Dual fluorescence labeling of surface-exposed and internal proteins in erythrocytes infected with the malaria parasite plasmodium falciparum

Nature Protocols

Volumn 3, Issue 12, 2008, Pages 1990-1996

  Bengtsson, Dominique C ^a   Sowa, Kordai M P ^a   Arnot, David E ^a,b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; FLUORESCENT DYE; MEMBRANE PROTEIN;

ANIMAL; ARTICLE; CHEMISTRY; ERYTHROCYTE; FLUORESCENCE; METABOLISM; METHODOLOGY; PARASITOLOGY; PHYSIOLOGY; PLASMODIUM FALCIPARUM; STAINING;

ANIMALS; ANTIBODIES; ERYTHROCYTES; FLUORESCENCE; FLUORESCENT DYES; MEMBRANE PROTEINS; PLASMODIUM FALCIPARUM; STAINING AND LABELING;

EID: 63349103521     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.196     Document Type: Article

References (19)

1. Miyashita, T.: Confocal microscopy for intracellular co-localization of proteins. Method Mol. Biol. 261, 399-410 (2004).
2. Hall, R.: Antigens of the erythrocytes stages of the human malaria parasite Plasmodium falciparum detected by monoclonal antibodies. Mol. Biochem. Parasitol. 7, 247-265 (1983).
3. Knuepfer, E.: Trafficking of the major virulence factor to the surface of transfected P falciparum-infected erythrocytes. Blood 105, 4078-4087 (2005).
4. Maier, A.G.: Skeleton-binding protein 1 functions at the parasitophorous vacuole membrane to traffic PfEMP-1 to the Plasmodium falciparum-infected erythrocyte surface. Blood 109, 1289-1297 (2007).
5. Dahlback, M.: Changes in var gene mRNA levels during erythrocyte development in two phenotypically distinct Plasmodium falciparum parasites. Malar. J. 6,78 (2007).
6. Kreik, N.: Characterization of the pathway for transport of the cytoadherence-mediating protein, PfEMPl, to the host cell surface in malaria parasite-infected erythrocytes. Mol. Microbiol. 50, 1215-1227 (2003).
7. Zhou, Y.-H.: Positive reactions on western blots do not necessarily indicate the epitopes on antigens are continuous. Immun. Cell Biol. 85, 73-78 (2007).
8. Barfod, L.: Human pregnancy-associated malaria-specific B cells target polymorphic, conformational epitopes in VAR2CSA. Mol. Microbiol. 63, 335-347 (2007).
9. Brelje, T.C., Wessendorf, M.W. and Sorenson, R.L.: Multicolor laser scanning confocal immunofluorescence microscopy: practical application and limitations. Method Cell Biol. 70, 165-244 (2002).
10. Bacallao, R. and Stelzer, E.H.K.: Preservation ofbiologicalspecimensforobservation in a confocal fluorescence microscope. Method Cell Biol. 31, 437-452 (1989).
11. Bengtsson, D.C.: A method for visualizing surface-exposed and internal PfEMP1 adhesion antigens in Plasmodium falciparum infected erythrocytes. Malar. J. 7, 101 (2008).
12. Egorina, E.M.: Intracellular and surface distribution of monocyte tissue factor—application to inter-subject variability. Arteriosder Thromb Vasc. Biol. 25, 1493-1498 (2005).
13. Jensen, A.T.R.: Plasmodium falciparum associated with severe childhood malaria preferentially expresses PfEMP1 encoded by Group A var genes. J. Exp. Med. 199, 1179-1190 (2004).
14. Madhunapantula, S.V.: Developmental stage- and cell cycle number-dependent changes in characteristics of Plasmodium falciparum-infected erythrocyte adherence to placental chondroitin-4-sulfate proteoglycan. Infect. Immun. 75, 4409-4415 (2007).
15. Hoetelmans, R.W.M.: Effects of acetone, methanol or paraformaldehyde on cellular structure, visualized by reflection contrast microscopy and transmission and scanning electron microscopy. App. Immunohistochem. Mol. Morph. 9, 346-351 (2001).
16. Weibull, C., Christiansson, A. and Carlemalm, E.: Extraction of membrane lipids during fixation, dehydration and embedding of Acholeplasma laidlawi cells for electron microscopy. J. Microscop. 129, 201-207 (1983).
17. Melan, M.A. and Sluder, G.: Redistribution and differential extraction of soluble proteins in permeabilized cultured cells. Implications for immunofluoresence microscopy. J. Cell Sci. 101, 731-743 (1992).
18. Lucci, N.W., Peterson, D.S. and Moore, J.M.: Immunological activation of human syncytiotrophoblast by Plasmodium falciparum. Malar. J. 7, 42 (2008).
19. Creasey, A.M.: Non-specific IgM and chondroitin sulphate A binding are linked phenotypes of Plasmodium falciparum isolatesimplicated in malaria during pregnancy. Infect. Immun. 71, 4767-4771 (2003).