Biochemical, biophysical, and mutational analyses of subunit interactions of the human cytomegalovirus nuclear egress complex

Journal of Virology

Volumn 83, Issue 7, 2009, Pages 2996-3006

  Sam, My D ^a   Evans, Brady T ^a   Coen, Donald M ^a   Hogle, James M ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; HETERODIMER; HOMODIMER; MONOMER; MUTANT PROTEIN; UL50 PROTEIN; UL53 PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BIOPHYSICS; CELL MIGRATION; CELL NUCLEUS MEMBRANE; CHEMICAL ANALYSIS; DIMERIZATION; GEL PERMEATION CHROMATOGRAPHY; GENETIC TRANSFECTION; HUMAN CYTOMEGALOVIRUS; ISOTHERMAL TITRATION CALORIMETRY; MUTATIONAL ANALYSIS; NUCLEAR EGRESS COMPLEX; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN QUATERNARY STRUCTURE; ULTRACENTRIFUGATION; ACTIVE TRANSPORT; CALORIMETRY; CYTOMEGALOVIRUS; HUMAN; ISOLATION AND PURIFICATION; MACROMOLECULE; METABOLISM; PHYSIOLOGY; PROTEIN ANALYSIS; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS; VIRUS REPLICATION;

HERPESVIRIDAE; HUMAN HERPESVIRUS 5;

ACTIVE TRANSPORT, CELL NUCLEUS; AMINO ACID SUBSTITUTION; CALORIMETRY; CYTOMEGALOVIRUS; DIMERIZATION; HUMANS; MACROMOLECULAR SUBSTANCES; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; VIRAL PROTEINS; VIRUS REPLICATION;

EID: 63149109165     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.02441-08     Document Type: Article

References (25)

1. Bubeck, A., M. Wagner, Z. Ruzsics, M. Lotzerich, M. Iglesias, I. R. Singh, and U. H. Koszinowski. 2004. Comprehensive mutational analysis of a her pesviras gene in the viral genome context reveals a region essential for vims replication. J. Virol. 78:8026-8035.
2. Camozzi, D., S. Pignatelli, C. Valvo, G. Lattanzi, C. Capanni, P. Dal Monte, and M. P. Landini. 2008. Remodelling of the nuclear lamina during human cytomegalovirus infection: role of the viral proteins pUL50 and pUL53. J. Gen. Virol. 89:731-740.
3. Dal Monte, P., S. Pignatelli, N. Zini, N. M. Maraldi, E. Ferret, M. C. Prevost, and M. P. Landini. 2002. Analysis of intracellular and intraviral localization of the human cytomegalovirus UL53 protein. J. Gen. Virol. 83:1005-1012.
4. Fuchs, W., B. G. Klupp, H. Granzow, N. Osterrieder, and T. C. Mettenleiter. 2002. The interacting UL31 and UL34 gene products of pseudorabies virus are involved in egress from the host-cell nucleus and represent components of primary enveloped but not mature virions. J. Virol. 76:364-378.
5. Gonnella, R., A. Farina, R. Santarelli, S. Raffa, R. Feederle, R. Bei, M. Granato, A. Modesti, L. Frati, H. J. Delecluse, M. R. Torrisi, A. Angeloni, and A. Faggioni. 2005. Characterization and intracellular localization of the Epstein-Barr virus protein BFLF2: interactions with BFRF1 and with the nuclear lamina. J. Virol. 79:3713-3727.
6. Granato, M., R. Feederle, A. Farina, R. Gonnella, R. Santarelli, B. Hub, A. Faggioni, and H. J. Delecluse. 2008. Deletion of Epstein-Barr virus BFLF2 leads to impaired viral DNA packaging and primary egress as well as to the production of defective viral particles. J. Virol. 82:4042-4051.
7. Klupp, B. G., H. Granzow, W. Fuchs, G. M. Keil, S. Finke, and T. C. Mettenleiter. 2007. Vesicle formation from the nuclear membrane is induced by coexpression of two conserved herpesvirus proteins. Proc. Natl. Acad. Sci. USA 104:7241-7246.
8. Lake, C. M., and L. M. Hutt-Fletcher. 2004. The Epstein-Barr virus BFRF1 and BFLF2 proteins interact and coexpression alters their cellular localiza tion. Virology 320:99-106.
9. Laurent, T. C., and J. Killander. 1964. A theory of gel filtration and its experimental verification. J. Chromatogr. 14:317-330.
10. Lötzerich, M., Z. Ruzsics, and U. H. Koszinowski. 2006. Functional domains of murine cytomegalovirus nuclear egress protein M53/p38. J. Virol. 80:73-84.
11. Mettenleiter, T. C. 2004. Budding events in herpesvirus morphogenesis. Virus Res. 106:167-180.
12. Mettenleiter, T. C. 2002. Herpesviras assembly and egress. J. Virol. 76:1537-1547.
13. Mettenleiter, T. C., B. G. Klupp, and H. Granzow. 2006. Herpesvirus assem bly: a tale of two membranes. Curr. Opin. Microbiol. 9:423-429.
14. Mettenleiter, T. C., and T. Minson. 2006. Egress of alphaherpesvirases. J. Virol. 80: 1610-1611. (Author reply, 80:1611-1612.)
15. Milbradt, J., S. Auerochs, and M. Marschall. 2007. Cytomegaloviral proteins pUL50 and pUL53 are associated with the nuclear lamina and interact with cellular protein kinase C. J. Gen. Virol. 88:2642-2650.
16. Mocarski, E. S. J., T. Shenk, and R. F. Pass. 2007. Cytomegaloviruses, p. 2701-2772. In M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Field's virology, 5th ed., vol. 1. Lippincott Williams and Wilkins, Philadelphia, PA.
17. Muranyi, W., J. Haas, M. Wagner, G. Krohne, and U. H. Koszinowski. 2002. Cytomegalovirus recruitment of cellular kinases to dissolve the nuclear lam ina. Science 297:854-857.
18. L34. J. Virol. 78:5564-5575.
19. L34 proteins of herpes simplex virus type 1 form a complex that accumulates at the nuclear rim and is required for envelopment of nucleocapsids. J. Virol. 75:8803-8817.
20. Rupp, B., Z. Ruzsics, C. Buser, B. Adler, P. Walther, and U. H. Koszinowski. 2007. Random screening for dominant-negative mutants of the cytomegalo virus nuclear egress protein M50. J. Virol. 81:5508-5517.
21. Schnee, M., Z. Ruzsics, A. Bubeck, and U. H. Koszinowski. 2006. Common and specific properties of herpesviras UL34/UL31 protein family members revealed by protein complementation assay. J. Virol. 80:11658-11666.
22. Shiba, C., T. Daikoku, F. Goshima, H. Takakuwa, Y. Yamauchi, O. Koiwai, and Y. Nishiyama. 2000. The UL34 gene product of herpes simplex virus type 2 is a tail-anchored type II membrane protein that is significant for virus envelopment. J. Gen. Virol. 81:2397-2405.
23. Villarreal, E. C. 2003. Current and potential therapies for the treatment of herpes-virus infections. Prog. Drag Res. 60:263-307.
24. Wiseman, T., S. Williston, J. F. Brandts, and L. N. Lin. 1989. Rapid mea surement of binding constants and heats of binding using a new titration calorimeter. Anal. Biochem. 179:131-137.
25. Yamauchi, Y., C. Shiba, F. Goshima, A. Nawa, T. Murata, and Y. Nishiyama. 2001. Herpes simplex virus type 2 UL34 protein requires UL31 protein for its relocation to the internal nuclear membrane in transfected cells. J. Gen. Virol. 82:1423-1428.