A potent anti-oxidant property: Fluorescent recombinant α-phycocyanin of Spirulina

Journal of Applied Microbiology

Volumn 106, Issue 4, 2009, Pages 1093-1100

  Guan, X Y ^a   Zhang, W J ^b,c   Zhang, X W ^b,c   Li, Y X ^b,c   Wang, J F ^b,c   Lin, H Z ^b,c   Tang, X X ^a   Qin, S ^b  

^a OCEAN UNIVERSITY OF CHINA   (China)

^b INSTITUTE OF OCEANOLOGY   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Antioxidant; Phycocyanin; RHHPC; Spirulina platensis

Indexed keywords

ABSORBANCE; BIOCHEMISTRY; CHROMOPHORES; EMISSION SPECTROSCOPY; ESCHERICHIA COLI; FLUORESCENCE;

ANTIOXIDANT PROPERTIES; BENCH SCALE; FEEDING MODES; HIS-TAG; PEROXYL RADICAL; PHYCOCYANIN; RHHPC; SPIRULINA; SPIRULINA PLATENSIS; SYNECHOCYSTIS SP;

ANTIOXIDANTS;

CYSTEINE; HYDROXYL RADICAL; LYASE; NICKEL; PEROXY RADICAL; PHYCOCYANIN; PLASMID VECTOR; POLYHISTIDINE TAG; RECOMBINANT ALPHA PHYCOCYANIN; SCAVENGER; UNCLASSIFIED DRUG;

ANTIOXIDANT; BIOACTIVITY; BIOMASS; BIOTRANSFORMATION; CATALYSIS; COLIFORM BACTERIUM; CYANOBACTERIUM; CYST; FLUORESCENCE; PIGMENT; PROTEIN; RADICAL; RECOMBINATION;

ABSORPTION SPECTROPHOTOMETRY; ANTIOXIDANT ACTIVITY; ARTICLE; BIOMASS; CHROMATOPHORE; CONTROLLED STUDY; DRUG PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUORESCENCE; GENE CASSETTE; GENE EXPRESSION; IC 50; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTIDE SYNTHESIS; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SPIRULINA PLATENSIS; SYNECHOCYSTIS;

EID: 62149090962     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2008.04051.x     Document Type: Article

References (41)

1. Apt, K.E., Collier, J.L. Grossman, A.R. (1995) Evolution of the phycobiliproteins. J Mol Biol 248, 79 96.
2. Berkelman, T. Lagarias, J.C. (1986) Visualization of bilin-linked peptides and proteins in polyacrylamide gels. Anal Biochem 156, 194 201.
3. Bermejo, R., Talavera, E.M., Alarez-Pez, J.M. Orte, J.C. (1997) Chromatographic purification of biliproteins from Spirulina plarensis, high performance liquid chromatographic separation of their and subunits. J Chromatogr A 778, 441 450.
4. Bhat, V.B. Madyastha, K.M. (2000) C-Phycocyanin: a potent peroxyl radical scavenger in vivo and in vitro. Biochem Biophys Res Commun 275, 20 25.
5. Finkel, T. Holbrook, N.J. (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408, 239 247.
6. Ge, B.S., Tang, Z.H., Zhao, F.Q., Ren, Y.H., Yang, Y. Qin, S. (2005) Scale-up of the fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli. Process Biochem 40, 3190 3195.
7. Ge, B.S., Qin, S., Han, L., Lin, F. Ren, Y.H. (2006) Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli. J Photochem Photobiol B 84, 175 180.
8. Georgiou, G., Bahra, S.S., Mackie, A.R., Wolfe, C.A., O'Shea, P., Ladha, S., Fernandez, N. Cherry, R.J. (2002) Measurement of the lateral diffusion of human MHC class I molecules on HeLa cells by fluorescence recovery after photobleaching using a phycoerythrin probe. Biophys J 82, 1828 1834.
9. Grossman, A.R., Schaefer, M.R., Chiang, G.G. Collier, J.L. (1993) The Phycobilisome, a light-harvesting complex responsive to environmental conditions. Microbiol Rev 57, 725 749.
10. Guan, X.Y., Qin, S., Su, Z.L., Zhao, F.Q., Ge, B.S., Li, F.C. Tang, X.X. (2007) Combinational biosynthesis of a fluorescent cyanobacterial holo-α-phycocyanin in Escherichia coli by using one expression vector. Appl Biochem Biotech 142, 52 59.
11. Gupta, R., Sharma, P. Vyas, V.V. (1995) Effect of growth environment on the stability of a recombinant shuttle plasmid, pCPPS-31, in Escherichia coli. J Biotechnol 41, 29 37.
12. Gutteridge, J.M. Halliwell, B. (1988) The deoxyribose assay: an assay both for 'free' hydroxyl radical and for site-specific hydroxyl radical production. Biochem J 253, 932 933.
13. Huang, Z., Guo, B.J., Wong, R.N.S. Jiang, Y. (2007) Characterization and antioxidant activity of selenium-containing phycocyanin isolated from Spirulina platensis. Food Chem 100, 1137 1143.
14. Jorgensen, L.V., Andersen, H.J. Skibsted, L.H. (1997) Kinetics of reduction of hypervalent iron in myoglobin by crocin in aqueous solution. Free Radic Res 27, 73 87.
15. Kehrer, J.P. (1993) Free radicals as mediators of tissue injury and disease. Crit Rev Toxicol 23, 21 48.
16. Klein, J.A. Ackerman, S.L. (2003) Oxidative stress, cell cycle, and neurodeneneration. J Clin Invest 111, 785 793.
17. Lissi, E.A., Pizarro, M., Aspee, A. Romay, C. (2000) Kinetics of phycocyanine bilin groups destruction by peroxyl radicals. Free Rad Bio Med 28, 1051 1055.
18. MacColl, R. (1998) Cyanobacterial phycobilisomes. J Struct Biol 124, 311 334.
19. McCord, J. (2003) Oxidative stress-related disease overview. In Critical Reviews of Oxidative Stress and Aging ed. Cutler, R. Rodriguez, H. pp. 487 502. Singapore : World Scientific Publishing.
20. Minkova, K.M., Tchernov, A.A., Tchorbadjieva, M.I., Fournadjieva, S.T., Antova, R.E. Busheva, M.C. (2003) Purification of C-phycocyanin from Spirulina (Arthrospira) fusiformis source. J Biotechnol 102, 55 59.
21. Morcos, N.C. (1988) Phycocyanin laser activation cytotoxic effect and uptake in human atherosclerotic plaque. Lasers Surg Med 8, 7 10.
22. Niu, Z.Y., Pan, L., Liu, Y.J., Zhang, X.J. Suo, X.H. (2006) Effects of integrin beta1 on phycocyanin inhibiting proliferation of K562 cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi (J Exper Hemat) 14, 658 661.
23. Pinero Estrada, J.E., Bermejo Bescos, P. Villar del Fresno, A.M. (2001) Antioxidant activity of different fractions of Spirulina platensis protean extract. Farmaco 56, 497 500.
24. Priet, B., Bonizzi, C. Merville, M.P. (1998) NFKB transcription factor activation: inportant of the redox regulation. In Oxidative Stress in Cancer, AIDS and Neurodegenerative Diseases ed. Montagnier, L. et al. pp. 63 74. NY : Marcell Dekker Presss.
25. Riss, J., Décordé, K., Sutra, T., Delage, M., Baccou, J.C., Jouy, N., Brune, J.P., Oréal, H. et al. (2007) Phycobiliprotein C-phycocyanin from Spirulina platensis is powerfully responsible for reducing oxidative stress and NADPH oxidase expression induced by an atherogenic diet in hamsters. J Agric Food Chem 55, 7962 7967.
26. Robert, M. Leslie, E.A.M. (2003) Allophycocyanin: trimers, monomers, subunits, and homodimers. Biopolymers 72, 352 365.
27. Romay, C., Armesto, J., Remirez, D., Gonzalez, R., Ledon, N. Garcia, I. (1998) Antioxidant and anti-inflammatory properties of C-phycocyanin from blue-green algae. Inflamm Res 47, 36 41.
28. Romay, Ch., Gonzalez, R., Ledon, N., Remirez, D. Rimbau, V. (2003) C-phycocyanin: a biliprotein with antioxidant, antiinflammatory and neuroprotective effects. Curr Protein Pept Sci 4, 207 216.
29. Sambrook, J. Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press.
30. Serena, B., Francesca, B., Silvia, P., Sonia, F., Stefano, S. Franco, C. (2004) Antioxidant properties of a novel phycocyanin extract from the bluegreen alga Aphanizomenon flos-aquae. Life Sci 75, 2353 2362.
31. Shen, G., Saunée, N.A., Williams, S.R., Gallo, E.F., Schluchter, W.M. Bryant, D.A. (2006) Identification and characterization of a new class of bilin lyase responsible for attachment of phycocyanobilin to Cys-153 on the b-subunit of phycocyanin in Synechococcus sp. PCC 7002. J Biol Chem 27, 17768 17778.
32. Sidler, W.A. (1994) Phycobilisome and phycobiliprotein structures. In The Molecular Biology of Cyanobacteria ed. Bryant, D.A. pp. 141 151. Dordrecht, Netherlands : Kluwer Academic Publishers.
33. Subhashini, J., Mahipal, S.V., Reddy, M.C., Mallikarjuna Reddy, M., Rachamallu, A. Reddanna, P. (2004) Molecular mechanisms in C-Phycocyanin induced apoptosis in human chronic myeloid leukemia cell line-K562. Biochem Pharmacol 68, 453 462.
34. Sun, L., Wang, S.M., Chen, L.X. Gong, X.Q. (2003) Promising fluorescent probes from phycobiliproteins. IEEE J Quantum Electron 9, 177 188.
35. Sun, L., Wang, S.M. Qiao, Z.Y. (2006) Chemical stabilization of the phycocyanin from cyanobacterium Spirulina platensis. J Biotechnol 121, 563 569.
36. Swanson, R.V., Zhou, J., Leary, J.A., Williams, T., de Lorimier, R., Bryant, D.A. Glazer, A.N. (1992) Characterization of phycocyanin produced by cpcE and cpcF mutants and identification of an intergenic suppressor of the defect in bilin attachment. J Biol Chem 267, 16146 16154.
37. Tooley, A.J., Cai, Y.A. Glazer, A.N. (2001) Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-α subunit in a heterologous host. Proc Natl Acad Sci USA 98, 60 65.
38. Wang, H., Liu, Y., Gao, X., Carter, C.L. Liu, Z.R. (2007) The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis. Cancer Lett 247, 150 158.
39. Waris, G. Ahsan, H. (2006) Reactive oxygen species: role in the development of cancer and various chronic conditions. Carcinogenesis 5, 14.
40. Winyard, P.G., Moody, C.J. Jacob, C. (2005) Oxidative activation of antioxidant defence. Trends Biochem Sci 30, 453 461.
41. Zhao, K.H., Su, P., Li, J., Tu, J.M., Zhou, M., Bubenzer, C. Scheer, H. (2006) Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin: cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120. J Biol Chem 281, 8573 8581.