Use of Comet-FISH in the study of DNA damage and repair: Review

Mutation Research - Reviews in Mutation Research

Volumn 681, Issue 1, 2009, Pages 33-43

  Glei, Michael ^a   Hovhannisyan, Galina ^b   Pool Zobel, Beatrice L ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b YEREVAN STATE UNIVERSITY   (Armenia)

Author keywords

Comet assay; Comet FISH; LT97; Primary human colon cells

Indexed keywords

4 HYDROXYNONENAL; BIOLOGICAL MARKER; FERRIC ION; HEXOBARBITAL; HYDROGEN PEROXIDE; IRON;

CARCINOGENESIS; CELL LINE; COLON CANCER; COMET ASSAY; DNA DAMAGE; DNA REPAIR; EVALUATION; FLUORESCENCE IN SITU HYBRIDIZATION; GENOTOXICITY; HISTORY; HUMAN; NONHUMAN; OXIDATIVE STRESS; PRACTICE GUIDELINE; PRIORITY JOURNAL; REVIEW; SENSITIVITY ANALYSIS; TELOMERE; TUMOR GENE;

CELL LINE, TUMOR; COLONIC NEOPLASMS; COMET ASSAY; DNA DAMAGE; DNA REPAIR; GENES, APC; GENES, P53; GENES, RAS; GENETIC MARKERS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MUTAGENS; OXIDATIVE STRESS; TELOMERE;

EID: 62149093577     PISSN: 13835742     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrrev.2008.01.006     Document Type: Review

References (88)

1. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., and Sasaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35 (2000) 206-221
2. Baatout S., and Derradji H. Cytometric methods to analyze radiation effects. J. Biol. Regul. Homeost. Agents 18 (2004) 101-105
3. Huber R., Kulka U., Lorch T., Braselmann H., Engert D., Figel M., and Bauchinger M. Technical report: application of the Metafer2 fluorescence scanning system for the analysis of radiation-induced chromosome aberrations measured by FISH-chromosome painting. Mutat. Res. 492 (2001) 51-57
4. Barker P.E., Wang W., Wagner P.D., and Pinsky P. Inter-rater agreement on chromosome 5 breakage in FISH-based mutagen sensitivity assays (MSAs). Mutat. Res. 562 (2004) 133-142
5. Liehr T., Starke H., Weise A., Lehrer H., and Claussen U. Multicolor FISH probe sets and their applications. Histol. Histopathol. 19 (2004) 229-237
6. Horvathova E., Dusinska M., Shaposhnikov S., and Collins A.R. DNA damage and repair measured in different genomic regions using the comet assay with fluorescent in situ hybridization. Mutagenesis 19 (2004) 269-276
7. Santos S.J., Singh N.P., and Natarajan A.T. Fluorescence in situ hybridization with comets. Exp. Cell Res. 232 (1997) 407-411
8. Collins A.R. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol. Biotechnol. 26 (2004) 249-261
9. Olive P.L., and Banath J.P. Detection of DNA double-strand breaks through the cell cycle after exposure to X-rays, bleomycin, etoposide and 125IdUrd. Int. J. Radiat. Biol. 64 (1993) 349-358
10. Merk O., and Speit G. Detection of crosslinks with the comet assay in relationship to genotoxicity and cytotoxicity. Environ. Mol. Mutagen. 33 (1999) 167-172
11. Collins A.R., Harrington V., Drew J., and Melvin R. Nutritional modulation of DNA repair in a human intervention study. Carcinogenesis 24 (2003) 511-515
12. Martin F.L., Cole K.J., Orme M.H., Grover P.L., Phillips D.H., and Venitt S. The DNA repair inhibitors hydroxyurea and cytosine arabinoside enhance the sensitivity of the alkaline single-cell gel/electrophoresis 'comet' assay in metabolically-competent MCL-5 cells. Mutat. Res. 445 (1999) 21-43
13. Glei M., Schaeferhenrich A., Claussen U., Kuechler A., Liehr T., Weise A., Marian B., Sendt W., and Pool-Zobel B.L. Comet fluorescence in situ hybridization analysis for oxidative stress-induced DNA damage in colon cancer relevant genes. Toxicol. Sci. 96 (2007) 279-284
14. Arutyunyan R., Rapp A., Greulich K.O., Hovhannisyan G., Haroutiunian S., and Gebhart E. Fragility of telomeres after bleomycin and cisplatin combined treatment measured in human leukocytes with the Comet-FISH technique. Exp. Oncol. 27 (2005) 38-42
15. Kas E., and Chasin L.A. Anchorage of the Chinese hamster dihydrofolate reductase gene to the nuclear scaffold occurs in an intragenic region. J. Mol. Biol. 198 (1987) 677-692
16. Anderson D., Yu T.W., and Browne M.A. The use of the same image analysis system to detect genetic damage in human lymphocytes treated with doxorubicin in the Comet and fluoresence in situ hybridisation (FISH) assays. Mutat. Res. 390 (1997) 69-77
17. Giannotti E., Vandin L., Repeto P., and Comelli R. A comparison of the in vitro Comet assay with the in vitro chromosome aberration assay using whole human blood or Chinese hamster lung cells: validation study using a range of novel pharmaceuticals. Mutagenesis 17 (2002) 163-170
18. Monteith D.K., and Vanstone J. Comparison of the microgel electrophoresis assay and other assays for genotoxicity in the detection of DNA damage. Mutat. Res. 345 (1995) 97-103
19. Wasserman K. Intragenomic heterogeneity of DNA damage formation and repair: a review of cellular responses to covalent drug DNA formation. Crit. Rev. Toxicol. 24 (1996) 281-322
20. Pfeiffer P., Goedecke W., and Obe. G. Mechanisms of DNA double-strand break repair and their potential to induce chromosomal aberrations. Mutagenesis 15 (2000) 289-302
21. Rapp A., Bock C., Dittmar H., and Greulich K.O. UV-A breakage sensitivity of human chromosomes as measured by COMET-FISH depends on gene density and not on the chromosome size. J. Photochem. Photobiol. B 56 (2000) 109-117
22. Ooi.F A. Oncogene amplification detection by fluorescence in situ hybridization (FISH). Acta Histochem. Cytochem. 34 (2001) 391-397
23. Schäferhenrich A., Beyer-Sehlmeyer G., Festag G., Kuechler A., Haag N., Weise A., Liehr T., Claussen U., Marian B., Sendt W., Scheele J., and Pool-Zobel B.L. Human adenoma cells are highly susceptible to the genotoxic action of 4-hydroxy-2-nonenal. Mutat. Res. 9496 (2003) 1-14
24. McKelvey-Martin V.J., Ho E.T., McKeown S.R., Johnston S.R., McCarthy P.J., Rajab N.F., and Downes C.S. Emerging applications of the single cell gel electrophoresis (Comet) assay. I. Management of invasive transitional cell human bladder carcinoma. II Fluorescent in situ hybridization Comets for the identification of damaged and repaired DNA sequences in individual cells. Mutagenesis 13 (1998) 1-8
25. Bock C., Rapp A., Dittmar H., Monajembashi S., and Greulich K.O. Localisation of specific sequences and DNA single strand breaks in individual UV-A irradiated human lymphocytes by COMET FISH. Prog. Biomed. Optics 3568 (1999) 207-217
26. McKenna D.J., Rajab N.F., McKeown S.R., McKerr G., and Kelvey-Martin V.J. Use of the comet-FISH assay to demonstrate repair of the TP53 gene region in two human bladder carcinoma cell lines. Radiat. Res. 159 (2003) 49-56
27. Mellon I., Bohr V.A., Smith C.A., and Hanawalt P.C. Preferential DNA repair of an active gene in human cells. Proc. Natl. Acad. Sci. U.S.A. 83 (1986) 8878-8882
28. Schaeferhenrich A., Beyer-Sehlmeyer G., Festag G., Kuechler A., Haag N., Weise A., Liehr T., Claussen U., Marian B., Sendt W., Scheele J., and Pool-Zobel B.L. Human adenoma cells are highly susceptible to the genotoxic action of 4-hydroxy-2-nonenal. Mutat. Res. 526 (2003) 19-32
29. Harreus U.A., Kleinsasser N.H., Zieger S., Wallner B., Reiter M., Schuller P., and Berghaus A. Sensitivity to DNA-damage induction and chromosomal alterations in mucosa cells from patients with and without cancer of the oropharynx detected by a combination of Comet assay and fluorescence in situ hybridization. Mutat. Res. 563 (2004) 131-138
30. Tirukalikundram S., Kumaravel T.S., and Bristow R.G. Detection of genetic instability at HER-2/neu and p53 loci in breast cancer cells using Comet-FISH. Breast Cancer Res. Treat. 91 (2005) 89-93
31. Fernandez J.L., Vazquez-Gundin F., Rivero M.T., Genesca A., Gosalvez J., and Goyanes V. DBD-fish on neutral comets: simultaneous analysis of DNA single- and double-strand breaks in individual cells. Exp. Cell Res. 270 (2001) 102-109
32. Slijepcevic P., and Natarajan A.T. Distribution of X-ray-induced G2 chromatid damage among Chinese hamster chromosomes: influence of chromatin conformation. Mutat. Res. 323 (1994) 113-119
33. Slijepcevic P., and Natarajan A.T. Distribution of radiation-induced G1 exchange and terminal deletion breakpoints in Chinese hamster chromosomes as detected by G banding. Int. J. Radiat. Biol. 66 (1994) 747-755
34. Tucker J.D., and Senft J.R. Analysis of naturally occurring and radiation-induced breakpoint locations in human chromosomes 1, 2 and 4. Radiat. Res. 140 (1994) 31-36
35. Rapp A., Hausmann M., and Greulich K.O. The comet-FISH technique: a tool for detection of specific DNA damage and repair. Methods Mol. Biol. 291 (2005) 107-119
36. Schäferhenrich A., Sendt W., Scheele J., Kuechler A., Liehr T., Claussen U., Rapp A., Greulich K.-O., and Pool-Zobel B.L. Putative colon cancer risk factors damage global DNA and TP53 in primary human colon cells isolated from surgical samples. Food Chem. Toxicol. 41 (2003) 655-664
37. Knöbel Y., Weise A., Glei M., Sendt W., Claussen U., and Pool-Zobel B.L. Ferric iron is genotoxic in non-transformed and preneoplastic human colon cells. Food Chem. Toxicol. 45 (2007) 804-811
38. Collins A.R., Dobson V.L., Dusinska M., Kennedy G., and Stetina R. The comet assay: what can it really tell us?. Mutat. Res. 375 (1997) 183-193
39. Wollowski I., Ji S., Bakalinsky A.T., Neudecker C., and Pool-Zobel B.L. Bacteria used for the production of yogurt inactivate carcinogens and prevent DNA damage in the colon of rats. J. Nutr. 129 (1999) 77-82
40. Schaeferhenrich A., Sendt W., Scheele J., Kuechler A., Liehr T., Claussen U., Rapp A., Greulich K.O., and Pool-Zobel B.L. Putative colon cancer risk factors damage global DNA and TP53 in primary human colon cells isolated from surgical samples. Food Chem. Toxicol. 41 (2003) 655-664
41. Knobel Y., Glei M., Weise A., Osswald K., Schaferhenrich A., Richter K.K., Claussen U., and Pool-Zobel B.L. Uranyl nitrilotriacetate, a stabilized salt of uranium, is genotoxic in nontransformed human colon cells and in the human colon adenoma cell line LT97. Toxicol. Sci. 93 (2006) 286-297
42. Park E., Glei M., Knobel Y., and Pool-Zobel B.L. Blood mononucleocytes are sensitive to the DNA damaging effects of iron overload-in vitro and ex vivo results with human and rat cells. Mutat. Res. 619 (2007) 59-67
43. Fearon E.R. Human cancer syndromes: clues to the origin and nature of cancer. Science 278 (1997) 1043-1050
44. Fearon E.R., and Vogelstein B. A genetic model for colorectal tumorigenesis. Cell 61 (1990) 759-767
45. Eisenbrand G., and Tang W. Food-borne heterocyclic amines. Chemistry, formation, occurrence and biological activities. A literature review. Toxicology 84 (1993) 1-82
46. Erhardt J.G., Lim S.S., Bode J.C., and Bode C. A diet rich in fat and poor in dietary fiber increases the in vitro formation of reactive oxygen species in human feces. J. Nutr. 127 (1998) 706-709
47. Lund E.K., Wharf S.G., Fairweather-Tait S.J., and Johnson I.T. Oral ferrous sulfate supplements increase the free radical-generating capacity of feces from healthy volunteers. Am. J. Clin. Nutr. 69 (1999) 250-255
48. Abrahamse S.L., Pool-Zobel B.L., and Rechkemmer G. Potential of short chain fatty acids to modulate the induction of DNA damage and changes in the intracellular calcium concentration in isolated rat colon cells. Carcinogenesis 20 (1999) 629-634
49. Glei M., Klenow St., Sauer J., Wegewitz U., Richter K., and Pool-Zobel B.L. Hemoglobin and hemin induce DNA damage in human colon tumor cell line HT29 clone 19A and in primary human colonocytes. Mutat. Res. (2005)
50. Glei M., Latunde-Dada G.O., Klinder A., Becker T.W., Hermann U., Voigt K., and Pool-Zobel B.L. Iron-overload induces oxidative DNA damage in the human colon carcinoma cell line HT29 clone 19A. Mutat. Res. 519 (2002) 151-161
51. 2-(1,3-propano)-2′-deoxygunanosine adducts in mammalian cells. Chem. Res. Toxicol. 9 (1996) 1207-1213
52. Huang X. Iron overload and its association with cancer risk in humans: evidence for iron as a carcinogenic metal. Mutat. Res. 533 (2003) 153-171
53. Babbs C.F. Oxygen radicals in ulcerative colitis. Free Radical Biol. Med. 13 (1992) 169-181
54. Imlay J.A., and Linn S. DNA damage and oxygen radical toxicity. Science 240 (1988) 1302-1309
55. Taqui Khan M.M., and Martell A.E. Kinetics of metal ion and metal chelate catalyzed oxidation of ascorbic acid. IV. Uranyl ion catalyzed oxidation. J. Am. Chem. Soc. 91 (1969) 4668-4672
56. Hamilton M.M., Ejnik J.W., and Carmichael A.J. Uranium reactions with hydrogen peroxide studies by EPR-spin trapping with DMPO. J. Chem. Soc. Perkin Trans. 2 (1997) 2491-2494
57. Cerutti P.A. Prooxidant states and tumor promotion. Science 227 (1985) 375-381
58. Dreher D., and Junod A.F. Role of oxygen free radicals in cancer development. Eur. J. Cancer 32A (1996) 30-38
59. Dittberner U., Schmetzer B., Gölzer P., and Eisenbrand G. Genotoxic effects of 2-trans-hexenal in human buccal mucosa cells in vivo. Mut. Res. Genet. Toxicol. 390 (1997) 161-165
60. Esterbauer H., Eckl P., and Ortner A. Possible mutagens derived from lipids and lipid precursors. Mutat. Res. 238 (1990) 223-233
61. Chung F.L., Chen H.J.C., Guttenplan J.B., Nishikawa A., and Hard G.C. 2,3-Epoxy-4-hydroxynonanal as a potential tumor-initiating agent of lipid peroxidation. Carcinogenesis 14 (1993) 2073-2077
62. Chung F.L., Candy H.J., and Nath R.G. Lipid peroxidation as a potential endogenous source for the formation of exocyclic DNA adducts. Carcinogenesis 17 (1996) 2105-2111
63. 2-ethenoguanine and 5,6,7,9-tetrahydro-7-hydroxy-9-oxoimidazo[1,2-α]purine in oligonucleotides by Escherichia coli polymerases I exo- and II exo- T7 polymerase exo-, human immunodeficiency virus-1-reverse transcriptase, and rat polymerase ß. Biochemistry 36 (1997) 6069-6079
64. 2-ethenoguanine in Chinese hamster ovary cell chromosomal DNA. Chem. Res. Toxicol. 12 (1999) 501-507
65. Liehr T., Heller A., Starke H., Rubtsov N., Trifonov V., Mrasek K., Weise A., Kuechler A., and Claussen U. Microdissection based high resolution multicolour banding (MCB) for all 24 human chromosomes. Int. J. Mol. Med. 9 (2002) 335-339
66. Baker S.J., Fearon E.R., Nigro J.M., Hamilton S.R., Preisinger A.C., Jessup J.M., vanTuinen P., Ledbetter D.H., Barker D.F., and Nakamura Y. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science 244 (1989) 217-221
67. Barbin A. Etheno-adduct forming chemicals: from mutagenicity testing to tumor mutation spectra. Mutat. Res. 462 (2002) 55-69
68. Liehr T., Heller A., Starke H., Rubstov N., Trifonov V., Mrasek K., Weise A., Kuechler A., and Claussen U. Microdissection based high resolution multicolour banding (MCB) for all 24 chromosomes. Int. J. Mol. Med. 9 (2002) 335-339
69. Y. Knöbel, A. Weise, M. Glei, W. Sendt, U. Claussen, B.L. Pool-Zobel. Ferric iron is genotoxic in non transformed and preneoplastic human colon cells, Food Chem. Toxicol. 45 (2007) 804-811.
70. Arutyunyan R., Gebhart E., Hovhannisyan G., Greulich K.O., and Rapp A. Comet-FISH using peptide nucleic acid probes detects telomeric repeats in DNA damaged by bleomycin and mitomycin C proportional to general DNA damage. Mutagenesis 19 (2004) 403-408
71. Hovhannisyan G., Rapp A., Arutyunyan R., Greulich K.O., and Gebhart E. Comet-assay in combination with PNA-FISH detects mutagen-induced DNA damage and specific repeat sequences in the damaged DNA of transformed cells. Int. J. Mol. Med. 15 (2005) 437-442
72. Ishibashi T., and Lippard S.J. Telomere loss in cells treated with cisplatin. Proc. Natl. Acad. Sci. U.S.A. 95 (1998) 4219-4223
73. Carrasco C., Rosu F., Gabelica V., Houssier C., De P.E., Garbay-Jaureguiberry C., Roques B., Wilson W.D., Chaires J.B., Waring M.J., and Bailly C. Tight binding of the antitumor drug ditercalinium to quadruplex DNA. Chembiochem 3 (2002) 1235-1241
74. Schmezer P., Rajaee-Behbahani N., Risch A., Thiel St., Rittgen W., Drings P., Dienemann H., Kayser K.W., Schulz V., and Bartsch H. Rapid screening assay for mutagen sensitivity and DNA repair capacity in human peripheral blood lymphocytes. Mutagenesis 16 (2001) 25-30
75. Frankenberg-Schwager M. Review of repair kinetics for DNA damage induced in eukaryotic cells in vitro by ionizing radiation. Radiother. Oncol. 14 (1989) 307-320
76. Collins A.R., and Horvathova E. Oxidative DNA damage, antioxidants and DNA repair: applications of the comet assay. Biochem. Soc. Trans. 29 (2001) 337-341
77. Fuss J.O., and Cooper P.K. DNA repair: dynamic defenders against cancer and aging. PLoS. Biol. 4 (2006) e203
78. Venema J., Bartosova Z., Natarajan A.T., van Zeeland A.A., and Mullenders L.H. Transcription affects the rate but not the extent of repair of cyclobutane pyrimidine dimers in the human adenosine deaminase gene. J. Biol. Chem. 267 (1992) 8852-8856
79. Taffe B.G., Larminat F., Laval J., Croteau D.L., Anson R.M., and Bohr V.A. Gene-specific nuclear and mitochondrial repair of formamidopyrimidine DNA glycosylase-sensitive sites in Chinese hamster ovary cells. Mutat. Res. 364 (1996) 183-192
80. McKenna D.J., Gallus M., McKeown S.R., Downes C.S., and McKelvey-Martin V.J. Modification of the alkaline Comet assay to allow simultaneous evaluation of mitomycin C-induced DNA cross-link damage and repair of specific sequences in RT4 cells. DNA Repair 2 (2003) 879-890
81. Ruven H.J., Seelen C.M., Lohman P.H., van K.H., van Zeeland A.A., and Mullenders L.H. Strand-specific removal of cyclobutane pyrimidine dimers from the p53 gene in the epidermis of UVB-irradiated hairless mice. Oncogene 9 (1994) 3427-3432
82. Evans M.K., Taffe B.G., Harris C.C., and Bohr V.A. DNA strand bias in the repair of the p53 gene in normal human and xeroderma pigmentosum group C fibroblasts. Cancer Res. 53 (1993) 5377-5381
83. Glei M., Habermann N., Osswald K., Seidel C., Persin C., Jahreis G., and Pool-Zobel B.L. Assessment of DNA damage and its modulation by dietary and genetic factors in smokers using the Comet assay: a biomarker model. Biomarkers 10 (2005) 203-217
84. Keen J.C., and Davidson N.E. The biology of breast carcinoma. Cancer 97 (2003) 825-833
85. Berwick M., and Vineis P. Markers of DNA repair and susceptibility to cancer in humans: an epidemiologic review. J. Natl. Cancer Inst. 92 (2000) 874-897
86. Winston J.S., Ramanaryanan J., and Levine E. HER-2/neu evaluation in breast cancer are we there yet?. Am. J. Clin. Pathol. 121 Suppl. (2004) S33-S49
87. Gasco M., Yulug I.G., and Crook T. TP53 mutations in familial breast cancer: functional aspects. Hum. Mutat. 21 (2003) 301-306
88. Amendola R., Basso E., Pacifici P.G., Piras E., Giovanetti A., Volpato C., and Romeo G. Ret, Abl1 (cAbl) and Trp53 gene fragmentations in comet-FISH assay act as in vivo biomarkers of radiation exposure in C57BL/6 and CBA/J mice. Radiat. Res. 165 (2006) 553-561