Whole-mount in situ hybridization detection of mRNA in GFP-marked drosophila imaginal disc mosaic clones

Fly

Volumn 2, Issue 6, 2008, Pages 323-325

  VanZomeren Dohm, Adrienne ^a   Flannery, Ellen ^b   Duman Scheel, Molly ^a,b  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

Drosophila; GFP; Imaginal disc; In situ hybridization; Mosaic clone; MRNA detection

Indexed keywords

GREEN FLUORESCENT PROTEIN; MESSENGER RNA;

ANIMAL; ARTICLE; CELL CLONE; DROSOPHILA; IN SITU HYBRIDIZATION; METABOLISM; METHODOLOGY; MOSAICISM; PRENATAL DEVELOPMENT;

ANIMALS; CLONE CELLS; DROSOPHILA; GREEN FLUORESCENT PROTEINS; IN SITU HYBRIDIZATION; MOSAICISM; RNA, MESSENGER;

EID: 62149103014     PISSN: 19336934     EISSN: 19336942     Source Type: Journal    
DOI: 10.4161/fly.7230     Document Type: Article

References (8)

1. Tautz D, Pfeifle V. A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 1989; 98:81-5.
2. Xu Y, Rubin GM. Analysis of genetic mosaics in developing and adult Drosophila tissues. Development 1993; 117:1223-37.
3. Xu T, Harrison ST. Mosaic analysis using FLP recombinase. Methods Cell Biol 1994; 44:655-81.
4. Struhl G, Basler K. Organizing activity of wingless protein in Drosophila. Cell 1993; 72:527-40.
5. Pignoni F, Zipursky S. Induction of Drosophila eye development by decapentalegic. Development 1997; 124:271-8.
6. Patel NH. In situ hybridization to whole mount Drosophila embryos. In: Kreig PA, ed. A Laboratory Guide to RNA: Isolation, Analysis and Synthesis. New York: Wiley Liss 1996; 357-69.
7. Cohen B, Cohen SM. Double labeling of mRNA and proteins in Drosophila embryos. In: Kessler C, ed. Nonradioactive Labeling and Detection of Biomolecules. Berlin/Heidelberg: Springer-Verlag 1992; 382-92.
8. 1 arrest in the presumptive Drosophila wing margin. PNAS 2004; 101:3857-62.