Verification of the ProPneumo-1 assay for the simultaneous detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in clinical respiratory specimens

Annals of Clinical Microbiology and Antimicrobials

Volumn 8, Issue , 2009, Pages

  Higgins, Rachel R ^a   Lombos, Ernesto ^a   Tang, Patrick ^a   Rohoman, Karl ^a   Maki, Anne ^a   Brown, Shirley ^a   Jamieson, Frances ^a,b   Drews, Steven J ^a,b,c  

^a ONTARIO MINISTRY OF HEALTH AND LONG TERM CARE   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c MOUNT SINAI HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; NUCLEIC ACID;

ADOLESCENT; ADULT; AGED; ARTICLE; AUTOMATION; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CHILD; CHLAMYDIAL PNEUMONIA; CHLAMYDOPHILA PNEUMONIAE; CLINICAL EFFECTIVENESS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; FEMALE; HUMAN; INFANT; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOPLASMA PNEUMONIA; MYCOPLASMA PNEUMONIAE; REAL TIME POLYMERASE CHAIN REACTION; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; CHLAMYDOPHILA INFECTION; EVALUATION; ISOLATION AND PURIFICATION; MICROBIOLOGY; MIDDLE AGED; PRESCHOOL CHILD; RESPIRATORY TRACT INFECTION; SPUTUM;

ADOLESCENT; ADULT; AGED; CHILD; CHILD, PRESCHOOL; CHLAMYDOPHILA INFECTIONS; CHLAMYDOPHILA PNEUMONIAE; FEMALE; HUMANS; INFANT; MALE; MIDDLE AGED; MYCOPLASMA PNEUMONIAE; PNEUMONIA, MYCOPLASMA; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; SPUTUM; YOUNG ADULT;

EID: 63149091103     PISSN: None     EISSN: 14760711     Source Type: Journal    
DOI: 10.1186/1476-0711-8-10     Document Type: Article

References (20)

1. Stralin K Tornqvist E Kaltoft MS Olcen P Holmberg H Etiologic diagnosis of adult bacterial pneumonia by culture and PCR applied to respiratory tract samples J Clin Microbiol 2006, 44:643-645 1392682 16455935 10.1128/ JCM.44.2.643-645.2006
2. Loens K Ursi D Goossens H Ieven M Molecular diagnosis of Mycoplasma pneumoniae respiratory tract infections J Clin Microbiol 2003, 41:4915-4923 262541 14605118 10.1128/JCM.41.11.4915-4923.2003
3. Waites KB Talkington DF Mycoplasma pneumoniae and its role as a human pathogen Clin Microbiol Rev 2004, 17:697-728 523564 15489344 10.1128/ CMR.17.4.697-728.2004
4. Heath PT Epidemiology and bacteriology of bacterial pneumonias Paediatr Respir Rev 2000, 1:4-7 10.1053/prrv.2000.0001 16263435
5. McIntosh K Community-acquired pneumonia in children N Engl J Med 2002, 346:429-437 10.1056/NEJMra011994 11832532
6. Daxboeck F Krause R Wenisch C Laboratory diagnosis of Mycoplasma pneumoniae infection Clin Microbiol Infect 2003, 9:263-273 10.1046/ j.1469-0691.2003.00590.x 12667235
7. Loens K Ieven M Ursi D Beck T Overdijk M Sillekens P Goossens H Detection of Mycoplasma pneumoniae by real-time nucleic acid sequence-based amplification J Clin Microbiol 2003, 41:4448-4450 193797 12958290 10.1128/JCM.41.9.4448-4450.2003
8. Winchell JM Thurman KA Mitchell SL Thacker WL Fields BS Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation J Clin Microbiol 2008, 46:3116-3118 2546712 18614663 10.1128/JCM.00440-08
9. Dumke R Schurwanz N Lenz M Schuppler M Luck C Jacobs E Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach J Clin Microbiol 2007, 45:2726-2730 1951254 17537933 10.1128/JCM.00321-07
10. Gammie AJ For the proposition: For the diagnosis of viral infections, commercial assays provide more reliable results than do in-house assays Rev Med Virol 2008, 18:73-76 10.1002/rmv.564 18306442
11. James V Against the proposition: For the diagnosis of viral infections, commercial assays provide more reliable results than do in-house assays Rev Med Virol 2008, 18:77-80 10.1002/rmv.562 18306441
12. Tondella ML Talkington DF Holloway BP Dowell SF Cowley K Soriano-Gabarro M Elkind MS Fields BS et al Development and evaluation of real-time PCR-based fluorescence assays for detection of Chlamydia pneumoniae J Clin Microbiol 2002, 40:575-583 153405 11825973 10.1128/ JCM.40.2.575-583.2002
13. Ursi D Dirven K Loens K Ieven M Goossens H Detection of Mycoplasma pneumoniae in respiratory samples by real-time PCR using an inhibition control J Microbiol Methods 2003, 55:149-153 10.1016/ S0167-7012(03)00131-3 14500006
14. Loens K Ursi D Goossens H Ieven M Evaluation of the NucliSens miniMAG RNA extraction and real-time NASBA applications for the detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in throat swabs J Microbiol Methods 2008, 72:217-219 10.1016/j.mimet.2007.11.008 18077024
15. Drews SJ Blair J Lombos E DeLima C Burton L Mazzulli T Low DE Use of the Seeplex RV Detection kit for surveillance of respiratory viral outbreaks in Toronto, Ontario, Canada Ann Clin Lab Sci 2008, 38:376-379 18988931
16. Eshaghi A Blair J Burton L Choi KW De LC Duncan C Guyard C Higgins R Lombos E Low DE Mazzulli T Drews SJ Characterization of an influenza A and influenza B co-infection of a patient in a long-term care facility with co-circulating influenza A and influenza B Int J Infect Dis 2008, 18849179
17. Brice G Drews SJ Low DE Respiratory virus panels for global surveillance of emerging infectious diseases J Clin Virol 2007, 40:S58-S60 10.1016/ S1386-6532(07)70014-8 18162260
18. Boman J Gaydos CA Quinn TC Molecular diagnosis of Chlamydia pneumoniae by infection J Clin Microbiol 1999, 37:3791-3799 85816 10565886
19. Khanna M Fan J Pehler-Harrington K Waters C Douglass P Stallock J Kehl S Henrickson KJ The pneumoplex assays, a multiplex PCR-enzyme hybridization assay that allows simultaneous detection of five organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella micadadei, and Bordetella pertussis, and its real-time counterpart J Clin Microbiol 2005, 43:565-571 548062 15695646 10.1128/ JCM.43.2.565-571.2005
20. Ginevra C Baranger C Ros A Mory O Stephan J-L Freymuth F Joannes M Pozzeto B Grattard F Development and evaluation of chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chalmydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR J Clin Microbiol 2005, 43:3247-3254 1169163 16000443 10.1128/JCM.43.7.3247-3254.2005