The amino-terminal region of Atg3 is essential for association with phosphatidylethanolamine in Atg8 lipidation

FEBS Letters

Volumn 583, Issue 7, 2009, Pages 1078-1083

  Hanada, Takao ^a   Satomi, Yoshinori ^b   Takao, Toshifumi ^b   Ohsumi, Yoshinori ^a  

^a NATIONAL INSTITUTE FOR BASIC BIOLOGY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Atg3; Atg8 PE; Autophagosome; Autophagy; Phosphatidylethanolamine; Ubiquitin like protein

Indexed keywords

PHOSPHATIDYLETHANOLAMINE;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTOPHAGY; CONTROLLED STUDY; GENE MUTATION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

AUTOPHAGY; LYSOSOMES; MICROTUBULE-ASSOCIATED PROTEINS; PHOSPHATIDYLETHANOLAMINES; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; UBIQUITIN; UBIQUITIN-CONJUGATING ENZYMES; VACUOLES;

EUKARYOTA;

EID: 62849120511     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2009.03.009     Document Type: Article

References (11)

1. Ohsumi Y. Molecular dissection of autophagy: two ubiquitin-like systems. Nat. Rev. Mol. Cell Biol. 2 (2001) 211-216
2. Ichimura Y., Imamura Y., Emoto K., Umeda M., Noda T., and Ohsumi Y. In vivo and in vitro reconstitution of Atg8 conjugation essential for autophagy. J. Biol. Chem. 279 (2004) 40584-40592
3. Hanada T., Noda N.N., Satomi Y., Ichimura Y., Fujioka Y., Takao T., Inagaki F., and Ohsumi Y. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem. 282 (2007) 37298-37302
4. Nakatogawa H., Ichimura Y., and Ohsumi Y. Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion. Cell 130 (2007) 165-178
5. Kirisako T., Baba M., Ishihara N., Miyazawa K., Ohsumi M., Yoshimori T., Noda T., and Ohsumi Y. Formation process of autophagosome is traced with Apg8/Aut7p in yeast. J. Cell Biol. 147 (1999) 435-446
6. Xie Z., Nair U., and Klionsky D.J. Atg8 controls phagophore expansion during autophagosome formation. Mol. Biol. Cell 19 (2008) 3290-3298
7. Yamada Y., Suzuki N.N., Hanada T., Ichimura Y., Kumeta H., Fujioka Y., Ohsumi Y., and Inagaki F. The crystal structure of Atg3, an autophagy-related ubiquitin carrier protein (E2) enzyme that mediates Atg8 lipidation. J. Biol. Chem. 282 (2007) 8036-8043
8. Noda T., Matsuura A., Wada Y., and Ohsumi Y. Novel system for monitoring autophagy in the yeast Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 210 (1995) 126-132
9. Oh-oka K., Nakatogawa H., and Ohsumi Y. Physiological pH and acidic phospholipids contribute to substrate specificity in lipidation of Atg8. J. Biol. Chem. 283 (2008) 21847-21852
10. Scaglione K.M., Bansal P.K., Deffenbaugh A.E., Kiss A., Moore J.M., Korolev S., Cocklin R., Goebl M., Kitagawa K., and Skowyra D. SCF E3-mediated autoubiquitination negatively regulates activity of Cdc34 E2 but plays a nonessential role in the catalytic cycle in vitro and in vivo. Mol. Cell Biol. 27 (2007) 5860-5870
11. Mishra V.K., Palgunachari M.N., Segrest J.P., and Anantharamaiah G.M. Interactions of synthetic peptide analogs of the class A amphipathic helix with lipids. Evidence for the snorkel hypothesis. J. Biol. Chem. 269 (1994) 7185-7191