Development of a real-time PCR assay for the detection of Trichinella spiralis in situ

Veterinary Parasitology

Volumn 161, Issue 1-2, 2009, Pages 92-98

  Atterby, H ^a   Learmount, J ^a   Conyers, C ^a   Zimmer, I ^a   Boonham, N ^a   Taylor, M ^a  

^a CENTRAL SCIENCE LABORATORY   (United Kingdom)

Author keywords

Detection; Fox; Muscle; Real time PCR; Trichinella spiralis; Trichinellosis

Indexed keywords

DNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DILUTION; DNA EXTRACTION; FOX; GENOME; INFECTION RATE; ISOLATION PROCEDURE; LARVA; MOUSE; MUSCLE; NEMATODIASIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE IDENTIFICATION; REAL TIME POLYMERASE CHAIN REACTION; TRICHINELLA SPIRALIS; UNITED KINGDOM;

ANIMALS; FOXES; MICE; MUSCLE, SKELETAL; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRICHINELLA SPIRALIS;

ANIMALIA; CANIDAE; MAMMALIA; NEMATODA; SUIDAE; TRICHINELLA; TRICHINELLA SPIRALIS; VULPES;

EID: 63149103125     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2008.12.009     Document Type: Article

References (26)

1. Beck R., Gaspar A., Mihaljevic Z., Marinculic A., Stojcevic D., and Brstilo M. Evaluation of ELISA for detection of Trichinella antibodies in muscle juice samples of naturally infected pigs. Vet. Parasitol. 132 (2005) 91-95
2. Oldham J.N., and Beresford-Jones W.P. Trichinella spiralis in the wild red fox in England. Br. Vet. J. 113 (1957) 34-35
3. Boonham N., Fisher T., and Mumford R.A. Investigating the specificity of real-time PCR assays using synthetic oligonucleotides. J. Virol. Methods 130 (2005) 30-35
4. European Economic Community. Commision Directive 77/96/EEC. Off. J. Eur. Comm. 26 (2005) 67-77
5. Gamble H.R. Detection of Trichinellosis in pigs by artificial digestion and enzyme immunoassay. J. Food. Prot. 59 (1996) 295-298
6. Gamble H.R. Sensitivity of artificial digestion and enzyme immunoassay methods of inspection for trichinae in pigs. J. Food Prot. 61 (1998) 339-343
7. Gamble H.R., Pozio E., Bruschi F., Nöckler K., Kapel C.M.O., and Gajadhar A.A. International commission on trichinellosis: recommendations on the use of serological tests for the detection of Trichinella infection in animals and man. Parasite 11 (2004) 3-13
8. Kapel C.M.O., Oivanen L., La Rosa G., Mikkonen T., and Pozio E. Evaluation of two PCR-based techniques for molecular epidemiology in Finland, a high-endemic area with four sympatric Trichinella species. Parasite 8 (2001) S39-S43
9. Moller L.N., Petersen E., Gamble H.R., and Kapel C.M.O. Comparison of two antigens for demonstration of Trichinella spp. antibodies in blood and muscle fluid of foxes, pigs and wild boars. Vet. Parasitol. 132 (2005) 81-84
10. Muller N., Sager H., Schuppers M., and Gottstein B. Approaches to investigate Trichinella-infections in domestic and wild animals. Schweizer Archiv fur Tierheilkunde 148 (2006) 463-471
11. Mumford R.A., Walsh K., Barker I., and Boonham N. Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. Phytopathology 90 (2000) 448-453
12. Nöckler K., and Voigt W.P. Experimental Trichinella spiralis infection in the silver fox (Vulpes vulpes fulva). In: Ortega-Pierres G., Gamble H.R., van Knapen F., and Wakelin D. (Eds). Trichinellosis, Proceedings of the 9th International Conference on Trichinellosis (1998), Germar Press, Nonoalco Tlateloco, Mexico 319-323
13. Nöckler K., Pozio E., Voigt W.P., and Heidrich J. Detection of Trichinella infection in food animals. Vet. Parasitol. 93 (2000) 335-350
14. Nöckler K., Serrano F.J., Boireau P., Kapel C.M.O., and Pozio E. Experimental studies in pigs on Trichinella detection in different diagnostic matrices. Vet. Parasitol. 132 (2005) 85-90
15. Nöckler K., Reckinger S., and Pozio E. Trichinella spiralis and Trichinella pseudospiralis mixed infection in a wild boar (Sus scrofa) of Germany. Vet. Parasitol. 137 (2006) 364-368
16. Pozio E., and Zarlenga D.S. Recent advances on the taxonomy, systematics and epidemiology of Trichinella. Int. J. Parasitol. 35 (2005) 1191-1204
17. Rafter P., Marucci G., Brangan P., and Pozio E. Rediscovery of Trichinella spiralis in red foxes (Vulpes vulpes) in Ireland after 30 years of oblivion. J. Infect. 50 (2005) 61-65
18. Sambrook J., and Russell D.W. Preparation and Analysis of Eukaryotic Genomic DNA: Molecular Cloning: A Laboratory Manual. third ed. (2001), Cold Spring Harbor Laboratory Press, New York
19. Smith H.J. Differentiation of Trichinella spiralis spiralis and Trichinella spiralis nativa based on resistance to low temperature refrigeration. Can. J. Comp. Med. 47 (1983) 501-502
20. Vercammen F., Vervaeke M., Dorny P., Brandt J., Brochier B., Geerts S., and Verhagen R. Survey for Trichinella spp. in red foxes (Vulpes vulpes) in Belgium. Vet. Parasitol. 103 (2002) 83-88
21. Webster P., Maddox-Hyttel C., Nöckler K., Malakauskas A., van der Giessen J., Pozio E., Boireau P., and Kapel C.M.O. Meat inspection for Trichinella in pork, horsemeat and game within the EU: available technology and its present implementation. Eurosurveillance 11 (2006) 50-55
22. Weller S.A., Elphinstone J.G., Smith N.C., Boonham N., and Stead D.E. Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Appl. Environ. Microbiol. 66 (2000) 2853-2858
23. Zarlenga D.S., Chute M.B., Martin A., and Kapel C.M.O. A multiplex PCR for unequivocal differentiation of all encapsulated and non-encapsulated genotypes of Trichinella. Int. J. Parasitol. 29 (1999) 1859-1867
24. Zarlenga D.S., Chute M.B., Martin A., and Kapel C.M.O. A single, multiplex PCR for differentiating all species of Trichinella. Parasite-Journal de la Societe Francaise de Parasitologie 8 (2001) S24-S26
25. Zarlenga D.S., and La Rosa G. Molecular and biochemical methods for parasite differentiation within the genus Trichinella. Vet. Parasitol. 93 (2000) 279-292
26. Zimmer I.A., Hunter S.J., Morgan C.P., Hunt K.R., Smith G.C., Howall M., and Taylor M.A. Detection and surveillance for animal Trichinellosis in GB. Vet. Parasitol. 151 (2008) 233-241