Specificity of staphylococcal phage and SaPI DNA packaging as revealed by integrase and terminase mutations

Molecular Microbiology

Volumn 72, Issue 1, 2009, Pages 98-108

  Ubeda, Carles ^a,d   Olivarez, Nicholas P ^b,c   Barry, Peter ^a   Wang, Huaibin ^a   Kong, Xiangpeng ^a   Matthews, Avery ^a   Tallent, Sandra M ^b   Christie, Gail E ^b   Novick, Richard P ^a  

^a NEW YORK UNIVERSITY MEDICAL CENTER   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^d MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; DNA; INTEGRASE; OLIGONUCLEOTIDE; TERMINASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; DNA PACKAGING; GENE FUNCTION; GENE INTERACTION; GENE MUTATION; GENE REPLICATION; GENETIC ANALYSIS; GENOME; GENOTYPE; NONHUMAN; PLASMID; PRIORITY JOURNAL; REPRODUCTION; SPECIES DIFFERENCE; STAPHYLOCOCCUS PHAGE; VIRUS CAPSID;

CAPSID; DNA PACKAGING; ENDODEOXYRIBONUCLEASES; GENOMIC ISLANDS; INTEGRASES; MUTATION; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS PHAGES; SUBSTRATE SPECIFICITY; VIRAL PROTEINS; VIRUS ASSEMBLY;

MIRIDAE; STAPHYLOCOCCUS AUREUS;

EID: 62949184210     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2009.06634.x     Document Type: Article

References (28)

1. Arnaud, M., Chastanet, A. Debarbouille, M. (2004) New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, Gram-positive bacteria. Appl Environ Microbiol 70 : 6887 6891.
2. de Azavedo, J.C., Foster, T.J., Hartigan, P.J., Arbuthnott, J.P., O'Reilly, M., Kreiswirth, B.N. Novick, R.P. (1985) Expression of the cloned toxic shock syndrome toxin 1 gene (tst) in vivo with a rabbit uterine model. Infect Immun 50 : 304 309.
3. Barry, P. (2006) Molecular genetics of the SaPI1 life cycle in Staphylococcus aureus. Doctoral dissertation, Department of Microbiology, New York University School of Medicine.
4. Bowden, D.W. Modrich, P. (1985) In vitro maturation of circular bacteriophage P2 DNA. Purification of ter components and characterization of the reaction. J Biol Chem 260 : 6999 7007.
5. Charpentier, E., Anton, A.I., Barry, P., Alfonso, B., Fang, Y. Novick, R.P. (2004) Novel cassette-based shuttle vector system for Gram-positive bacteria. Appl Environ Microbiol 70 : 6076 6085.
6. Fujisawa, H. Morita, M. (1997) Phage DNA packaging. Genes Cells 2 : 537 545.
7. Hirai, K. Fukasawa, T. (1976) Regional replication of the bacterial chromosome induced by derepression of prophage lambda. II. Direction and origin. Mol General Genet 147 : 71 78.
8. Lindqvist, B.H., Deho, G. Calendar, R. (1993) Mechanisms of genome propagation and helper exploitation by satellite phage P4. Microbiol Rev 57 : 683 702.
9. Lindsay, J.A., Ruzin, A., Ross, H.F., Kurepina, N. Novick, R.P. (1998) The gene for toxic shock toxin is carried by a family of mobile pathogenicity islands in Staphylococcus aureus. Mol Microbiol 29 : 527 543.
10. Maiques, E., Ubeda, C., Tormo, M.A., Ferrer, M.D., Lasa, I., Novick, R.P. Penades, J.R. (2007) Role of staphylococcal phage and SaPI Integrase in intra- and interspecies SaPI transfer. J Bacteriol 189 : 5608 5616.
11. Maniatis, T., Fritsch, E.F. Sambrook, J. (1982) Molecular Cloning - A Laboratory Manual. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory.
12. Novick, R.P. (1967) Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology 33 : 155 166.
13. Novick, R.P. (1991) Genetic systems in staphylococci. In : Methods in Enzymology (Bacterial Genetics). Miller, J. (ed.). Orlando, FL : Academic Press, pp. 587 636.
14. Novick, R.P., Edelman, I. Lofdahl, S. (1986) Small Staphylococcus aureus plasmids are transduced as linear multimers that are formed and resolved by replicative processes. J Mol Biology 192 : 209 220.
15. Novick, R.P., Ross, H.F., Projan, S.J., Kornblum, J., Kreiswirth, B. Moghazeh, S. (1993) Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. EMBO J 12 : 3967 3975.
16. Poliakov, A., Chang, J.R., Spilman, M.S., Damle, P.K., Christie, G.E., Mobley, J.A. Dokland, T. (2008) Capsid size determination by Staphylococcus aureus pathogenicity island SaPI1 involves specific incorporation of SaPI1 proteins into procapsids. J Mol Biol 380 : 465 475.
17. Ruzin, A., Lindsay, J. Novick, R.P. (2001) Molecular genetics of SaPI1 - a mobile pathogenicity island in Staphylococcus aureus. Mol Microbiol 41 : 365 377.
18. Sambrook, J., Fritsch, E.F. Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory.
19. Spanova, A. (1992) Comparison of permuted region lengths in the genomes of related Salmonella typhimurium phages P22 and L. Folia Microbiol 37 : 188 192.
20. Subedi, A., Ubeda, C., Adhikari, R.P., Penades, J.R. Novick, R.P. (2007) Sequence analysis reveals genetic exchanges and intraspecific spread of SaPI2, a pathogenicity island involved in menstrual toxic shock. Microbiology 153 : 3235 3245.
21. Tallent, S.M., Langston, T.B., Moran, R.G. Christie, G.E. (2007) Transducing particles of Staphylococcus aureus pathogenicity island SaPI1 Are comprised of helper phage-encoded proteins. J Bacteriol 189 : 7520 7524.
22. Tormo, M.A., Ferrer, M.D., Maiques, E., Ubeda, C., Selva, L., Lasa, I., et al. (2008) SaPI DNA is packaged in large as well as in small capsids, both composed of phage proteins. J Bacteriol 190 : 2434 2440.
23. Úbeda, C. (2005) Islas de patogenicidad de Staphylococcus aureus: ¿una nueva familia de elementos genéticos móviles diseñados para ser eficientements replicados y transferidos horizontalmente? Doctoral dissertation, Universidad de Cardinal Herrera-CEU, Valencia.
24. Ubeda, C., Tormo, M.A., Cucarella, C., Trotonda, P., Foster, T.J., Lasa, I. Penades, J.R. (2003) Sip, an integrase protein with excision, circularization and integration activities, defines a new family of mobile Staphylococcus aureus pathogenicity islands. Mol Microbiol 49 : 193 210.
25. Ubeda, C., Maiques, E., Knecht, E., Lasa, I., Novick, R.P. Penades, J.R. (2005) Antibiotic-induced SOS response promotes horizontal dissemination of pathogenicity island-encoded virulence factors in staphylococci. Mol Microbiol 56 : 836 844.
26. Ubeda, C., Maiques, E., Novick, R.P. Penades, J.R. (2007a) SaPI operon I is required for packaging and is regulated by LexA. Mol Microbiol 65 : 41 50.
27. Ubeda, C., Penades, J.R. Novick, R.P. (2007b) A pathogenicity island replicon in Staphylococcus aureus replicates as an unstable plasmid. Proc Nat Acad Sci USA 104 : 14182 14188.
28. Ubeda, C., Barry, P., Novick, R.P. Penades, J.R. (2008) Characterization of mutations defining SaPI functions and enabling autonomous replication in the absence of helper phage. Mol Microbiol 67 : 493 503.