Improved procedure for nucleus extraction for DNA measurements by flow cytometry of red beet (Beta vulgaris L.) hairy roots

Journal of Bioscience and Bioengineering

Volumn 107, Issue 4, 2009, Pages 439-441

  Georgiev, Vasil ^a   Weber, Jost ^b,c   Bley, Thomas ^b   Pavlov, Atanas ^a  

^a Department of Microbial Biosynthesis and Biotechnologies   (Bulgaria)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^c Partec GmbH   (Germany)

Author keywords

DNA profile; Flow cytometry; Hairy root; Nuclei extraction; Red beet

Indexed keywords

BETA VULGARIS; DNA MEASUREMENTS; FLOW CYTOMETRIC MEASUREMENTS; GERMANY; HAIRY ROOT; NUCLEI EXTRACTION; RED BEET;

CENTRIFUGATION; DNA; FLOW CYTOMETRY; GENES;

NUCLEIC ACIDS;

BETA VULGARIS; CELL NUCLEUS; DNA, PLANT; FLOW CYTOMETRY; INDICATORS AND REAGENTS; PLANT LEAVES; PLANT ROOTS; RHIZOBIUM;

BETA VULGARIS; BETA VULGARIS SUBSP. VULGARIS;

EID: 62949162455     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2008.12.023     Document Type: Article

References (16)

1. Zrÿd P., and Christinet L. Betalains. In: Davies K. (Ed). Plant Pigments and Their Manipulation. Annu. Plant Rev. vol. 14 (2004), CRC Press/Blackwell Publ, Oxford, U.K. 185-213
2. Allegra M., Furtmuller P.G., Jantschko W., Zederbauer M., Tesoriere L., Livrea M.A., and Obinger C. Mechanism of interaction of betanin and indicaxanthin with human myeloperoxidase and hypochlorous acid. Biochem. Biophys. Res. Commun. 332 (2005) 837-844
3. Galati E.M., Mondello M.R., Lauriano E.R., Taviano M.F., Galluzzo M., and Miceli N. Opuntia ficus indica (L.) Mill. fruit juice protects liver from carbon tetrachloride-induced injury. Phytother. Res. 19 (2005) 796-800
4. Kapadia G.J., Azuine M.A., Sridhar R., Okuda Y., Tsuruta A., Ichiishi E., Mukainake T., Takasaki M., Konoshima T., Nishino H., and Tokuda H. Chemoprevention of DMBA-induced UV-B promoted, NOR-1-induced TPA promoted skin carcinogenesis, and DEN-induced phenobarbital promoted liver tumors in mice by extract of beetroot. Pharmacol. Res. 47 (2003) 141-148
5. Pavlov A., Georgiev V., and Ilieva M. Betalain biosynthesis by red beet (Beta vulgaris L.) hairy root culture. Process Biochem. 40 (2005) 1531-1533
6. Edahiro J., Yamada M., Seike S., Kakigi Y., Miyanaga K., Nakamura M., Kanamori T., and Seki M. Separation of cultured strawberry cells producing anthocyanins in aqueous two-phase system. J. Biosci. Bioeng. 100 (2005) 449-454
7. Rao S.R., and Ravishankar G.A. Plant cell cultures: chemical factories of secondary metabolites. Biotechnol. Adv. 20 (2002) 101-153
8. Aird E.L.H., Hamill J.D., and Rhodes M.J.C. Cytogenetic analysis of hairy root cultures from a number of plant species transformed by Agrobacterium rhizogenes. Plant Cell, Tissue Organ Cult. 15 (1988) 47-57
9. Greilhuber J., Temsch E.M., and Loureiro J.C.M. Nuclear DNA content measurement. In: Dolezel J., Greilhuber J., and Suda J. (Eds). Flow Cytometry with Plant Cells (2007), Wiley & Sons, New York 67-99
10. Hendrix B., and Stewart J.M. Estimation of the nuclear DNA content of Gossypium species. Ann. Bot. (London) 95 (2005) 789-797
11. Dolezel J., Greilhuber J., and Suda J. Flow cytometry with plants: an overview. In: Dolezel J., Greilhuber J., and Suda J. (Eds). Flow Cytometry with Plant Cells (2007), Wiley & Sons, New York 41-66
12. Pavlov A., Kovatcheva P., Georgiev V., Koleva I., and Ilieva M. Biosynthesis and radical scavenging activity of betalains during the cultivation of red beet (Beta vulgaris) hairy root cultures. Z. Naturforsch., C Biosci. 57 (2002) 640-644
13. Loureiro J., Rodriguez E., Dolezel J., and Santos C. Comparison of four nuclear isolation buffers for plant DNA flow cytometry. Ann. Bot. (London) 98 (2006) 679-689
14. Arumuganathan K., and Earle E.D. Nuclear DNA content of some important plant species. Plant Mol. Biol. Rep. 9 (1991) 208-218
15. Dolezel J., Binarova P., and Lucretti S. Analysis of nuclear DNA content in plant cells by flow cytometry. Biol. Plant 31 (1989) 113-120
16. Noirot M., Barre C., Duperray C., Luoarn J., and Hamon S. Effects of caffeine and chlorogenic acid on propidium iodide accessibility to DNA: consequences on geneome size evaluation in coffee tree. Ann. Bot. (London) 92 (2003) 259-264