Genetic organization of the dhlA gene encoding 1,2-dichloroethane dechlorinase from Xanthobacter flavus UE15

Journal of Microbiology

Volumn 42, Issue 3, 2004, Pages 188-193

  Song, Ji Sook ^a   Lee, Dong Hun ^a   Lee, Kyoung ^b   Kim, Chi Kyung ^a  

^a Chungbuk National University   (South Korea)

^b Changwon National University   (South Korea)

Author keywords

1,2 dichloroethane; Dehalogenase; dhlA; Xanthobacter flavus UE15

Indexed keywords

1,2 DICHLOROETHANE DECHLORINASE; CHLORINE DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DECHLORINATION; GENE INSERTION; GENE LOCATION; GENETIC ANALYSIS; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; SEQUENCE HOMOLOGY; WASTE WATER; XANTHOBACTER; XANTHOBACTER FLAVUS;

BACTERIAL PROTEINS; CLONING, MOLECULAR; DNA, RIBOSOMAL; ETHYLENE DICHLORIDES; HYDROLASES; MOLECULAR SEQUENCE DATA; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; XANTHOBACTER;

INSERTION SEQUENCES; MYCOBACTERIUM; MYCOBACTERIUM SP.; PSEUDOMONAS; PSEUDOMONAS PAVONACEAE; RHODOCOCCUS; RHODOCOCCUS RHODOCHROUS; XANTHOBACTER; XANTHOBACTER AUTOTROPHICUS; XANTHOBACTER FLAVUS;

EID: 6344238764     PISSN: 12258873     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Bergman, J.G. and J. Sanik. 1957. Determination of trace amounts of chloride in naphtha. Anal. Chem. 29, 241-243.
2. Chae, J.C., Y.C. Kim, G.J. Zylstra, and C.K. Kim. 2000. Genetic structure and functional implication of the fcb gene cluster for hydrolytic dechlorination of 4-chlorobenzoate from Pseudomonas sp. DJ-12. Gene 258, 109-116.
3. Fetzner, S. and F. Lingens. 1994. Bacterial dehalogenase: biochemistry, genetics, and biotechnological applications. Microbiol. Rev. 58, 641-685.
4. Hill. K.E., J.R. Marchesi, and A.J. Weightman. 1999. Investigation of two evolutionarily unrelated halocarboxylic acid dehalogenase gene family. J. Bacteriol. 181, 2535-2547.
5. Janssen, D.B., J.E. Oppentocht, and G.J. Poelarends. 2001. Microbial dehalogenatipn. Curr. Opin. Biotechnol. 12, 254-258.
6. Janssen, D.B., F. Pries, J. Van Der Ploeg, B. Kazemier, P. Terpstra, and B. Witholt. 1989. Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene. J. Bacteriol. 171, 6791-6799.
7. Janssen, D.B., A. Scheper, L. Dijkhuizen, and B. Witholt. 1985. Degradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10. Appl. Environ. Microbiol. 49, 673-677.
8. Koetsier, P.A., J. Schorr, and W. Doerfler. 1993. A rapid optimized protocol for downward alkaline Southern blotting of DNA. Biotechniques 15, 260-262.
9. Kulakov, L.A., G.J. Poelarends, D.B. Janssen, and M.J. Larkin. 1999. Characterization of IS2112, a new insertion sequence from Rhodococcus, and its relationship with mobile elements belonging to the IS110 family. Microbiology 145, 561-568.
10. Kulakova, A.N., M.J. Larkin, and L.A. Kulakov. 1997. The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064. Microbiology 143, 109-115.
11. Magnuson, J.K., M.F. Romine, D.R. Burris, and M.T. Kingsley. 2000. Trichloroethene reductive dehalogenase from Dehalococcoides ethenogenes: sequence of tceA and substrate range characterization. Appl Environ. Microbiol. 66, 5141-5147.
12. Maymo-Gatell, X., T. Anguish, and S.H. Zinder. 1999. Reductive dechlorination of chlorinated ethenes and 1,2-dichloroethane by Dehalococcoides ethenogens 195. Appl. Environ. Microbiol. 67, 3108-3113.
13. Neumann, A., G. Wohlfarth, and G. Diekert. 1998. Tetrachloroethene dehalogenase from Dehalospirillum multivorans: cloning, sequencing of the encoding genes, and expression of the pceA gene in Escherichia coli. J. Bacteriol. 180, 4140-4145.
14. Newman, J., T.S. Peat, R. Richard, L. Kan, P.E. Swanson, J.A. Affholter, I.H. Holmes, J.F. Schindler, C.J. Unkefer, and T.C. Terwilliger. 1999. Haloalkane dehalogenases: structure of a Rhodococcus enzyme. Biochemistry 38, 16105-16114.
15. Poelarends, G.J., L.A. Kulakov, M.J. Larkin, J.E. van Hylckama Vlieg, and D.B. Janssen. 2000. Roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene and 1,2-dibromoethane-degradative pathways. J. Bacteriol. 182, 2191-2199.
16. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning, 2nd ed., Cold Spring Harbor, N.Y.
17. Sanger, F., S. Nicklen, and A.R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
18. Song, J.S. 2004. Isolation of 1,2-dichloroethane-degrading Xanthobacter flavus UE2 and UE15, and nucleotide sequences analysis of their dhlA genes responsible for hydrolytic dechlorination. M. S. thesis, Chungbuk National University.
19. Song, J.S., D.H. Lee, K. Lee, and C.K. Kim. 2003. Characteristics of several bacterial isolates capable of degrading chloroaliphatic compounds via hydrolytic dechlorination. J. Microbiol. 41, 277-283.
20. Van de Pas, B.A., H. Smidt, W.R. Hagen, J. van der Oost, G. Schraa, A.J. Stams, and W. M. de Vos. 1999. Purification and molecular characterization of ortho-chlorophenol reductive dehalogenase, a key enzyme of halorespiration in Desulfitobacterium dehalogenans. J. Biol. Chem. 274, 20287-20292.
21. Van der Ploeg. J., G. van Hall, and D.B. Janssen. 1991. Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene. J. Bacteriol. 173, 7925-7933.
22. Van der Ploeg, J., M. Willemsen, G. van Hall, and D.B. Janssen. 1995. Adaptation of Xanthobacter autotrophicus GJ10 to bromoacetate due to activation and mobilization of the haloacetate dehalogenase gene by insertion element IS1247. J. Bacteriol. 177, 1348-1356.