M. Bier. Eds. (CRC. Boca Raton. FL. 1988).
17. R.C. Tail and H. W. Boyer, Cell. 13. 73 (1978).
18. T. Maniatis, E. F. Fritsch, and J. Sambrook, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory. Cold Spring Harbor. NY, 1982).
19. R. L. Rodriguez and R. C. Tait, Recombinant DNA Techniques: An Introduction (Benjamin/Cummings, Menlo Park, CA. 1983).
20. J.R. Lupski, A. A. Ruiz, and G.N. Godson, Mol Gen. Genet., 195, 391 (1984).
21. D. S. Holmes and M. Quigley. Anal. Biochem., 114, 193 (1981).
22. W. E. Bentley and D. S. Kompala, "Optimal induction of protein expression in recombinant bacterial cultures." (unpublished).
23. J. Davison. Gene, 28, 1 (1984).
24. B. Lewin, Genes III, (Wiley, New York, 1987).
25. S. Lin-Chao and H. Bremer, Mol. Gen. Genet., 203, 143 (1986).
26. D. Stueber and H. Bujard, EMBOJ., 1(11), 1399(1982).
27. R.-A. Klotsky and I. Schwartz, Gene, 55, 141 (1987)
28. J. Koizumi. Y. Monden, and S. Aiba, Biotechnol. Bioeng., 27, 21 (1985).
29. W. E. Bentley and D. S. Kompala. "Recombinant bacterial population dynamics in continuous cultures at different dilution rates: experimental data vs. model predictions," (unpublished).
30. H. Jacobsen, Ph.D. thesis, Copenhagen University. Copenhagen, Denmark, 1974.