Simultaneous detection and quantitation of cytomegalovirus, Epstein-Barr virus, and adenovirus by use of real-time PCR and pooled standards

Journal of Clinical Microbiology

Volumn 47, Issue 3, 2009, Pages 765-770

  Gunson, R N ^a   Maclean, A R ^a   Shepherd, S J ^a   Carman, W F ^a  

^a GARTNAVEL GENERAL HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS; ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS; EPSTEIN BARR VIRUS; MIXED INFECTION; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; VIRUS DETECTION; VIRUS INFECTION;

ADENOVIRIDAE; CYTOMEGALOVIRUS; DNA PRIMERS; HERPESVIRUS 4, HUMAN; HUMANS; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

ADENOVIRIDAE; CYTOMEGALOVIRUS; HUMAN HERPESVIRUS 4;

EID: 62749155792     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01721-08     Document Type: Article

References (25)

1. Bonon, S. H., S. M. Menoni, C. L. Rossi, C. A. De Souza, A. C. Vigorito, D. B. Costa, and S. C. Costa. 2005. Surveillance of cytomegalovirus infection in haematopoietic stem cell transplantation patients. J. Infect. 50:130-137.
2. Cirino, N. M., N. P. Tavakoli, S. Madison-Anetnucci, and C. Egan. 2007. Multiplex RT-PCR in microbiology, p. 183-221. In I. M. Mackay (ed.), Real time PCR in microbiology: from diagnosis to characterisation. Caister Academic Press, Norwich, United Kingdom.
3. CLSI. 2006. Molecular diagnostic methods for infectious diseases: approved guideline, 2nd ed. CSLI document MM3-A2. Clinical and Laboratory Standards Institute, Wayne, PA.
4. CLSI. 2008. Verification and validation of multiplex nucleic acid assays; approved guideline. CSLI document MM17-A. Clinical and Laboratory Standards Institute, Wayne, PA.
5. Coleman, J. W., J. E. Johnson, and D. K. Clarke. 2007. Simultaneous quantification of four RNA targets by multiplex, real-time RT-PCR without optimization. BioTechniques 43:369-371.
6. Compston, L. I., F. Sarkobie, C. Li, D. Candotti, O. Opare-Sem, and J. P. Allain. 2008. Multiplex real-time PCR for the detection and quantification of latent and persistent viral genomes in cellular or plasma blood fractions. J. Virol. Methods 151:47-54.
7. Engel, H., C. Kueppers, M. Koenig, and D. Loeffert. 2007. Successful gene expression analysis by multiplex, real-time, one-step RT-PCR, irrespective of the targets amplified. BioTechniques 43:230-231.
8. Feuchtinger, T., P. Lang, and R. Handgretinger. 2007. Adenovirus infection after allogeneic stem cell transplantation. Leuk. Lymphoma 48:244-255.
9. Garson, J. A., P. R. Grant, U. Ayliffe, R. B. Ferns, and R. S. Tedder. 2005. Real-time PCR quantitation of hepatitis B virus DNA using automated sample preparation and murine cytomegalovirus internal control. J. Virol. Methods 126:207-213.
10. Ghaffari, S. H., N. Obeidi, M. Dehghan, K. Alimoghaddam, A. Gharehbaghian, and A. Ghavamzadeh. 8 April 2008. Monitoring of cytomegalovirus reactivation in bone marrow transplant recipients by real-time PCR. Pathol. Oncol. Res. doi:10.1007/s12253-008-9030-3.
11. Gunson, R., G. Gillespie, and W. F. Carman. 2003. Optimisation of PCR reactions using primer chessboarding. J. Clin. Virol. 26:369-373.
12. Gunson, R. N., T. C. Collins, and W. F. Carman. 2006. Practical experience of high throughput real time PCR in the routine diagnostic virology setting. J. Clin. Virol. 35:355-367.
13. Gunson, R. N., S. Bennett, A. Maclean, and W. F. Carman. 2008. Using multiplex real time PCR in order to streamline a routine diagnostic service. J. Clin. Virol. 43:372-375.
14. Heim, A., C. Ebnet, G. Harste, and P. Pring-Akerblom. 2003. Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J. Med. Virol. 70:228-239.
15. Ishii, T., H. Sootome, L. Shan, and K. Yamashita. 2007. Validation of universal conditions for duplex quantitative reverse transcription polymerase chain reaction assays. Anal. Biochem. 362:201-212.
16. Ishii, T., H. Sootome, and K. Yamashita. 2007. Practical evaluation of universal conditions for four-plex quantitative PCR. Anal. Bioanal. Chem. 388:271-278.
17. Kimura, H., Y. Ito, R. Suzuki, and Y. Nishiyama. 2008. Measuring Epstein-Barr virus (EBV) load: the significance and application for each EBV-associated disease. Rev. Med. Virol. 18:305-319.
18. Mackay, I. (ed.). 2007. Real time PCR in microbiology: from diagnosis to characterisation. Caister Academic Press, Norwich, United Kingdom.
19. Meerbach, A., P. Wutzler, R. Häfer, F. Zintl, and B. Gruhn. 2008. Monitoring of Epstein-Barr virus load after hematopoietic stem cell transplantation for early intervention in post-transplant lymphoproliferative disease. J. Med. Virol. 80:441-454.
20. Najioullah, F., D. Thouvenot, and B. Lina. 2001. Development of a real-time PCR procedure including an internal control for the measurement of HCMV viral load. J. Virol. Methods 92:55-64.
21. Niesters, H. G., J. van Esser, E. Fries, K. C. Wolthers, J. Cornelissen, and A. D. Osterhaus. 2000. Development of a real-time quantitative assay for detection of Epstein-Barr virus. J. Clin. Microbiol. 38:712-715.
22. Persson, K., K. Hamby, and L. A. Ugozzoli. 2005. Four-color multiplex reverse transcription polymerase chain reaction - overcoming its limitations. Anal. Biochem. 344:33-42.
23. Ratcliff, R. M., G. Chang, T. Kok, and T. P. Sloots. 2007. Molecular diagnosis of medical viruses. Curr. Issues Mol. Biol. 9:87-102.
24. Wada, K., N. Kubota, Y. Ito, H. Yagasaki, K. Kato, T. Yoshikawa, Y. Ono, H. Ando, Y. Fujimoto, T. Kiuchi, S. Kojima, Y. Nishiyama, and H. Kimura. 2007. Simultaneous quantification of Epstein-Barr virus, cytomegalovirus, and human herpesvirus 6 DNA in samples from transplant recipients by multiplex real-time PCR assay. J. Clin. Microbiol. 45:1426-1432.
25. Wittwer, C. T., M. G. Herrmann, C. N. Gundry, and K. S. Elenitoba-Johnson. 2001. Real-time multiplex PCR assays. Methods 25:430-442.