Nanosecond pulse generator with variable pulse duration for the study of pulse induced biological effects

Proceedings of the 2008 IEEE International Power Modulators and High Voltage Conference, PMHVC

Volumn , Issue , 2008, Pages 61-64

  Kolb, J F ^a   Scarlett, S ^a   Cannone, J ^a   Zhuang, J ^a   Osgood, C ^a   Schoenbach, K H ^a   De Angelis, A ^b   Zeni, L ^c  

^a OLD DOMINION UNIVERSITY   (United States)

^b UNIVERSITY MEDITERRANEA OF REGGIO CALABRIA   (Italy)

^c SECOND UNIVERSITY OF NAPLES   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CALCIUM ALLOYS; CELL CULTURE; CELL MEMBRANES; DC GENERATORS; ELECTRIC FIELD EFFECTS; ENZYME ACTIVITY; FLUORESCENCE; ION EXCHANGE; ION EXCHANGE MEMBRANES; OPTOELECTRONIC DEVICES; SIGNAL GENERATORS; SWITCHES;

BIOLOGICAL EFFECTS; BLUMLEIN; BLUMLEIN LINES; CALCIUM INDICATORS; CALCIUM MOBILIZATIONS; CELLULAR MEMBRANES; CONFOCAL FLUORESCENT MICROSCOPIES; ELECTRIC FIELD STRENGTHS; ELECTRICAL PULSE; EXTRACELLULAR; IDEAL SWITCHES; INDIVIDUAL CELLS; INTRACELLULAR CALCIUMS; JURKAT CELLS; LEUKEMIA CELLS; MEMBRANE POTENTIALS; NANOSECOND PULSE GENERATORS; POWER MOSFET; PULSE DISTORTIONS; PULSE DURATIONS; SWITCH-ON; SWITCHED PULSE;

PULSE GENERATORS;

EID: 62949199156     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/IPMC.2008.4743577     Document Type: Conference Paper

References (13)

1. S. J. Beebe, P. M. Fox, L. J. Rec, K. Somers, R. H. Stark, K. H. Schoenbach, "Nanosecond, High-Intensity Pulsed Electric Fields Induce Apoptosis in Human Cells, FASEB J., vol. 11, pp. 1493-1495, 2003.
2. R. Nuccitelli et al, "Nanosecond Pulsed Electric Fields Cause Melanomas to Self-Destruct, Biochem. Biophys. Res. Commun., vol. 343, pp. 351-360, 2006.
3. X. Chen et al, "Apoptosis Pathway Study of Melanoma Treated by Nanosecond Pulsed Electric Field," 48th Annual National Student Research Forum, April 27-28, 2007, The University of Texas Medical Branch, Galveston, Texas, USA.
4. J. Zhang, P. F. Blackmore, B. Y. Hargrave, S. Xiao, S. J. Beebe, K. H. Schoenbach, "Nanosecond Pulse Electric Field (Nanopulse): A Novel Non-Ligand Agonist for Platelet Activation," Archives Biochem. Biophys., vol. 471, 240-248, 2008.
5. S. E. Buescher, private communication.
6. K. H. Schoenbach, F. E. Peterkin, R. W. Alden, and S. Beebe, "The effect of pulsed electric fields on biological cells: experiments and applications," IEEE Trans. Plasma Sci., vol. 25, pp. 284-292, 1997.
7. P. T. Vernier, Y. Sun, L. Marcu, S. Salemi, C. M. Craft, M. A. Gundersen, "Calcium Bursts Induced by Nanosecond Electric Pulses," Biochem. Biophys. Res. Commun., vol. 310, pp. 286-295, 2003.
8. Q. Hu, R. P. Joshi, K. H. Schoenbach, "Simulations of Nanopore Formation and Phosphatidylserine Externalization in Lipid Membranes Subjected to a High-Intensity, Ultrashort Electric Pulse," Phys Rev E Stat Nonlin Soft Matter Phys., vol. 72, pp. 031902-1 - 031902-10, 2005.
9. T. R. Gowrishanker, A. T. Esser, Z. Vasilkoski, K. C. Smith, J. C. Weaver, "Microdosimetry for Conventional and Supra-Electroporation in Cells with Organelles," Biochem. Biophys. Res. Commun., vol. 341, pp. 1266-1276,2006.
10. J. Deng, K. H. Schoenbach, E. S. Buescher, P. S. Hair, P. M. Fox, S. J. Beebe, "The Effects of Intense Submicrosecond Electrical Pulses on Cells," Biophys. J., vol. 84, pp. 2709-2714, 2003.
11. W. Frey et al., "Plasma Membrane Voltage Changes During Nanosecond Pulsed Electric Field Exposure" Biophys. J., vol. 90, pp. 3608-3615,2006.
12. A. de Angelis, J. F. Kolb, L. Zeni, K.H. Schoenbach, "Kilovolt Blumlein pulse generator with variable pulse duration and polarity," Rev. Sci. Instr., vol. 79, 2008, in print.
13. S. Shaka, J. A. White, P. F. Blackmore, K. H. Schoenbach, J. F. Kolb, "Temporal Resolved Calcium Response of Jurkat Cells to Nanosecond Pulsed Electric Fields," unpublished.