Haematopoietic culture systems

Bioreactors for Tissue Engineering: Principles, Design and Operation

Volumn , Issue , 2005, Pages 309-334

  Safinia, L ^a   Panoskaltsis, N ^a   Mantalaris, A ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 62849092401     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/1-4020-3741-4_14     Document Type: Chapter

References (144)

1. Abboud CN, Lichtman MA. 2001. Structure of the Marrow and the Hemopoietic Microenvironment. In: Seligsohn U, editor. Hematology. New York: McGraw-Hill. p 29-58
2. Aiuti A, Friedrich C, Sieff C, Gutierrez-Ramos J. 1998. Identification of distinct elements of the stromal microenvironment that control human hematopoietic stem/progenitor cell growth and differentiation. Experimental Hematology 26:143-157
3. Akatov V, Lezhnev, EI., Vexler, AM., Kublik, LN. 1985. Low pH value of pericellular medium as a factor limiting cell proliferation in dense cultures. Experimental Cell Research 160:412-418
4. Alley C, MacDermott, RP., Stewart, CC. 1983. The Effect of Serum on Human Marrow Mononuclear Cell Proliferation and Maturation. Journal of the Reticuloendothelial Society 34:271-278
5. Almeida-Porada G, Brown, RL., MacKintosh, FR., Zanjani, ED. 2000. Evaluation of Serum-Free Culture Conditions Able to Support the Ex Vivo Expansion and Engraftment of Human Hematopoietic Stem CElls in Human-To Sheep Xenograft Model. Journal of Hematotherapy & Stem Cell Research 9:683-693
6. Audet J, Zanstra, PW., Eaves, CJ., Piret, JM. 1998. Advances in hematopoietic stem cell culture. Current Opinion in Biotechnology 9:146-151
7. Bachier CR, Gokmen E, Teale J, Lanzkron S, Childs C, Franklin W, Shpall E, Douville J, Weber S, Muller T and others. 1999. Ex-vivo expansion of bone marrow progenitor cells for hematopoietic reconstitution following high-dose chemotherapy for breast cancer. Experimental Hematology 27:615-623
8. Bagley J, Rosenzweig, M., Mark, DF., Pykett, MJ. 1999. Extended culture of multipotent hematopoietic progenitors without cytokine augmentation in a novel three-dimensional device. Experimental Hematology 27:496-504
9. Barnett MJ, Eaves CJ, Phillips GL, Gascoyne RD, Hogge DE, Horsman DE, Humphries RK, Klingemann HG, Lansdorp PM, Nantel SH and others. 1994. Autografting with cultured marrow in chronic myeloid leukemia: Results of a pilot study. Blood 84:724-732
10. Bhatia M, Bonnet D, Wu D, Murdoch B, Wrana J, Gallacher L, Dick JE. 1999. Bone morphogenetic proteins regulate the developmental program of human hematopoietic stem cells. Journal of Experimental Medicine 189:1139-1148
11. Bhatia M, McGlave PB, Dewald GW, Blazar BR, Verfaillie CM. 1995. Abnormal function of the bone marrow microenvironment in chronic myelogenous leukemia: Role of malignant stromal macrophages. Blood 85:3636-3645
12. Bohmer R. 1989. Interaction of Serum and Colony-Stimulating Factor for Survival of a Factor-Dependent Hemopoietic Progenitor Cell Line. Journal of Cellular Physiology 139:531-537
13. Brandt J, Bhalla, K., Hoffman, R. 1994. Effects of interleukin-3 and c-kit ligand on the survival of various classes of human hematopoietic progenitor cells. Blood 83(6):1507-1514
14. Bregni M, Magni M, Siena S, Di Nicola M, Bonadonna G, Gianni AM. 1992. Human peripheral blood hematopoietic progenitors are optimal targets of retroviral-mediated gene transfer. Blood 80:1418-1422
15. Broudy VC. 1997. Stem cell factor and hematopoiesis. Blood 90:1354-1364
16. Brown R, Xu, FS., Dusing, SK., Li, Q., Fischer, R., Patchen, M. 1997. Serum-Free Conditions for Cells Capable of Producing Long-Term Survival in Lethally Irradiated Mice. Stem Cells 15:237-245
17. Broxmeyer HE, Sherry B, Cooper S, Lu L, Maze R, Beckmann MP, Cerami A, Ralph P. 1993. Comparative analysis of the human macrophage inflammatory protein family of cytokines (chemokines) on proliferation of human myeloid progenitor cells. Interacting effects involving suppression, synergistic suppression, and blocking of suppression. Journal of Immunology 150:3448-3458
18. Brugger W, Mocklin W, Heimfeld S, Berenson RJ, Mertelsmann R, Kanz L. 1993. Ex vivo expansion of enriched peripheral blood CD34+ progenitor cells by stem cell factor, interleukin-1 (IL-1), IL-6, IL-3, interferon g and erythropoietin. Blood 81:2579-2584
19. Cabral JMS. 2001. Ex vivo expansion of hematopoietic stem cells in bioreactors. Biotechnology Letters 23:741-751
20. Cairo MS, Wagner JE. 1997. Placental and/or umbilical cord blood: An alternative source of hematopoietic stem cells for transplantation. Blood 90:4664-4671
21. Caldwell J, Locey B, Clarke MF, Emerson SG, Palsson BO. 1991a. Influence of medium exchange schedules on metabolic, growth, and GM-CSF secretion rates of genetically engineered NIH-3T3 cells. Biotechnology Progress 7:1-8
22. Caldwell J, Palsson BO, Locey B, Emerson SG. 1991b. Culture perfusion schedules influence the metabolic activity and granulocyte-macrophage colony-stimulating factor production rates of human bone marrow stromal cells. Journal of Cellural Physiology 147:344-353
23. Carswell K, Papoutsakis, ET. 2000. Extracellular pH affects the proliferation of cultured human T-cells and their expression of the interleukin-2 receptor. Journal of Immunology 23(6):669-674
24. Chabannon C, Olivero S, Blaise D, Maraninchi D, Viens P. 2000. Ex vivo expansion of human hematopoietic progenitors and cells to support high-dose chemoradiation therapy: Five years of clinical experience. Cytokines, Cellular & Molecular Therapy 6:97-108
25. Charbord P. 2001. Microenvironmental Cell Populations Essential for the Support of Hematopoietic Stem Cells. In: Zon LI, editor. Hematopoiesis: A Developmental Approach. New York: Oxford University Press. p 691-701
26. Chute JP, Saini AA, Kampen RL, Wells MR, Davis TA. 1999. A comparative study of the cell cycle status and primitive cell adhesion molecule profile of human CD34+ cells cultured in stroma-free versus porcine microvascular endothelial cell cultures. Experimental Hematology 27:370-379
27. Cipolleschi M, D'Ippolito, G., Bernabei, PA., Caporale, R., Nannini, R., Mariani, M., Fabbiani, M., Rossi-Ferrini, P., Olivotto, M., Sbarba, PD. 1997. Severe hypoxia enhances the formation of erythroid bursts from human cord blood cells and the maintenance of BFU-E in vitro. Experimental Hematology 25:1187-1194
28. Cipolleschi MG, Dello Sbarba P, Olivotto M. 1993. The role of hypoxia in the mainetance of hematopoietic stem cells. Blood 82:2031-2037
29. Civin CI, Almeida-Porada G, Lee MJ, Olweus J, Terstappen LW, Zanjani ED. 1996. Sustained, retransplantable, multilineage engraftment of highly purified adult human bone marrow stem cells in vivo. Blood 88 4102-4109
30. Collins P, Miller, WM., Papoutsakis, ET. 1996. Ex vivo culture systems for hematopoietic cells. Current Opinion in Biotechnology 7(2):223-230
31. Collins PC, Miller ME, Papoutsakis ET. 1998. Stirred culture of peripheral and cord blood hematopoietic cells offers advantages over traditional static systems for clinically relevant applications. Biotechnology & Bioengineering 59:534-543
32. Coutinho LH, Testa NG, Chang J, Morgenstern G, Harrison C, Dexter TM. 1990. The use of cultured bone marrow cells in autologous transplantation. Progress in Clinical and Biological Research 333:415-432
33. Craig W, Kay R, Cutler RL, Lansdorp PM. 1993. Expression of Thy-1 on human hematopoietic progenitor cells. Journal of Experimental Medicine 177:1331-1342
34. Dao MA, Hashimo K, Kato I, Nolta JA. 1998. Adhesion to fibronectin maintains regenarative capacity during ex vivo culture and transduction of human hematopoietic stem and progenitor cells. Blood 92:4612-4621
35. De La Selle V, Gluckman E, Bruley-Rosset M. 1996. Newborn blood can engraft adult mice without inducing graft versus host disease across non H-2 antigens. Blood 87:3977-3984
36. Dexter TM, Allen TD, Lajtha LG. 1977. Conditions controlling the proliferation of haematopoietic stem cells in vivo. Journal of Cellular Physiology 91:335-344
37. Douay L. 2001. Experimental Culture Conditions Are Critical for Ex Vivo Expansion of Hematopoietic Cells. Journal of Hematotherapy & Stem Cell Research 10:341-346
38. Dybedal I, Jacobsen SE. 1995. Transforming growth factor beta (TGF-beta), a potent inhibitor of erythropoiesis: Neutralizing TGF-beta antibodies show erythropoietin as a potent stimulator of murine burst-forming unit erythroid colony formation in the absence of a burst-promoting activity. Blood 86:949-957
39. Eaves CJ, Cashman JD, Kay RJ, Dougherty GJ, Otsuka T, Gaboury LA, Hogge DE, Lansdorp PM, Eaves AC, Humphries RK. 1991. Mechanisms that regulate the cell cycle status of very primitive hematopoietic cells in long-Term human marrow cultures. II. Analysis of positive and negative regulators produced by stromal cells within the adherent layer. Blood 78:110-117
40. Eaves CJ, Eaves AC. 1997. Stem cell kinetics. Baillieres Clinical Haematology 10:233-257
41. Emmons RV, Doren S, Zujewski J, Cottler-Fox M, Carter CS, Hines K, O'Shaughnessy JA, Leitman SF, Greenblatt JJ, Cowan K and others. 1997. Retroviral gene transduction of adult peripheral blood or marrow-derived CD34+ cells for six hours without growth factors or on autologous stroma does not improve marking efficiency assessed in vivo. Blood 89:4040-4046
42. Endo T, Ishibashi, Y., Okana, H., Fukumaki, Y. 1994. Sgnificance of pH on differentiation of human erythroid cell lines. Leukemia Research 18:49-54
43. Erslev A, et al. 1983. Structure and function of the marrow. al. WWe, editor: New York: McGraw-Hill. 75-83 p.
44. Fischkoff S, Pollak, A., Gleich, GJ., Testa, JR., Misawa, S., Reber, TJ. 1984. Eosinophilic differentiation of the human promyelocytic leukemia cell line. Journal of Experimental medicine 160:179-196
45. Fortunel N, Hatzfeld, A. and Hatzfeld, JA. 2000. Transforming growth factor-B: Pleiotropic role in the regulation of hematopoiesis. Blood 96(6):2022-2036
46. Gartner S, Kaplan, HS. 1980. Long-Term culture of human bone marrow cells. Proc. Natl. Acad. Sci. (USA) 77:4756-4759
47. Giarratana M-C, Kobari, L., Neildez Nguyen, TMA., Firat, H., Bouchet, S., Lopez, M., Gorin, N-C., Thierry, D., Douay, L. 1998. Cell culture bags allow a large extent of ex vivo expansion of LTC-IC and functional mature cells which can subsequently be frozen: Interest for large-scale clinical appplication. Bone Marrow Transplantation 22(7):707-715
48. Goodell M, Brose K, Paradis G, Conner A, Mulligan A. 1996. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. Journal of Experimental Medicine 183:1797-1806
49. Guba S, Gottschalk, LR., Jing, YH., Mulligan, T., Emerson, SG. 1992. Bone marrow stromal fibroblasts secrete interleukin-6 and granulocyte-macrophage colony-stimulating factor in the absence of inflammatory stimulation: Demonstration by serum-free bioassay, enzyme-linked immunosorbent assay, and reverse transcriptase polymerase chain reaction. Blood 80(5):1190-1198
50. Haylock DN, To LB, Dowse TL, Juttner CA, Simmons PJ. 1992. Ex vivo expansion and maturation of peripheral blood CD34+ cells into the myeloid lineage. Blood 80:1405-1412
51. Hevehan D, Papoutsakis, ET., Miller, WM. 2000. Physiologically significant effect of pH and oxygen tension on granulopoiesis. Experimental Hematology 28:267-275
52. Highfill J, Haley, SD., Kompala, DS. 1996. Large-scale production of murine bone marrow cells in an airlift packed bed bioreactor. Biotechnology and Bioengineering 50:514-520
53. Holyoake TL, Freshney MG, McNair L, Parker AN, McKay PJ, Steward WP, Fitzsimons E, Graham GJ, Pragnell IB. 1996. Ex vivo expansion with stem cell factor and interleukin-11 augments both shortterm recovery posttransplant and the ability to serially transplant marrow. Blood 87:4589-4595
54. Horner M, Miller ME, Ottino JM, Papoutsakis ET. 1998. Transport in a grooved perfusion flat-bed bioreactor for cell therapy applications. Biotechnology Progress 14:689-698
55. Ishikawa Y, Ito, T. 1988. Kinetics of hematopietic stem cells in a hypoxic culture. European Journal of Haematology 40:126-129
56. Ivanovic Z, Bartolozzi, B., Bernabei, PA., et al. 2000. Incubation of murine bone marrow cells in hypoxia ensures the maintenance of marrow-repopulating ability together with the expansion of commited progenitors. British Journal of Haematology 108:424-429
57. Ivanovic Z, Belloc, F., Faucher, J., et al. 2002. Hypoxia maintains and interleukin-3 reduces the precolony-forming cell potential of dividing CD34+ murine bone marrow. Experimental Hematology 30:67-73
58. Jeevarajan A. 2002. Continuous pH Monitoring in a Perfused Bioreactor System Using an Optical pH Sensor. Biotechnology AND Bioengineering 78(4):467-472
59. Kanai M, Hirayama, F., Yamaguchi, M., Ohkawara, J., Sato, N., Fukazawa, K., Yamashita, K., Kuwabara, M., Ikeda, H. & Ikebuchi, K. 2000. Stromal cell-dependent ex vivo expansion of human cord blood and augmentation of transplantable stem cell activity. Bone Marrow Transplantation 26:837-844
60. Karanu FN, Murdoch B, Gallacher L, Wu DM, Koremoto M, Sakano S, Bhatia M. 2000. The notch ligand jagged-1 represents a novel factor of human hematopoietic stem cells. Journal of Experimental Medicine 192:1365-1372
61. Kim D, Fujiki, Y., Fukushima, T., Ema, H., Shibuya, A., Nakauchi, H. 1999. Comparison of Hematopoietic Activities of Human Bone Marrow and Umbilical Cord Blood CD34 Positive and Negative Cells. Stem Cells 17:286-294
62. Kobayashi M, Laver JH, D LS, Kato T, Miyazaki H, Ogawa M. 1996. Thrombopoietin supports proliferation of human primitive hematopoietic cells in synergy with steel factor and/or interleukin-3. Blood 88:429-436
63. Kobayashi M, Laver JH, Lyman SD, Kato T, Miyazaki H, Ogawa M. 1997. Thrombopoietin, steel factor and the ligand for flt3/flk2 interact to stimulate the proliferation of human hematopoietic progenitors in culture. International. Journal of Hematology 66:423-434
64. Koller M, Bender, JG., Papoutsakis, ET. & Miller, WM. 1992a. Effects of synergistic cytokine combinations, low oxygen, and irradiated stroma on the expansion of human cord blood progenitors. Blood 80(2):403-411
65. Koller M, Emerson, SG. & Palsson, BO. 1993. Large-Scale Expansion of Human Stem and Progenitor Cells From Bone Marrow Mononuclear Cells in Continuous Perfusion Cultures. Blood 82(2):378-384
66. Koller M, et al. 1992b. Reduced Oxygen Tension Increases Hematopoiesis in Long-Term Culture of Human Stem and Progenitor Cells from Cord Blood and Bone Marrow. Experimental Hematology 20:264-270
67. Koller M, Manchel, I., Maher, RJ., Goltry, KL., Armostrong, D., Smith, AK. 1998. Clinical-Scale Human Umbilical Cord Blood Cell Expansion in a Novel Automated Perfusion Culture System. Bone Marrow Transplantation 21:653-663
68. Koller M, Manchel, I., Palsson, MA., Maher, RJ. 1996. Different Measures of Human Hematopoietic Cell Culture Performance Are Optimized under Vastly Different Conditions. Biotechnology and Bioengineering 50:505-513
69. Koller MR, Bender JG, Miller ME, Papoutsakis ET. 1993. Expansion of primitive human hematopoietic progenitors in a perfusion bioreactor system with IL-3, IL-6, and stem cell factor. Bio/Technology 11:358-63
70. Koller MR, Bradley TR, Palsson BO. 1995a. Growth factor consumption and production in perfusion cultures of human bone marrow correlate with specific cell production. Experimental Hematology 23:1275-1283
71. Koller MR, Manchel I, Newsom BS, Palsson MA, Palsson BO. 1995b. Bioreactor expansion of human bone marrow: Comparison of unprocessed, density-separated and CD34-enriched cells. Journal of Hematotherapy 4:159-169
72. Koller MR, Oxender M, Jensen TC, Goltry KL, Smith AK. 1999. Direct contact between CD34+/Lincells and stroma induces a soluble activity that specifically increases primitive hematopoietic cell production. Experimental Hematology 27:734-741
73. Krause DS, Fackler MJ, Civin CI, May WS. 1996. CD34: Structure, biology, and clinical utility. Blood 87:1-13
74. Krystal G, Lanm V, Dragowska W, Takahashi C, Appel J. 1994. Transforming growth factor beta 1 is an inducer of erythroid differentiation. Journal of Experimental Medicine 180:851-860
75. Lansdorp PD, W. 1992. Long-Term Erythropoiesis from Constant Numbers of CD34+ Cells in Serumfree Cultures initiated with Highly Purified Progenitor Cells from Human Bone Marrow. Journal of Experimental Medicine 175:1501-1509
76. Larochelle A, Vormoor J, Hanenberg H, Wang JC, Bhatia M, Lapidot T, Moritz T, Murdoch B, Xiao XL, Kato I and others. 1996. Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: Implications for gene therapy. Nature Medicine 2:1329-1337
77. Lill MC, Lynch M, Fraser JK, Chung GY, Schiller G, Glaspy JA, Souza L, Baldwin GC, Gasson JC. 1994. Production of functional myeloid cells from CD34-selected hematopoietic progenitor cells using a clinically relevant ex vivo expansion system. Stem Cells 12:626-637
78. Lyman SD, Jacobsen SE. 1998. c-kit ligand and Flt3 ligand: Stem/progenitor cell factors with overlapping yet distinct activities. Blood 91:1101-1134
79. Lyman SD, Williams DE. 1995. Biology and potential clinical applications for flt3 ligand. Current Opinion in Hematology 2:177-181
80. Madlambayan GJ, Rogers I, Casper RF, Zandstra PW. 2001. Controlling Culture Dynamics for the Expansion of Hematopoietic Stem Cells. Journal of Hematotherapy & Stem Cell Research 10:481-492
81. Mantalaris A, Keng P, Bourne P, Chang AYC, Wu JHD. 1998. Engineering a human bone marrow model: A case study on ex vivo erythropoiesis. Biotechnology Progress 14:126-133
82. Martiat P, Ferrant, A., Cogneau, M., Bol, A., Rodhain, J., Michaux, JL., Sokal, G. 1987. Assessment of bone marrow blood flow using positron emmission tomography: No relationship with bone cellularity. Brithish Journal of Haematology 66:307-310
83. Martinson J, Unverzagt, K., Schaeffer, A., Smith, SL., Loudovaris, M., Schneidkraut, MJ., Bender, JG. and Van Epps, DE. 1998. Neutrophil Precursor Generation: Effect of Culture Conditions. Journal of Hematotherapy 7:463-471
84. Matsunaga T, Hirayama F, Yonemura Y, Murray R, Ogawa M. 1998. Negative regulation by interleukin-3 (IL-3) of mouse early B-cell progenitors and stem cells in culture: Transduction of the negative signals by betac and betaIL-3 proteins of IL-3 receptor and absence of negative regulation by granulocyte-macrophage colony-stimulating factor. Blood 92:901-907
85. Mayani H. 1996. Composition and function of the hematopoietic microenvironment in human myeloid leukemia. Leukemia 10:1041-1047
86. Mayani HaL, PM. 1998. Biology of Human Umbilical Cord Blood-Derived Hematopoietic Stem/Progenitor Cells. Srem Cells 16:153-165
87. McAdams T, Miller, WM. & Papoutsakis, ET. 1996. Hematopoietic cell culture therapies (part I): Cell culture considerations. Trends in Biotechnology 14:341-349
88. McAdams T, Sandstrom, CE., Miller, WM., Bender, JG. and Paputsakis, ET. 1995. Ex vivo expansion of primitive hematopoietic cells for cellular therapies: An overview. Cytotechnology 18:133-146
89. McAdams TA, Miller WM, Papoutsakis ET. 1997. Variations in culture pH affect the cloning eficiency and differentiation of progenitor cells in ex vivo haematopoiesis. British Journal of Haematology 97:889-895
90. McAdams TA, Miller WM, Papoutsakis ET. 1998. pH is a potent modulator of erythroid differentiation. British Journal of Haematology 103:317-325
91. McCune JM, Namikawa R, Kaneshima H, Shultz LD, Lieberman M, Weissman IL. 1998. The SCID-hu mouse: Murine model for the analysis of human hematolymphoid differentiation and function. Science 24:1632-1639
92. McDowell CL, Papoutsakis ET. 1998. Increased agitation intensity increases CD13 receptor surface content and mRNA levels, and alters the metabolism of HL60 cells cultures in stirred tank bioreactors. Biotechnology & Bioengineering 60:239-250
93. McNiece I, Briddell R. 2001. Ex vivo expansion of hematopoietic progenitor cells and mature cells. Experimental Hematology 29:3-11
94. Meyer C, Drexler HG. 1999. FLT3 ligand inhibits apoptosis and promotes survival of myeloid leukemia cell lines. Leukemia Lymphoma 32:577-581
95. Mobest D, Goan, S., Junghahn, I., Winkler, J., Fichtner, I., Becker, M., De Lima-Hahn, E., Mertelsmann, R. & Henschler, R. 1999. Differential Kinetics of Primitive Hematopoietic Cells Assayed In Vitro and In Vivo During Serum-Free Suspension Culture of CD34+ Blood Progenitor Cells. Stem Cells 17:152-161
96. Mobest D, Mertelsmann, R. & Henschler, R. 1998. Serum-Free ex vivo Expansion of CD34+Hematopoietic Progenitor Cells. Biotechnology and Bioengineering 60(3):341-347
97. Mostafa S, Miller, WM & Papoutsakis, ET. 2000. Oxygen tension influences the differentiation, maturation and apoptosis of human megakaryocytes. British Journal of Haematology 111:879-889
98. Naldini A, Carraro, F., Silvestri, S., et al. 1997. Hypoxia Affects Cytokine Production and Proliferation Responses by Human Peripheral Mononuclear Cells. Journal of Cellular Physiology 173:335-342
99. Naughton BA, Jacob L, Naughton GK. 1990. A three-dimensional culture system for the growth of hematopoietic cells. Progress in Clinical and Biological Research 333:435-445
100. Naughton BA, Naughton GK. 1989. Hematopoiesis on nylon mesh templates-comparative long-Term bone-marrow culture and the influence of stromal support cells. Annals of the New York Academy of Sciences 554:125-140
101. Naughton BA, Tjota A, Sibanda B, Naughton GK. 1991. Hematopoiesis on suspended nylon screenstromal cell microenvironments. Journal of Biomechanical Engineering-Transactions of the ASME 113:171-177
102. Nielsen LK. 1999. Bioreactors for Hematopoietic Cell Culture. Annual Reviews in Biomedical Engineering 1:129-152
103. Noll T, Jelinek, N., Schmidt, S., Biselli, M. & Wandrey, C. 2002. Cultivation of Hematopoietic Stem and Progenitor cells: Biochemical Engineering Aspects. Advances in Biochemical Engineering 74:111-128
104. Ogawa M, Clark SC. 1988. Synergistic interaction between interleukin-6 and interleukin-3 in support of stem cell proliferation in culture. Blood Cells 14:329-335
105. Oh D, Koller, MR. & Palsson, BO. 1994. Frequent Harvesting from Perfused Bone Marrow Cultures Results in in Increased Overall Cell and Progenitor Expansion. Biotechnology and Bioengineering 44:609-616
106. Ohmizono Y, Sakaba H, Kimura T, Tanimukai S, Matsumura T, Miyazaki H, Lyman SD, Sonoda Y. 1997. Thrombopoietin augments ex vivo expansion of human cord blood-derived hematopoietic progenitors in combination with stem cell factor and flt3 ligand. Leukemia 11:524-530
107. Osawa M, Hanada K, Hamada H, Nakauchi H. 1996. Long-Term lymphohematopoietic reconstitution by CD34-low/negative hematopoietic stem cells. Science 273:242-245
108. Palsson BO, Paek S-H, Schwartz RM, Palsson MA, Lee G-M, Silver S, Emerson SG. 1993. Expansion of human bone marrow progenitor cells in a high cell density continuous perfusion system. Bio/Technology 11:368-372
109. Pennathur-Das RL, L. 1987. Augmentation of In Vitro Human Marrow Erythropoiesis Under Physiological Oxygen Tensions Is Mediated by Monocytes and T Lymphocytes. Blood 69(3):899-907
110. Petzer AL, Zandstra PW, Piret JM, Eaves CJ. 1996. Differential cytokine effects on primitive (CD34+CD38-) human hematopoietic cells: Novel responses to flt3-ligand and thrombopoietin. Journal of Experimental Medicine 183:2551-2558
111. Piacibello W, Sanavio F, Garetto L, Severino A, Dane A, Gammaitoni I, Agglietta M. 1998. Differential growth factor requirement of primitive cord blood hematopoietic stem cells for self-renewal and amplification versus proliferation and differentiation. Leukemia 12:718-727
112. Pierson BA, Europa AF, Hu WS, Miller JS. 1996. Production of human natural killer cells for adoptive immunotherapy using a computer-controlled stirred-Tank bioreactor. Journal of Hematotherapy 5:474-483
113. Poloni A, Giarratana MC, Kobari L, Firat H, Bouchet S, Gorin NC, Douay L. 1997. The ex vivo expansion capacity of normal human bone marrow cells is dependent on experimental conditions: Role of cell concentration, serum and CD34+ cell selection in stroma-free cultures. Hematological Cellular Therapy 39:49-58
114. Quesenberry P, Colvin GA. 2001. Hematopoietic stem cells, progenitor cells, and cytokines. In: Seligsohn U, editor. Hematology. New York: McGraw-Hill. p 153-174
115. Quesenberry P, Crittenden R, Lowry P, Kittler E, Rao S, Peters S, Ramshaw H, Stewart F. 1994. In vitro and in vivo studies of stromal niches. Blood Cells 20:97-106
116. Rich I. 1986. A Role for the Macrophage in Normal Hemopoiesis. II. Effect of Varying Physiology Oxygen Tensions on the Release of Hemopoietic Growth Factors from Bone-marrow-derived Macrophage in vitro. Experimental Hematology 14:746-751
117. Rich I, & Kubanek, B. 1982. The effect of reduced oxygen tension on colony formation of erythropoietic cells in vitro. British Journal of Haematology 52:579-588
118. Rosenzwajg M, Canque B, Gluckman JC. 1996. Human dendritic cell differentiation pathway from CD34+ hematopoietic precursor cells. Blood 87:535-544
119. Rosenzweig M, Pykett MJ, Marks DF, Johnson RP. 1997. Enhanced maintenance and retroviral transduction of primitive hematopoietic progenitor cells using a novel three-dimensional culture system. Gene Therapy 4:928-936
120. Rusten LS, Smeland EB, Jacobsen FW, Lien E, Lesslauer W, Loetscher H, Dubois CM, Jacobsen SE. 1994. Tumor necrosis factor-Alpha inhibits stem cell factor-induced proliferation of human bone marrow progenitor cells in vitro. Role of p55 and p75 tumor necrosis factor receptors. Journal of Clinical Investigation 94:165-172
121. Sandstrom CE, Bender JG, Miller ME, Papoutsakis ET. 1996. Development of a novel perfusion chamber to retain nonadherent cells and its use for comparison of human mobilized peripheral blood mononuclear cell cultures with and without irradiated bone marrow stroma. Biotechnology & Bioengineering 50:493-504
122. Sandstrom CE, Bender JG, Papoutsakis ET, Miller WM. 1995. Effects of CD34+ cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells. Blood 86:958-970
123. Sardonini CA, Wu YJ. 1993. Expansion and differentiation of human hematopoietic cells from static cultures through small-scale bioreactors. Biotechnology Progress 9:131-137
124. Schwartz R, Palsson B, Emerson S. 1991. Rapid medium perfusion rate significantly increases the productivity and longevity of human bone marrow cultures. Proc. Natl. Acad. Sci. (USA) 88:6760-6764
125. Shah AJ, Smogorzewska EM, Hannum C, Crooks GM. 1996. Flt3 ligand induces proliferation of quiescent human bone marrow CD34+CD38-cells and maintains progenitor cells in vitro. Blood 87:3563-3570
126. Shih C-C, DiGiusto D, Forman SJ. 2002. Ex Vivo Expansion of Transplantable Human Hematopoietic Stem Cells: Where Do We Stand in the Year 2000? Journal of Hematotherapy & Stem Cell Research 9:621-628
127. SpangruDe GJ, Johnson GR. 1990. Resting and activated subsets of mouse multipotent hematopoietic stem cells. Proceedings of the National Academy of Sciences USA 87:7433-7477
128. Sutherland HJ, Lansdorp PM, Henkelman DH, Eaves AC, Eaves CJ. 1990. Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layers. Proceedings of the National Academy of Sciences USA 87:3584-3588
129. Tennant G. 2000. Control of pH in human long-Term bone marrow cultures with low-glucose medium containing zwitterion buffer lengthens the period of haematopoiesis activity. Brithish Journal of Haematology 109:785-787
130. Till JE, McCulloch EA. 1961. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiation Research 14:213-222
131. Trentin JJ. 1970. Influence of hematopoietic organ stroma (hematopoietic inductive microenvironments) on stem cell differentiation. In: Gordon AS, editor. Regulation of hemopoiesis. New York: Appleton-Century-crofts. p 161-186
132. Tsukada J, Misago, M., Kikuchi, M., Sato, T., Ogawa, R., Ota, T., Oda, S., Morimoto, I., Chiba, S. & Eto, S. 1992. Interaction between recombinant human erythropoietin and serum factor(s) on murine megakaryocyte colony formation. Blood 80(1):37-45
133. Veiby OP, Jacobsen FW, Cui L, Lyman SD, Jacobsen SE. 1996. The flt3 ligand promotes the survival of primitive hemopoietic progenitor cells with myeloid as well as B lymphoid potential. Suppression of apoptosis and counteraction by TNF-Alpha and TGF-beta. Journal of Immunology 157:2953-2960
134. Verfaillie CM. 2001. Ex Vivo Expansion of Hematopoietic Stem Cells. In: Zon LI, editor. Hematopoiesis: A Developmental Approach. New York: Oxford University Press. p 119-129
135. Verfaillie CM, Hurley R, Bhatia R, McCarthy JB. 1994. MIP-1a combined with IL-3 conserves primitive human LTBMC-IC for at least 8 weeks in ex vivo stroma-non-contact cultures. Journal of Experimental Medicine 179:643-649
136. Wang T-Y, Brennan JK, Wu JHD. 1995. Multilineal hematopoiesis in a three-dimensional murine longterm bone marrow culture. Experimental Hematology 23:26-32
137. Weissman I. 1994. Developmental switches in the immune system. Cell 76:207-218
138. Williams DE, Hangoc G, Cooper S, Boswell HS, Shadduck RK, Gillis S, Waheed A, Urdal D, Broxmeyer HE. 1987. The effects of purified recombinant murine interleukin-3 and/or purified natural murine CSF-1 in vivo on the proliferation of murine hifg-And low-proliferative potential colony-forming cells: Demonstration of in vivo synergism. Blood 70:401-403
139. Won JH, Cho SD, Park SK, Lee GT, Baick SH, Suh WS, Hong DS, Park HS. 2000. Thrombopoietin in synergy with other cytokines for expansion of cord blood progenitor cells. Journal of Hematotherapy and Stem Cell Research 9:465-473
140. Yagi M, Ritchie KA, Sitnicka E, Storey C, Roth GI, Bartelmez S. 1999. Sustained ex vivo expansion of hematopoietic cells mediated by thrombopoietin. Proceedings of the National Academy of Sciences USA 96:8126-8131
141. Yamaguchi M, Hiramaya, F., Kanai, M., Sato, N., Fukazawa, K., Yamashita, K., Sawada, K., Koike, T., Kuwabara, M., Ikeda, H. & Ikebuchi, K. 2001. Serum-free coculture system for ex vivo expansion of human cord blood primitive progenitos and SCID mouse-reconstituting cells using human bone marrow primary stromal cells. Experimental Hematology 29:174-182
142. Yang H, Miller, WM., Papoutsakis, ET. 2002. High pH promotes megakaryocytic maturation and apoptosis. Stem Cells 20:320-328
143. Zandstra PW, Conneally E, Petzer AL, Piret JM, Eaves CJ. 1997. Cytokine manipulation of primitive human hematopoietic cell self-renewal. Proceedings of the National Academy of Sciences USA 94:4698-4703
144. Zandstra PW, Eaves CJ, Piret JM. 1994. Expansion of Hematopoietic Progenitor Cell Populations in Stirred Suspension Bioreactors of Normal Human bone Marrow Cells. Bio/Technology 12:909-914