Calcofluor fluorescence assay for wort β-glucan in a microplate format

Cereal Chemistry

Volumn 86, Issue 1, 2009, Pages 33-36

  Schmitt, Mark R ^a   Budde, Allen D ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE ASSAY; GLUCANS; MICROPLATE FORMATS;

EID: 62449334935     PISSN: 00090352     EISSN: None     Source Type: Journal    
DOI: 10.1094/CCHEM-86-1-0033     Document Type: Article

References (6)

1. ASBC. 2004. American Society of Brewing Chemists. Methods of Analysis, 9th Ed. Malt-4 (Extract), Wort-18 (β-glucan in congress wort by fluorescence). The Society: St. Paul, MN.
2. EBC. 2007. European Brewery Convention Analytica-EBC. Malt 4.16.3 High molecular weight β-glucan content of malt wort: spectrophotometric method. Wort 8.13.1. High molecular weight β-glucan content of wort: enzymatic method. Wort 8.13.2 High molecular weight β-glucan content of malt: fluorimetric method. Hans Carl Verlag: Nürnberg, Germany.
3. Izawa, M., Takashio, M., and Tamaki, T. 1996. Inhibitor of fluorescence reactions between Calcofluor and β-(1,3)(1,4)-D-glucan in beer and wort. J. Inst. Brew. 102:87-91.
4. Puchalski, M. M., Morra, M. J., and von Wandruszka, R. 1991. Assessment of inner filter effect corrections in fluorimetry. Fresenius J. Anal. Chem. 340:341-344.
5. Riis, P. 2005. A new method for detection of β-glucan. Proc. Congr. Eur. Brew. Conv. 30:974-978.
6. Schmitt, M. R., Budde. A. D., and Marinac, L. A. 2006. Research mash: A simple procedure for conducting Congress mashes using reduced quantities of malt. J. Am. Soc. Brew. Chem. 64:181-186.