Nonlinear optical microscopy: Use of second harmonic generation and two-photon microscopy for automated quantitative liver fibrosis studies

Journal of Biomedical Optics

Volumn 13, Issue 6, 2008, Pages

  Sun, Wanxin ^a   Chang, Shi ^b,c   Tai, Dean C S ^a   Tan, Nancy ^c,d   Xiao, Guangfa ^b,c   Tang, Huihuan ^c   Yu, Hanry ^a,e,f  

^a INSTITUTE OF BIOENGINEERING AND NANOTECHNOLOGY   (Singapore)

^b CENTRAL SOUTH UNIVERSITY   (China)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d K K Women's and Children's Hospital   (Singapore)

^e SINGAPORE MIT ALLIANCE   (Singapore)

^f Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

Fibrillar collagen; Liver fibrosis; Quantification; Second harmonic generation; Two photon excited fluorescence

Indexed keywords

AUTOMATED IMAGE ANALYSIS; CELL MORPHOLOGY; CELL NECROSIS; CHRONIC LIVER DISEASE; CLINICAL APPLICATION; CLINICAL DIAGNOSTICS; COLLAGEN FIBER; EXTRACELLULAR MATRICES; FIBRILLAR COLLAGENS; HEPATOCYTES; LIMITED SENSITIVITY; LIVER FIBROSIS; LIVER TISSUE; NONLINEAR OPTICAL MICROSCOPY; QUANTIFICATION; QUANTITATIVE CHARACTERIZATION; QUANTITATIVE TOOL; SEMI-QUANTITATIVE; TWO PHOTON MICROSCOPY; TWO-PHOTON EXCITED FLUORESCENCE;

AUTOMATION; CELL DEATH; COLLAGEN; FLUORESCENCE; HARMONIC ANALYSIS; IMAGE ANALYSIS; MATHEMATICAL OPERATORS; MORPHOLOGY; NONLINEAR OPTICS; OPTICAL MICROSCOPY; PHOTONS; SENSITIVITY ANALYSIS; TISSUE;

HARMONIC GENERATION;

ALGORITHM; ANIMAL; ARTICLE; ARTIFICIAL INTELLIGENCE; AUTOMATED PATTERN RECOGNITION; COMPUTER ASSISTED DIAGNOSIS; EVALUATION; IMAGE ENHANCEMENT; IMAGE SUBTRACTION; LIVER CIRRHOSIS; MALE; METHODOLOGY; MULTIPHOTON MICROSCOPY; NONLINEAR SYSTEM; PATHOLOGY; RAT; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; WISTAR RAT;

ALGORITHMS; ANIMALS; ARTIFICIAL INTELLIGENCE; IMAGE ENHANCEMENT; IMAGE INTERPRETATION, COMPUTER-ASSISTED; LIVER CIRRHOSIS; MALE; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; NONLINEAR DYNAMICS; PATTERN RECOGNITION, AUTOMATED; RATS; RATS, WISTAR; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SUBTRACTION TECHNIQUE;

EID: 62449159329     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.3041159     Document Type: Article

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