Ion trapping with fast-response ion-selective microelectrodes enhances detection of extracellular ion channel gradients

Biophysical Journal

Volumn 96, Issue 4, 2009, Pages 1597-1605

  Messerli, Mark A ^a   Collis, Leon P ^a   Smith, Peter J S ^a  

^a MARINE BIOLOGICAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ACTIVATED POTASSIUM CHANNEL; ION CHANNEL; POTASSIUM ION; POTASSIUM;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CHO CELL; CONTROLLED STUDY; DIFFUSION; FLUORESCENCE IMAGING; ION MONITORING; LIQUID MEMBRANE; MICROELECTRODE; NONHUMAN; POTASSIUM TRANSPORT; ANIMAL; CHANNEL GATING; CHEMISTRY; COMPUTER SIMULATION; CRICETULUS; EXTRACELLULAR SPACE; HAMSTER; METABOLISM; MONTE CARLO METHOD; PATCH CLAMP; PHYSIOLOGY;

CRICETULUS GRISEUS;

ANIMALS; CELL MEMBRANE; CHO CELLS; COMPUTER SIMULATION; CRICETINAE; CRICETULUS; DIFFUSION; EXTRACELLULAR SPACE; ION CHANNEL GATING; MICROELECTRODES; MONTE CARLO METHOD; PATCH-CLAMP TECHNIQUES; POTASSIUM; POTASSIUM CHANNELS, CALCIUM-ACTIVATED;

EID: 62649152411     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2008.11.025     Document Type: Article

References (26)

1. Hille, B. 2001. Ion Channels of Excitable Membranes. Sinauer Associates, Sunderland, MA.
2. Roberts, W. M., and W. Almers. 1992. Patch voltage clamping with low-resistance seals: loose patch clamp. Methods Enzymol. 207:155-176.
3. Korchev, Y. E., Y. A. Negulyaev, C. R. W. Edwards, I. Vodyanoy, and M. J. Lab. 2000. Functional localization of single active ion channels on the surface of a living cell. Nat. Cell Biol. 2:616-619.
4. 2+ flux through individual N-type voltage-gated channels expressed in Xenopus oocytes. Cell Calcium. 34:499-509.
5. 2+ flux through individual muscle acetylcholine receptor channels. J. Gen. Physiol. 126:179-192.
6. Haugland, R. P. 2005. The Handbook: A Guide to Fluorescent Probes and Labelling Technologies. Invitrogen, Carlsbad, CA.
7. Messerli, M. A., K. R. Robinson, and P. J. S. Smith. 2006. Electrochemical sensor applications to the study of molecular physiology and analyte flux in plants. In Plant Electrophysiology. A. G. Volkov, editor. Springer, Berlin, Heidelberg, New York, pp. 73-107.
8. Smith, P. J. S., R. H. Sanger, and M. A. Messerli. 2007. Principles, development and applications of self-referencing electrochemical microelectrodes to the determination of fluxes at cell membranes. In Electrochemical Methods for Neuroscience. A. C. Michael and L. M. Borland, editors. CRC Press, Boca Raton, FL, pp. 373-405.
9. Smith, P. J. S., K. Hammar, D. M. Porterfield, R. H. Sanger, and J. R. Trimarchi. 1999. Self-referencing, non-invasive, ion selective electrode for single cell detection of trans-plasma membrane calcium flux. Microsc. Res. Tech. 46:398-417.
10. + selective microelectrodes with low electrical membrane resistance. Neurosci. Lett. 74:221-226.
11. Ujec, E., O. Keller, N. Křiž, V. Pavlík, and J. Machek. 1980. Low - impedance, coaxial, ion-selective, double-barrel microelectrodes and their use in biological measurements. J. Electroanal. Chem. 116:363-369. (Pubitemid 11207230)
12. 2+-selective microelectrodes suitable for in vitro extracellular recording. J. Neurophysiol. 96:919-924.
13. Messerli, M. A., P. J. S. Smith, R. C. Lewis, and K. R. Robinson. 2004. Chloride fluxes in lily pollen tubes: a critical reevaluation. Plant J. 40:799-812.
14. 2+ buffers. In Methods in Cell Biology: A Practical Guide to the Study of Calcium in Living Cells. R. Nuccitelli, editor. Academic Press, San Diego, pp. 3-29.
15. Stiles, J. R., D. Van Helden, T. M. Bartol, E. E. Salpeter, and M. M. Salpeter. 1996. Miniature endplate current rise times <100 μs from improved dual recordings can be modeled with passive acetylcholine diffusion from a synaptic vesicle. Proc. Natl. Acad. Sci. USA. 93:5747-5752. (Pubitemid 26190751)
16. Stiles, J. R., and T. M. Bartol. 2001. Monte Carlo methods for simulating realistic synaptic microphysiology using MCell. In Computation Neuroscience: Realistic Modeling for Experimentalists. E. De Schutter, editor. CRC Press, London, pp. 87-127.
17. Gray, P. T. A. 1987. Analysis of whole cell currents to estimate the kinetics and amplitude of underlying unitary events: relaxation and "noise" analysis. In Microelectrode Techniques. D. Ogden, editor. The Company of Biologists, Cambridge.
18. Messerli, M. A., E. D. Corson, and P. J. S. Smith. 2007. Measuring extracellular ion gradients from single channels with ion-selective microelectrodes. Biophys. J. 92:L52-L54. (Pubitemid 46536408)
19. + channel from rat skeletal muscle incorporated into planar bilayers. J. Gen. Physiol. 98:1025-1043.
20. Butler, A., S. Tsunoda, D. P. McCobb, A. Wei, and L. Salkoff. 1993. mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels. Science. 261:221-224.
21. Ha, T. S., S. Y. Jeong, S. -W. Cho, H. -k. Jeon, G. S. Roh, et al. 2000. Funtional characteristics of two BKCa channel variants differentially expressed in rat brain tissues. Eur. J. Biochem. 267:910-918.
22. Barrett, J. N., K. L. Magleby, and B. S. Pallotta. 1982. Properties of single calcium-activated potassium channels in cultured rat muscle. J. Physiol. 331:211-230.
23. Ammann, D. 1986. Ion-Selective Microelectrodes. Springer-Verlag, Berlin.
24. Bakker, E., P. Bühlmann, and E. Pretsch. 1997. Carrier-based ion-selective electrodes and bulk optodes. 1. General characteristics. Chem. Rev. 97:3083-3132.
25. Orme, F. W. 1969. Liquid ion-exchanger microelectrodes. In Glass Microelectrodes. M. Lavalleé, O. F. Schanne, and N. C. Hébert, editors. John Wiley & Sons, New York, pp. 376-395.
26. Tucker, J. L., R. Wen, and B. Oakley, II.. 1991. A deconvolution technique for improved estimation of rapid changes in ion concentration recorded with ion-selective microelectrodes. IEEE Trans. Biomed. Eng. 38:156-160.