Added value of combined gene and protein expression of CK20 and CEA in non-macroscopically involved lymph nodes of colorectal cancer

International Journal of Surgical Pathology

Volumn 17, Issue 2, 2009, Pages 93-98

  D'Armento, Giuseppe ^a   Daniele, Lorenzo ^a   Mariani, Sara ^a   Ottaviani, Davide ^b   Mussa, Antonio ^a   Cassoni, Paola ^a   Sapino, Anna ^a   Bussolati, Gianni ^a  

^a UNIVERSITY OF TURIN   (Italy)

^b UNIVERSITY OF TURIN   (Italy)

Author keywords

Colorectal cancer; Lymph node micrometastases; Messenger RNA integrity; Methacarn fixation

Indexed keywords

CARCINOEMBRYONIC ANTIGEN; CYTOKERATIN 2; MESSENGER RNA;

ARTICLE; CANCER CELL; CANCER STAGING; CLINICAL ARTICLE; COLORECTAL ADENOMA; COLORECTAL CANCER; CONTROLLED STUDY; GENE EXPRESSION; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; LYMPH NODE METASTASIS; MESENTERY LYMPH NODE; MICROMETASTASIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENTINEL LYMPH NODE;

ADENOMA; CARCINOEMBRYONIC ANTIGEN; CASE-CONTROL STUDIES; COLORECTAL NEOPLASMS; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; IMMUNOHISTOCHEMISTRY; KERATIN-20; LYMPH NODES; LYMPHATIC METASTASIS; LYMPHOCYTES; NEOPLASM STAGING; RNA, MESSENGER; TUMOR MARKERS, BIOLOGICAL;

EID: 62849106040     PISSN: 10668969     EISSN: None     Source Type: Journal    
DOI: 10.1177/1066896908328574     Document Type: Article

References (20)

1. Jestin P., Pahlman L., Glimelius B., Gunnarsson U. Cancer staging and survival in colon cancer is dependent on the quality of the pathologists' specimen examination. Eur J Cancer. 2005 ; 41: 2071-2078.
2. Lassmann S., Bauer M., Rosenberg R., et al. Identification of occult tumor cells in node negative lymph nodes of colorectal cancer patients by cytokeratin 20 gene and protein expression. Int J Colorectal Dis. 2004 ; 19: 87-94.
3. Swanson PE HIERanarchy: the state of the art in immunohistochemistry. Am J Clin Pathol. 1997 ; 107: 139-140.
4. Doekhie FS, Kuppen PJ, Peeters KC, et al. Prognostic relevance of occult tumour cells in lymph nodes in colorectal cancer. Eur J Surg Oncol. 2006 ; 32: 253-258.
5. Calaluce R., Miedema BW, Yesus YW Micrometastasis in colorectal carcinoma: a review. J Surg Oncol. 1998 ; 67: 194-202.
6. Bilchik AJ, Saha S., Wiese D., et al. Molecular staging of early colon cancer on the basis of sentinel node analysis: a multicenter phase II trial. J Clin Oncol. 2001 ; 19: 1128-1136.
7. Wong CS, Cheung MT, Ma BB, et al. Isolated tumor cells and circulating CK20 mRNA in pN0 colorectal cancer patients. Int J Surg Pathol. 2008 ; 16: 119-126.
8. Turner RR, Nora DT, Trocha SD, Bilchik AJ Colorectal carcinoma nodal staging: frequency and nature of cytokeratin-positive cells in sentinel and nonsentinel lymph nodes. Arch Pathol Lab Med. 2003 ; 127: 673-679.
9. Jung R., Kruger W., Hosch S., et al. Specificity of reverse transcriptase polymerase chain reaction assays designed for the detection of circulating cancer cells is influenced by cytokines in vivo and in vitro. Br J Cancer. 1998 ; 78: 1194-1198.
10. Rosenberg R., Friederichs J., Gertler R., et al. Prognostic evaluation and review of immunohistochemically detected disseminated tumor cells in peritumoral lymph nodes of patients with pN0 colorectal cancer. Int J Colorectal Dis. 2004 ; 19: 430-437.
11. Liefers GJ, Cleton-Jansen AM, ven de Veld CJ, et al. Micrometastases and survival in stage II colorectal cancer. N Engl J Med. 1998 ; 339: 223-228.
12. Shibutani M., Uneyama C., Miyazaki K., et al. Methacarn fixation: a novel tool for analysis of gene expressions in paraffin-embedded tissue specimens. Lab Invest. 2000 ; 80: 199-208.
13. Bussolati G., Chiusa L., Cimino A., D'Armento G. Tissue transfer to pathology labs: under vacuum is the safe alternative to formalin. Virchows Arch. 2008 ; 452: 229-231.
14. Daniele L., Annaratone L., Allia E., et al. Technical limits of comparison of step-sectioning, immunohistochemistry and RT-PCR on breast cancer sentinel nodes: a study on methacarn fixed tissue. J Cell Mol Med. 2008 Jul 30. [Epub ahead of print]
15. Puchtler H., Barton BP, Waldrop FS, et al. A comparative study of myosins and prekeratin in epithelial cells of methacarn-fixed tissues. Histochemistry. 1985 ; 82: 403-409.
16. Ishida H., Miwa H., Tatsuta M., et al. Ki-67 and CEA expression as prognostic markers in Dukes' C colorectal cancer. Cancer Lett. 2004 ; 207: 109-115.
17. Hernandez BY, Frierson HF, Moskaluk CA, et al. CK20 and CK7 protein expression in colorectal cancer: demonstration of the utility of a population-based tissue microarray. Hum Pathol. 2005 ; 36: 275-281.
18. Cronin M., Pho M., Dutta D., et al. Measurement of gene expression in archival paraffin-embedded tissues: development and performance of a 92-gene reverse transcriptase-polymerase chain reaction assay. Am J Pathol. 2004 ; 164: 35-42.
19. Faguet GB Mechanisms of lymphocyte activation: binding kinetics of phytohemagglutinin to human lymphocytes. J Biol Chem. 1977 ; 252: 2095-2100.
20. Harper JT, Puchtler H., Meloan SN, Terry MS Light-microscopic demonstration of myoid fibrils in renal epithelial, mesangial and interstitial cells. J Microsc. 1970 ; 91: 71-85.